Objective      To synthesize nanoparticles (NP) with follicle-stimulating hormone receptor peptide (FSHR

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) encapsulated by poly(lactic acid-glycolic acid) (PLGA)

which is biodegradable polymer material approved by Food and Drug Administration

and explore whether PLGA carrier is suitable for contraceptive vaccine.       Methods      Emulsification process was used for preparation of FSHR

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-encapsulated PLGA NP (FSHR

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/PLGA NP)

and the mo

rphology was observed with a transmission electron microscope. The particle size distribution and Zeta potential were detected with a laser potential particle size instrument

and the in vitro releasing characteristic of the NP was studied. A laser scanning confocal microscope was employed to observe the uptake capacity of bone marrow-derived dendritic cells (BMDCs) to soluble FSHR

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and FSHR

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/PLGA NP

and a flow cytometer was used to detect the phenotypic changes of BMDCs co-cultured with FSHR

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PLGA NP and FSHR

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/PLGA NP

respectively.       Results      FSHR

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/PLGA NP had uniform particle size distribution and regular surface

and could slowly release antigens

with average particle size of 296.3±3.5 nm

Zeta potential of -16.1±0.4 mV

FSHR

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encapsulation efficiency of  62.71%±2.83%

and total release rate of 98.3%. The in vitro cell test showed that FSHR

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was easily up-taken by BMDCs in a dose- and time-dependent manner

and the expressions of CD40

CD86

CD83 and MHC-Ⅱ on the surface of BMDCs were up-regulated.       Conclusion      The FSHR

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/PLGA NP have good encapsulation efficiency

and can be easily up-taken by BMDCs to promote maturation of BMDCs. PLGA can be used as the carrier of FSHR

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contraceptive vaccine.