Objective      To investigate the expression and relationship of human papillomavirus (HPV) E6

wild type p53

and PinX1 in cervical squamous carcinoma tissues

adjacent tissues

and normal cervical tissues.       Methods      Seventeen cases of cervical squamous carcinoma and adjacent tissues were collected from Department of Obstetrics and Gynecology

Daping Hospital of the Third Military Medical University during March 2013 to February 2014

and 10 cases of normal cervical tissues were selected as control. Real-time PCR

Western blotting and immunohistochemistry assay were used to detect the expression and distribution of HPV E6

wild type p53

and PinX1 in cervical squamous carcinoma tissues

adjacent tissues

and normal cervical tissues.       Results      The mRNA and protein levels of HPV E6 in the cervical squamous carcinoma tissues were significantly higher than those in the adjacent mucosa (P0.05)

and the expression levels of wild type p53 and PinX1 were dramatically decreased compared with adjacent and normal mucosa (P0.05).   HPV E6 had a negative correlation with the decreased expression levels of wild type p53 (R=-0.605

P0.01) and PinX1 (R=-0.496

P0.05)  in cervical squamous carcinoma

however

a positive correlation was seen between the expression levels of wide type p53 and PinX1 (R=0.824

P0.01).       Conclusion      HPV E6 expression is negatively related with wild type p53 and PinX1 in cervical squamous carcinoma

and PinX1 is closely related with the expression of wild type p53. HPV E6 may result in the carcinogenesis and development of cervical squamous carcinoma through inhibiting wild type p53 and PinX1.