Effect of over-expression of SPAG9 on proliferation, cell cycle and migration in human prostate cancer cell line PC3

龙洲; 刘骞; 朱景振; 赵江; 董兴有; 冯观贵; 丁国林; 李龙坤

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Effect of over-expression of SPAG9 on proliferation, cell cycle and migration in human prostate cancer cell line PC3

更新时间：2026-01-09

- Effect of over-expression of SPAG9 on proliferation, cell cycle and migration in human prostate cancer cell line PC3
- Journal of Army Medical University Vol. 37, Issue 10, Pages: 1012-1016(2015)
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  CLC： R341;R730.23;R737.25
- Published：2015
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龙洲,刘骞,朱景振,赵江,董兴有,冯观贵,丁国林,李龙坤.过表达精子相关抗原9对前列腺癌细胞PC3生物学行为的影响[J].陆军军医大学学报(原第三军医大学学报),2015,37(10):1012-1016.

　Long Zhou,Liu Qian,Zhu Jingzhen,Zhao Jiang,Dong Xingyou,Feng Guangui,Ding Guolin,Li Longkun.Effect of over-expression of SPAG9 on proliferation, cell cycle and migration in human prostate cancer cell line PC3[J].J Amry Med Univ (J Third Mil Med Univ),2015,37(10):1012-1016.
龙洲,刘骞,朱景振,赵江,董兴有,冯观贵,丁国林,李龙坤.过表达精子相关抗原9对前列腺癌细胞PC3生物学行为的影响[J].陆军军医大学学报(原第三军医大学学报),2015,37(10):1012-1016. DOI：

　Long Zhou,Liu Qian,Zhu Jingzhen,Zhao Jiang,Dong Xingyou,Feng Guangui,Ding Guolin,Li Longkun.Effect of over-expression of SPAG9 on proliferation, cell cycle and migration in human prostate cancer cell line PC3[J].J Amry Med Univ (J Third Mil Med Univ),2015,37(10):1012-1016. DOI：

摘要

目的研究精子相关抗原9（sperm-associated antigen 9

SPAG9）对前列腺癌细胞增殖、细胞周期、侵袭转移等生物学行为的影响，探讨可能的分子机制。方法前列腺细胞PC3中转染SPAG9后，qRT-PCR和Western blot检测转染后细胞SPAG9的表达变化；CCK-8检测SPAG9对PC3细胞增殖的影响；流式细胞术检测SPAG9对PC3细胞周期的作用；Transwell实验检测转染SPAG9对PC3细胞体外迁移能力的影响。结果 qRT-PCR检测结果表明SPAG9组的SPAG9的相对表达量（20.776 3±3.080 9）明显高于对照组（1.010 2±0.178 4）（P0.01）。Western blot结果显示在前列腺癌细胞PC3中过表达SPAG9后成功。CCK-8实验表明SPAG9促进细胞增殖；流式细胞术检测结果表明过表达SPAG9使PC3细胞周期加快；在Transwell迁移实验中，SPAG9转染细胞后，SPAG9组穿膜细胞数为（88.078±4.509）/视野，对照组穿膜细胞数为（54.333±4.582）/视野，SPAG9组细胞迁移能力明显高于对照组（P0.05）。结论 SPAG9促进前列腺癌细胞PC3的增殖、细胞周期及侵袭转移等生物学行为，并可能通过ERK和JNK信号通路发挥这些作用。

Abstract

Objective To investigate the effect of sperm-associated antigen 9 (SPAG9) over-expression on the proliferation

cell cycle and migration in human prostate cancer cell line PC3 and the relevant molecular mechanism. Methods PC3 prostate cancer cells were transformed with plasmid M29-SPAG9 and plasmid M29

and qRT-PCR and Western blotting were used to detect the SPAG9 expression levels. The cell proliferation was detected by CCK-8 assay. The ce

ll cycle distribution was analyzed by flow cytometry. Transwell migration assay was used to analyze cell migration ability. Results The results of qRT-PCR and Western blot analysis indicated that the SPAG9 expression in the M29-SPAG9 over-expression group was higher than that of the M29 control group (20.776 3±3.080 9 vs 1.010 2±0.178 4

P0.01). Compared with the M29 control group

the cell proliferation activity was significantly higher in the SPAG9 over-expression group (P0.05)

and the G

/S phase proportion was increased (P0.05). Transwell migration assay indicated that the cell migration ability of the M29-SPAG9 over-expression group was significantly higher than that of the M29 control group (88.078±4.509 vs 54.333±4.582

P0.05). Conclusion Over-expression of SPAG9 promotes the proliferation

cell cycle and migration in human prostate cancer cell line PC3 through ERK and JNK signaling pathway.

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