Rattan tea extracts improve insulin resistance in type 2 diabetes rats

吴瑛; 吴彬; 万婧; 张勇; 朱俊东; 周永; 糜漫天

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Rattan tea extracts improve insulin resistance in type 2 diabetes rats

更新时间：2026-01-09

- Rattan tea extracts improve insulin resistance in type 2 diabetes rats
- Journal of Army Medical University Vol. 37, Issue 5, Pages: 454-458(2015)
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  CLC： R-332;R285.5;R587.105
- Published：2015
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吴瑛,吴彬,万婧,张勇,朱俊东,周永,糜漫天.藤茶提取物改善2型糖尿病大鼠胰岛素抵抗的作用[J].陆军军医大学学报(原第三军医大学学报),2015,37(05):454-458.

　Wu Ying,Wu Bin,Wan Jing,Zhang Yong,Zhu Jundong,Zhou Yong,Mi Mantian.Rattan tea extracts improve insulin resistance in type 2 diabetes rats[J].J Amry Med Univ (J Third Mil Med Univ),2015,37(05):454-458.
吴瑛,吴彬,万婧,张勇,朱俊东,周永,糜漫天.藤茶提取物改善2型糖尿病大鼠胰岛素抵抗的作用[J].陆军军医大学学报(原第三军医大学学报),2015,37(05):454-458. DOI：

　Wu Ying,Wu Bin,Wan Jing,Zhang Yong,Zhu Jundong,Zhou Yong,Mi Mantian.Rattan tea extracts improve insulin resistance in type 2 diabetes rats[J].J Amry Med Univ (J Third Mil Med Univ),2015,37(05):454-458. DOI：

摘要

目的观察藤茶提取物对2型糖尿病（T2DM）大鼠胰岛素抵抗的改善作用及可能的分子机制。方法雄性SD大鼠高脂喂养联合小剂量链脲佐菌素腹腔注射（STZ

30 mg/kg）建立T2DM大鼠模型。建模成功后将T2DM大鼠系统抽样随机分为糖尿病模型对照组（DIA）、二甲双胍干预组（MET，125 mg/kg）、二氢杨梅素干预组（DHM，100 mg/kg）、藤茶提取物低剂量干预组（R50，50 mg/kg）、中剂量干预组（R100，100 mg/kg）及高剂量干预组（R200，200 mg/kg）6组。每组大鼠分别灌胃给药干预4周后，放射免疫分析法测定大鼠血清中胰岛素、胰岛素C肽；Western blot 法检测肝脏组织中p-Akt、FGF21、p-AMPK蛋白表达；qRT-PCR法检测肝脏组织中FGF21 mRNA的表达。结果与DIA组相比，二甲双胍、DHM和藤茶提取物干预组大鼠血糖、血清胰岛素水平、HOMA-IR指数显著降低（P0.05），而胰岛素C肽水平显著升高（P0.05）；同时肝脏组织中p-Akt、FGF21、p-AMPK蛋白及FGF21 mRNA的表达升高（P0.05）。结论藤茶提取物可通过上调p-Akt、FGF21、p-AMPK的表达从而改善2型糖尿病大鼠的胰岛素抵抗。

Abstract

Objective To determine the effects of Rattan tea on insulin resistance in rats of type 2 diabetes (T2DM) and investigate the possible underlying molecular mechanism. Methods Male SD rats (SPF) fed with high-fat diet and injected with small dose of streptozotocin (STZ) were used to establish type 2 diabetic rat model. Then

T2DM rats were randomly divided into diabetic model group (DIA)

metformin 125 mg/kg group (MET)

dihydromyricetin 100 mg/kg group (DHM)

Rattan tea extracts 50 mg/kg group (R50)

Rattan tea extracts 100 mg/kg group (R100) and Rattan tea extracts 200 mg/kg group (R200). After 4 weeks' intervention

the serum levels of insulin and insulin C-peptide

and the protein expression of p-Akt

FGF21 and p-AMPK were detected by Western blotting

as well as the mRNA expression of FGF21 in liver tissues were measured by real-time RT-PCR. Results Compared with DIA group

the levels of blood glucose

insulin and HOMA-IR were decreased significantly

meanwhile

the serum level of insulin C-peptide was increased significantly

the protein expression of p-Akt

FGF21

p-AMPK and FGF21 was up-regulated in liver tissues

as well as mRNA expression of FGF21 (P0.05). Conclusion Rattan tea improves insulin resistance in diabetic rats via up-regulating the expression of p-Akt

FGF21 and p-AMPK.

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