Effect of novel  curcumin nanoparticles on proliferation and apoptosis of Lewis lung cancer cells in vitro

李松霖; 谭群友; 王如文; 方春抒; 于杰; 祁迪

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Effect of novel curcumin nanoparticles on proliferation and apoptosis of Lewis lung cancer cells in vitro

更新时间：2026-01-09

- Effect of novel curcumin nanoparticles on proliferation and apoptosis of Lewis lung cancer cells in vitro
- Journal of Army Medical University Vol. 37, Issue 2, Pages: 141-145(2015)
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  CLC： R285.5;R730.23;R734.2
- Published：2015
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李松霖,谭群友,王如文,方春抒,于杰,祁迪.新型姜黄素纳米粒对Lewis肺癌细胞增殖、凋亡的影响[J].陆军军医大学学报(原第三军医大学学报),2015,37(02):141-145.

　Li Songlin,Tan Qunyou,Wang Ruwen,Fang Chunshu,Yu Jie,Qi Di.Effect of novel curcumin nanoparticles on proliferation and apoptosis of Lewis lung cancer cells in vitro[J].J Amry Med Univ (J Third Mil Med Univ),2015,37(02):141-145.
李松霖,谭群友,王如文,方春抒,于杰,祁迪.新型姜黄素纳米粒对Lewis肺癌细胞增殖、凋亡的影响[J].陆军军医大学学报(原第三军医大学学报),2015,37(02):141-145. DOI：

　Li Songlin,Tan Qunyou,Wang Ruwen,Fang Chunshu,Yu Jie,Qi Di.Effect of novel curcumin nanoparticles on proliferation and apoptosis of Lewis lung cancer cells in vitro[J].J Amry Med Univ (J Third Mil Med Univ),2015,37(02):141-145. DOI：

摘要

目的探讨一种新型的姜黄素纳米粒（curcumin nanoparticles，Cur-NPs）对Lewis肺癌细胞LLC增殖与凋亡的影响。方法游离姜黄素(curcumin，Cur)和Cur-NPs分别作用于LLC细胞24 h，MTT检测其对LLC细胞的增殖抑制作用。20 μmol/L的药物作用于细胞24 h后，流式细胞术检测Cur-NPs 对细胞周期、细胞凋亡、细胞内活性氧、细胞内钙离子浓度、细胞线粒体膜电位的影响，并与Cur进行对照。结果 Cur-NPs可以明显增强Cur对LLC细胞的增殖抑制，IC

从34.91 μmol/L降为10.65 μmol/L (P0.05)。Cur-NPs明显提高了Cur诱导LLC细胞凋亡(P0.05)、抑制细胞周期于S期(P0.05)、升高细胞内钙离子浓度(P0.05)、升高细胞活性氧浓度(P0.05)、降低细胞线粒体膜电位(P0.05)的能力。结论新型姜黄素纳米粒可明显提高游离姜黄素对LLC细胞的体外增殖抑制作用，可能与其促进细胞凋亡有关。

Abstract

Objective To determine the effect of novel curcumin nanoparticles (Cur-NPs) on the proliferation and apoptosis of Lewis lung cancer (LLC) cells in vitro. Methods LLC cells were cultured with different concentrations of Cur-NPs and free curcumin (Cur) for 24 h

and the cell proliferation was evaluated by MTT assay. After 20 mol/L of Cur-NPs and Cur treatment on LLC cells for 24 h

the cell apoptosis

cell cycle arrest

reactive oxygen accumulation

intracellular Ca

accumulation and mitochondrial membrane potential were analyzed by flow cytometry.

Results Cur-NPs remarkably enhanced Cur-induced proliferation inhibition of LLC cells in vitro

and IC

decreased from 34.91 to 10.65 mol/L (P0.05). Cur-NPs significantly improved Cur-induced apoptosis (P0.05)

arrested cell cycle at S phase (P0.05)

increased reactive oxygen accumulation (P0.05) and intracellular Ca

accumulation (P0.05)

and decreased mitochondrial membrane potential (P0.05). Conclusion Cur-NPs can enhance Cur-induced proliferation inhibition in LLC cells

which may be related to promotion of cell apoptosis.

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College of Life Sciences， Beijing University of Chinese Medicine

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