Collagen Ⅱ promotes differentiation of rat bone marrow mesenchymal stem cells into chondrocytes in vitro

王冠; 陈星星; 薛鑫; 郭乔楠; 刘鹏; 范伟力; 刘明永; 赵建华

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Collagen Ⅱ promotes differentiation of rat bone marrow mesenchymal stem cells into chondrocytes in vitro

更新时间：2026-01-09

- Collagen Ⅱ promotes differentiation of rat bone marrow mesenchymal stem cells into chondrocytes in vitro
- Journal of Army Medical University Vol. 37, Issue 9, Pages: 886-890(2015)
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  CLC： R322.72;R329.2;R341.26
- Published：2015
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王冠,陈星星,薛鑫,郭乔楠,刘鹏,范伟力,刘明永,赵建华.Ⅱ型胶原体外促进大鼠骨髓间充质干细胞向软骨细胞分化[J].陆军军医大学学报(原第三军医大学学报),2015,37(09):886-890.

　Wang Guan,Chen Xingxing,Xue Xin,Guo Qiaonan,Liu Peng,Fan Weili,Liu Mingyong,Zhao Jianhua.Collagen Ⅱ promotes differentiation of rat bone marrow mesenchymal stem cells into chondrocytes in vitro[J].J Amry Med Univ (J Third Mil Med Univ),2015,37(09):886-890.
王冠,陈星星,薛鑫,郭乔楠,刘鹏,范伟力,刘明永,赵建华.Ⅱ型胶原体外促进大鼠骨髓间充质干细胞向软骨细胞分化[J].陆军军医大学学报(原第三军医大学学报),2015,37(09):886-890. DOI：

　Wang Guan,Chen Xingxing,Xue Xin,Guo Qiaonan,Liu Peng,Fan Weili,Liu Mingyong,Zhao Jianhua.Collagen Ⅱ promotes differentiation of rat bone marrow mesenchymal stem cells into chondrocytes in vitro[J].J Amry Med Univ (J Third Mil Med Univ),2015,37(09):886-890. DOI：

摘要

目的探讨Ⅱ型胶原（collagen Ⅱ）在体外促进大鼠骨髓间充质干细胞(bone mesenchymal stem cells

BMSCs)向软骨细胞的定向分化作用。方法体外培养BMSCs，分不同collagen Ⅱ接种浓度组(25、50、100、200 μg/mL)和空白对照组，分别作用于BMSCs 7、14、21 d，采用CCK-8法检测各不同浓度组对BMSCs活性的影响；qPCR法和免疫荧光法检测不同浓度组处理BMSCs后，各浓度组与空白对照组相比，collagen Ⅱ、SOX9及aggrecan mRNA及蛋白表达量的差异。结果 ①CCK-8检测结果显示各浓度组在7、14、21 d D（450）值差异无统计学意义（P0.05）；②qPCR检测结果显示21 d后，不同浓度组与空白对照组比较，collagenⅡ、SOX9及aggrecan mRNA表达量均升高（P0.05）

以100 μg/mL组最高(P0.01)；14 d时，各浓度组collagen Ⅱ、SOX9及aggrecan mRNA以100 μg/mL 组上调最明显（P0.05），而在7 d时，各组之间三者mRNA的表达差异无统计学意义（P0.05）；③各浓度组21 d时免疫荧光结果显示：在100 μg/mL Ⅱ组collagen Ⅱ、SOX9及aggrecan mRNA蛋白的表达量较其他各组均明显上调（P0.05）。结论 collagen Ⅱ可在体外促进大鼠BMSCs向软骨细胞分化，其中以100 μg/mL 组促进作用最为明显。

Abstract

Objective To investigate the promotion of type Ⅱ collagen (collagen Ⅱ) on differentiation of rat bone marrow mesenchymal stem cells (BMSCs) into chondrocytes. Methods BMSCs

seeded with different concentrations of type Ⅱ collagen (25

100 and 200 μg/mL) and control group

were assessed by CCK-8 assay on days 7

14 and 21. qPCR and immunofluorescence assay were used to detect the mRNA and protein expression of collagen Ⅱ

SOX9 and aggrecan in different intervention groups. Results CCK-8 assay showed that the cell viability had no significance on days 7

14 and 21 in different intervention groups (P0.05). The mRNA expression of collagen Ⅱ

SOX9 and aggrecan was up-regulated on day 21 (P0.05)

especially those in the 100 μg/mL intervention group most significant (P0.01)

while the mRNA expression in group of 100 μg/mL was highly up-regulated on day 14 (P0.05)

but there was no significance on day 7

compared with control group (P0.05). Immunofluorescence assay results showed that the protein levels of collagen Ⅱ

SOX9 and aggrecan were obviously enhanced in the 100 μg/mL intervention group than the other groups (P0.05). Conclusion Collagen Ⅱ promotes rat BMSCs differentiation into chondrocytes in vitro

with the dose of 100 μg/mL having the best efficiency.

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