IL-6 suppresses CCR7 expression in human monocyte-derived dendritic cells via STAT3 pathway

李腾飞; 苏崇宇; 黄刚; 付小龙; 余佩武; 赵永亮

您当前的位置：

首页 >

文章列表页 >

IL-6 suppresses CCR7 expression in human monocyte-derived dendritic cells via STAT3 pathway

更新时间：2026-01-09

- IL-6 suppresses CCR7 expression in human monocyte-derived dendritic cells via STAT3 pathway
- Journal of Army Medical University Vol. 37, Issue 8, Pages: 751-756(2015)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：
  CLC： R392.12;R392.32
- Published：2015
- 稿件说明：
移动端阅览
李腾飞,苏崇宇,黄刚,付小龙,余佩武,赵永亮.STAT3信号通路在IL-6抑制人单核细胞来源树突状细胞CCR7表达中的作用[J].陆军军医大学学报(原第三军医大学学报),2015,37(08 ):751-756.　Li Tengfei,Su Chongyu,Huang Gang,Fu Xiaolong,Yu Peiwu,Zhao Yongliang.IL-6 suppresses CCR7 expression in human monocyte-derived dendritic cells via STAT3 pathway[J].J Amry Med Univ (J Third Mil Med Univ),2015,37(08 ):751-756.

李腾飞, 苏崇宇, 黄刚, et al. IL-6 suppresses CCR7 expression in human monocyte-derived dendritic cells via STAT3 pathway[J]. Journal of Army Medical University, 2015, 37(8): 751-756.
李腾飞,苏崇宇,黄刚,付小龙,余佩武,赵永亮.STAT3信号通路在IL-6抑制人单核细胞来源树突状细胞CCR7表达中的作用[J].陆军军医大学学报(原第三军医大学学报),2015,37(08 ):751-756.　Li Tengfei,Su Chongyu,Huang Gang,Fu Xiaolong,Yu Peiwu,Zhao Yongliang.IL-6 suppresses CCR7 expression in human monocyte-derived dendritic cells via STAT3 pathway[J].J Amry Med Univ (J Third Mil Med Univ),2015,37(08 ):751-756. DOI：

李腾飞, 苏崇宇, 黄刚, et al. IL-6 suppresses CCR7 expression in human monocyte-derived dendritic cells via STAT3 pathway[J]. Journal of Army Medical University, 2015, 37(8): 751-756. DOI：

摘要

目的探讨STAT3信号通路在IL-6抑制人单核细胞来源树突状细胞（human monocyte-derived dendritic cells

moDCs）趋化因子受体7(C-C chemokine receptor type 7

CCR7)表达中的作用。方法采用CD14

磁珠分离法从外周血中分离得到单核细胞，加入rhGM-CSF和rhIL-4在体外诱导培养树突状细胞。LPS用于刺激moDCs表达CCR7，RT-PCR检测不同浓度IL-6处理后再用LPS刺激后的moDCs CCR7 mRNA表达。Western blot检测IL-6处理moDCs和IL-6预处理后再加入STAT3磷酸化特各异性抑制剂JSI-124的moDCs STAT3 、p-STAT3表达。IL-6预处理并抑制STAT3磷酸化后，LPS刺激48 h，RT-PCR检测moDCs CCR7 mRNA的表达，流式细胞仪检测moDCs CCR7蛋白表达，细胞迁移实验检测moDCs细胞迁移功能。结果与单独LPS刺激组比较，IL-6明显抑制LPS刺激的moDCs CCR7 mRNA的表达（P0.05）。Western blot 结果显示，与单独LPS刺激组比较，IL-6导致moDCs细胞内STAT3高度活化；而抑制STAT3信号高度活化后，与IL-6+LPS处理组比较，moDCs CCR7 mRNA表达、蛋白分子表达、细胞迁移能力明显升高（P0.05）

与LPS刺激组无差异（P0.05）。结论 IL-6通过高度活化STAT3信号通路抑制人单核细胞来源树突状细胞CCR7表达，导致树突状细胞的迁移能力受损，而靶向干预STAT3通路可能成为纠正树突状细胞迁移障碍的潜在手段。

Abstract

Objective To study the role of signal transducer and activator of transcription 3 (STAT3) signaling pathway in interleukin (IL)-6-induced C-C chemokine receptor type 7 (CCR7) suppression in human monocyte-derived dendritic cells (moDCs). Methods To obtain human moDCs

monocytes were isolated from venous blood of healthy donor by magnetic beads coated with anti-CD14 antibody

and cultured in complete culture medium containing rhGM-CSF and recombinant human IL-4. Lipopolysaccharide (LPS) was added into the culture medium of moDCs to induce the CCR7 expression. The expression of CCR7 mRNA in the moDCs treated with different concentrations of IL-6 and then LPS was analyzed by real-time PCR. The expression of STAT3 and p-STAT3 in moDCs treated with IL-6 and moDCs treated with IL-6 and JSI-124 (selective inhibitor of STAT3 pathway) were detected by Western blotting. The expressions of CCR7 mRNA and protein in the moDCs treated with IL-6

JSI-124 and LPS were analyzed by real-time PCR and flow cytometry

respectively. The migration ability of moDCs was analyzed by Trasnwell migration experiment. Results Compared with the moDCs incubated with LPS only

the moDCs cultured in the presence of IL-6 expressed lower levels of CCR7 mRNA (P0.05). The expression level of p-STAT3 in the moDCs treated with LPS and IL-6 was much higher than that in the moDCs treated with LPS only. JSI-124 could significantly inhibit STAT3 hyperactivity caused by IL-6

and after STAT3 hyperactivity was inhibited with JSI-124

the CCR7 expression and migration ability of the moDCs were increased (P0.05). Conclusion IL-6-STAT3 pathway is involved in IL-6-induced CCR7 suppression of human moDCs

and the intervention of STAT3 pathway may be potential means for repairing the damaged cell migration of dendritic cells.

关键词

Keywords

references

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Protective effects of lycopene on intestinal ischemia-reperfusion injury in rats

Clinical significance of serum interleukin-22 level in patients with acute HEV infection

Serum levels of high mobility group box-1 and interleukin-6 in cerebral infarction patients and their correlation with the disease’s risk factors

Value of risk stratification of coronary heart disease by combined determination of myeloperoxidase, interleukin-6 and high sensitivity C-reactive protein

Relationship of interleukin-6 gene polymorphisms and its serum level with essential hypertension

Related Author

甘嘉亮

李通

谭杨波

何登明

朱鹏

朱季香

徐城

郭世民

Related Institution

No data

⁰