Expression profiles of T cell factor 3 in non-small cell lung cancer tissues

闵利; 刘煜亮; 徐瑜; 李万成; 陈虹

您当前的位置：

首页 >

文章列表页 >

Expression profiles of T cell factor 3 in non-small cell lung cancer tissues

更新时间：2026-01-09

- Expression profiles of T cell factor 3 in non-small cell lung cancer tissues
- Journal of Army Medical University Vol. 37, Issue 5, Pages: 425-428(2015)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：
  CLC： R394.3;R730.23;R734.2
- Published：2015
- 稿件说明：
移动端阅览
闵利,刘煜亮,徐瑜,李万成,陈虹.T细胞因子3在非小细胞肺癌中的表达[J].陆军军医大学学报(原第三军医大学学报),2015,37(05):425-428.

　Min Li,Liu Yuliang,Xu Yu,Li Wancheng,Chen Hong.Expression profiles of T cell factor 3 in non-small cell lung cancer tissues[J].J Amry Med Univ (J Third Mil Med Univ),2015,37(05):425-428.
闵利,刘煜亮,徐瑜,李万成,陈虹.T细胞因子3在非小细胞肺癌中的表达[J].陆军军医大学学报(原第三军医大学学报),2015,37(05):425-428. DOI：

　Min Li,Liu Yuliang,Xu Yu,Li Wancheng,Chen Hong.Expression profiles of T cell factor 3 in non-small cell lung cancer tissues[J].J Amry Med Univ (J Third Mil Med Univ),2015,37(05):425-428. DOI：

摘要

目的研究T细胞因子3（T cell factor 3

TCF-3）在非小细胞肺癌中的表达及分布情况。方法采用50对人非小细胞肺癌组织标本及其正常癌旁组织对照，进行免疫组化检测TCF-3蛋白在肺癌组织及正常肺组织中的表达及分布情况；采用5种人非小细胞肺癌细胞系及2种正常人支气管上皮细胞系进行实时荧光定量PCR，Western blot 检测肺癌细胞系中TCF-3在mRNA和蛋白水平的表达情况。结果 (1) TCF-3在肺癌组织中表达，表达率为48%（24/50），在正常肺组织中未见表达。TCF-3在肺癌细胞胞核与胞质同表达，以细胞核表达为主。(2)TCF-3在A549、H520、HBE、BEP-2D 4种细胞系中mRNA及蛋白水平均有表达。结论 TCF-3在肺癌组织及细胞系中均表达，TCF-3蛋白在细胞核与细胞质同表达，但以细胞核表达为主。

Abstract

Objective To determine the expression profiles and distribution of T cell factor-3 (TCF-3) in non-small cell lung cancer (NSCLC). Methods TCF-3 expression was examined in 50 NSCLC tissue specimens and their corresponding normal lung specimens by immunohistochemical assay (S-P method). The mRNA levels of TCF-3 were detected in 5 cell lines of NSCLC (A549

H460

H226

H520 and SK-MES-1 cell lines ) and 2 human normal bronchial epithelial cell lines (HBE and BEP-2D cell lines) by real-time fluorescence quantitative PCR. The protein level of TCF-3 gene in the cell lines was detected by Western blotting. Results Among the 50 lung cancer specimens

24 samples showed high expression of TCF-3 (48%)

and 22 samples had the expression both in the cytoplasm and nuclei. But no TCF-3 expression was detected in 50 corresponding normal lung specimens. The expression of TCF-3 was found at mRNA and protein levels in the cell lines A549

H520

HBE

and BEP-2D. Conclusion TCF-3 is expressed in the NSCLC tissue as well as cell lines. TCF-3 protein is located in the nuclei and cytoplasm

but mainly in the former.

关键词

Keywords

references

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Integrative model combining deep learning, clinical and radiomic features enhances EGFR mutation prediction in non-small cell lung cancer

Preparation of doxorubicin-loaded polyphyllin H liposomes and synergistic anti-tumor activity against non-small cell lung cancer in vitro

Development of a postoperative recurrence prediction model for stage Ⅰ non-small cell lung cancer patients using multimodal data based on machine learning

Effect of chronic obstructive pulmonary disease on immunotherapy in patients with advanced non-small cell lung cancer

Efficacy and resistance of EGFR-TKIs for first-line treatment for compound EGFR mutations positive non-small cell lung cancer

Related Author

YANG Taotao

CHEN Wei

CHEN Cancan

YAN Wanying

WANG Dawei

XIONG Kunlin

SUN Zhiyuan

WANG Xianqi

Related Institution

Department of Radiology，First Affiliated Hospital， Army Medical University （Third Military Medical University）

Infervision Medical Technology Co.， Ltd

Department of Radiology， Daping Hospital， Army Medical University

Department of Diagnostic Radiology， Eastern Theatre General Hospital

Department of Medical Service,No. Hospital of Joint Logistics Support Force

⁰