miR-125b regulates self-renewal in liver cancer stem cells

彭维; 赵湘湘; 苏立; 钱程

您当前的位置：

首页 >

文章列表页 >

miR-125b regulates self-renewal in liver cancer stem cells

更新时间：2026-01-09

- miR-125b regulates self-renewal in liver cancer stem cells
- Journal of Army Medical University Vol. 37, Issue 11, Pages: 1070-1074(2015)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：
  CLC： R73-354;R730.23;R735.7
- Published：2015
- 稿件说明：
移动端阅览
彭维,赵湘湘,苏立,钱程.miR-125b对肝癌干细胞自我更新能力的调控作用[J].陆军军医大学学报(原第三军医大学学报),2015,37(11):1070-1074.

　Peng Wei,Zhao Xiangxiang,Su Li,Qian Cheng.miR-125b regulates self-renewal in liver cancer stem cells[J].J Amry Med Univ (J Third Mil Med Univ),2015,37(11):1070-1074.
彭维,赵湘湘,苏立,钱程.miR-125b对肝癌干细胞自我更新能力的调控作用[J].陆军军医大学学报(原第三军医大学学报),2015,37(11):1070-1074. DOI：

　Peng Wei,Zhao Xiangxiang,Su Li,Qian Cheng.miR-125b regulates self-renewal in liver cancer stem cells[J].J Amry Med Univ (J Third Mil Med Univ),2015,37(11):1070-1074. DOI：

摘要

目的观察miR-125b对肝癌干细胞（liver cancer stem cells，LCSCs）成球、克隆形成、增殖、凋亡、迁移的作用，探讨miR-125b对肝癌干细胞自我更新能力的调控作用。方法获取肝癌干细胞；包装表达miR-125b的慢病毒并感染肝癌干细胞；光镜计数法检测miR-125b对肝癌干细胞成球率及克隆形成率的影响；CCK-8法检测miR-125b对肝癌干细胞增殖能力的影响；DAPI染色观察miR-125b对肝癌干细胞凋亡的影响；Transwell实验检测miR-125b对肝癌干细胞迁移能力的影响。结果与对照组比较，miR-125b显著降低LCSCs的成球率（对照组27.7%，实验组0.0%，P0.01）以及抑制其克隆形成能力（对照组29%，实验组6%，P0.01）；同时miR-125b可促进LCSCs的凋亡，抑制LCSCs的迁移能力（P0.01）。结论 miR-125b对LCSCs的成球能力、克隆形成能力具有明显的抑制作用，表明miR-125b对LCSCs的自我更新能力具有负向调控作用。miR-125b也能抑制LCSCs的迁移，对LCSCs的凋亡具有促进作用。

Abstract

Objective To determine the effects of miR-125b on the sphere formation

clone formation

proliferation

apoptosis and migration of liver cancer stem cells (LCSCs)

and investigate the regulative role of miR-125b on the self-renewal capacity of LCSCs. Methods The lentivirus expressing miR-125b was used to infect obtained LCSCs. Light microscope counting was employed to measure the sphere formation and clone formation in the infected LCSCs. CCK-8 assay was used to detect the effect of miR-125b on the proliferation of LCSCs. DAPI staining and transwell chamber test was used to observe the effect of miR-125b on the cell apoptosis and migration. Results Compared with the control cells

miR-125b significant decreased sphere formation (0.0% vs 27.7%

P0.01)

inhibited the colony formation (6% vs 29%

P0.01)

promoted the cell apoptosis

and inhibited the migration ability in LCSCs after lentivirus infection (P0.01). Conclusion miR-125b exerts significant inhibitory effect on sphere and clone formation of LCSCs

indicating its negative role in the regulation of self-renewal capacity of the stem cells. It also can inhibit the migration and promote the apoptosis of LCSCs.

关键词

Keywords

references

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Effect of RNA interference-mediated S100A9 gene silencing on proliferation and self-renewal of U87 glioma stem cells in vitro

IGF-FOXO3a pathway regulates self-renewal of liver cancer stem cells in vitro

COMMD7 inhibits migration and invasion in liver cancer stem cells via regulating mesenchymalepithelial transition

Knockdown of scinderin attenuates self-renewal capacity in gastric cancer stem cells

Low concentration of sodium arsenite induces differentiation of liver cancer stem cells through inhibiting Oct4 and Sox2 expression in hepatocellular carcinoma cells

Related Author

霍钢

陈松

陈宸

陈军

单娟娟

沈俊杰

李志芬

吴柯

Related Institution

No data

⁰