Effect of miRNA-29b on regulating hypertrophy of bone marrow mesenchymal stem cell-derived chondrocytes

苗莹; 赵春容; 康菲; 闫演飞; 董世武

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Effect of miRNA-29b on regulating hypertrophy of bone marrow mesenchymal stem cell-derived chondrocytes

更新时间：2026-01-09

- Effect of miRNA-29b on regulating hypertrophy of bone marrow mesenchymal stem cell-derived chondrocytes
- Journal of Army Medical University Vol. 37, Issue 10, Pages: 952-956(2015)
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  CLC： R322.71;R329.21;R329.23
- Published：2015
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苗莹,赵春容,康菲,闫演飞,董世武.miRNA-29b调控骨髓间充质干细胞来源的软骨细胞肥大化的作用研究[J].陆军军医大学学报(原第三军医大学学报),2015,37(10):952-956.

　Miao Ying,Zhao Chunrong,Kang Fei,Yan Yanfei,Dong Shiwu.Effect of miRNA-29b on regulating hypertrophy of bone marrow mesenchymal stem cell-derived chondrocytes[J].J Amry Med Univ (J Third Mil Med Univ),2015,37(10):952-956.
苗莹,赵春容,康菲,闫演飞,董世武.miRNA-29b调控骨髓间充质干细胞来源的软骨细胞肥大化的作用研究[J].陆军军医大学学报(原第三军医大学学报),2015,37(10):952-956. DOI：

　Miao Ying,Zhao Chunrong,Kang Fei,Yan Yanfei,Dong Shiwu.Effect of miRNA-29b on regulating hypertrophy of bone marrow mesenchymal stem cell-derived chondrocytes[J].J Amry Med Univ (J Third Mil Med Univ),2015,37(10):952-956. DOI：

摘要

目的探讨miR-29b在骨髓间充质干细胞（bone marrow mesenchymal stem cells，BMSCs）分化为软骨细胞时，对细胞肥大化进程的调控作用。方法培养BMSCs并进行软骨细胞肥大化方向的诱导，检测软骨细胞标志基因Ⅱ型胶原（Col Ⅱ）、肥大化细胞标志基因Ⅹ型胶原（Col Ⅹ和Runx2），并用特异性染色法确定细胞肥大化诱导成功，再检测miR-29b在转录水平的表达情况。然后将实验分为miR-29b过表达（miR-29b mimics）和miR-29b抑制（miR-29b抑制剂）两组，每组分别设置空白对照组，分别检测两处理组中上述软骨细胞和肥大化细胞相关标志的表达变化。结果 miR-29b过表达组中，Col Ⅱ表达与对照组相比显著降低（P0.05），Col Ⅹ和Runx2表达显著升高（P0.05）；而miR-29b抑制组中，Col Ⅱ的表达与对照组相比显著升高（P0.05），Col Ⅹ和Runx2显著降低（P0.05）。结论 miR-29可以有效促进BMSCs向肥大化的软骨细胞分化，而抑制miR-29b可降低BMSCs来源的软骨细胞的肥大化。

Abstract

Objective To investigate the effect of miR-29b on regulating cell hypertrophy in the differentiation of bone marrow mesenchymal stem cells (BMSCs) into chondrocytes. Methods BMSCs were cultured and induced into hypertrophic chondrocytes. Collagen type Ⅱ (Col Ⅱ

marker of chondrocytes) and collagen type X (Col X

marker of hypertrophic chondrocytes) were detected by qRT-PCR. Safranin O staining was employed to confirm the hypertrophic state of the chondrocytes

and then the expression of miR-29b in the transcriptional level was detected by qRT-PCR. In the experiment

the BMSCs were divided into the following groups: miR-29b over-expression group

miR-29b inhibition group

over-expression negative control group and inhibition negative control group. The expression of the related markers in the 4 groups were detected. Results In the miR-29b mimics group

the expression of Col Ⅱ was decreased significantly compared with the control group (P0.05)

while the expression of Col X and Runx2 was significantly increased (P0.05). However

the expression of Col Ⅱ in the miR-29b inhibition group was significantly higher than that in the control group (P0.05)

while that of Col X and Runx2 was significantly reduced (P0.05). Conclusion MiR-29 can effectively promote hypertrophy of BMSC-derived chondrocytes

and the inhibition of miR-29 can stabilize the phenotype of BMSC-derived chondrocytes.

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