Screening and identification of HLA-DRB1<sup>*</sup>0803 and HLA-DRB1<sup>*</sup>0901 restricted epitopes from hepatitis B core antigen

李辉文; 何韦韦; 谭文婷; 王莉; 陈晓玲; 邓国宏

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Screening and identification of HLA-DRB1^*0803 and HLA-DRB1^*0901 restricted epitopes from hepatitis B core antigen

更新时间：2026-01-09

- Screening and identification of HLA-DRB1^*0803 and HLA-DRB1^*0901 restricted epitopes from hepatitis B core antigen
- Journal of Army Medical University Vol. 37, Issue 9, Pages: 833-838(2015)
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  CLC： R341;R392-3;{R392.13}
- Published：2015
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李辉文,何韦韦,谭文婷,王莉,陈晓玲,邓国宏.HLA-DRB1*0803与HLA-DRB1*0901等位特异性HBcAg表位肽的筛查与鉴定[J].陆军军医大学学报(原第三军医大学学报),2015,37(09):833-838.

　Li Huiwen,He Weiwei,Tan Wenting,Wang Li,Chen Xiaoling,Deng Guohong.Screening and identification of HLA-DRB1*0803 and HLA-DRB1*0901 restricted epitopes from hepatitis B core antigen[J].J Amry Med Univ (J Third Mil Med Univ),2015,37(09):833-838.
李辉文,何韦韦,谭文婷,王莉,陈晓玲,邓国宏.HLA-DRB1*0803与HLA-DRB1*0901等位特异性HBcAg表位肽的筛查与鉴定[J].陆军军医大学学报(原第三军医大学学报),2015,37(09):833-838. DOI：

　Li Huiwen,He Weiwei,Tan Wenting,Wang Li,Chen Xiaoling,Deng Guohong.Screening and identification of HLA-DRB1*0803 and HLA-DRB1*0901 restricted epitopes from hepatitis B core antigen[J].J Amry Med Univ (J Third Mil Med Univ),2015,37(09):833-838. DOI：

摘要

目的鉴定HLA-DRB1

0803与HLA-DRB1

0901等位特异性HBV表位，进一步认识HLA-DR不同等位限制性表位的差异。方法 BLCL细胞表面高表达HLA-DR分子，从HBcAg肽池负载的特征HLA-DR等位的BLCL细胞表面上收集相关多肽，多肽经过过滤、脱盐、浓缩等处理步骤后对其进行液质联用质谱分析（LC-MS），再结合生物信息学预测工具NetMHCⅡpan 3.0 Server初步确定其潜在候选表位。结果筛选出HLA-DRB1

0803限制性表位HBc17-31、HBc22-36、HBc27-41、HBc82-96、HBc87-101、HBc92-106、HBc117-131、HBc122-136共8条，HLA-DRB1

0901限制性表位HBc2-16、HBc7-21、HBc12-26、HBc27-41、HBc82-96、HBc87-101、HBc122-136共7条，其中相同的有4条。结论发现了HBc7-21、HBc22-36、HBc27-41、HBc82-96、HBc87-101、HBc92-106新的HLA-Ⅱ类分子表位，不同的HLA-DR等位限制的HBcAg表位肽存在差异。

Abstract

Objective To identify the specific epitopes of HLA-DRB1

0803 and HLA-DRB1

0901 alleles from hepatitis B virus for further understanding the difference of the epitopes restricted to the different HLA-DR alleles. Methods After incubating the peptide pool of pre-core and core sequence of hepatitis B virus with a B lymphoblastoid cell line (BLCL

homozygous human B cell lines of allele

HLA-DRB1

0803 or HLA-DRB1

0901 with cell surface highly expressing HLA-DR molecules)

the peptides were eluted and collected from the BLCL surface without affecting cell viability. Then the peptides were filtered

desalted

concentrated and analyzed by liquid chromatography-mass spectrometry (LC-MS). Bioinformatics predictive tool NetMHCⅡpan 3.0 Server was employed to confirm the candidate epitopes. Results There were 8 potential epitopes restricted to HLA-DRB1

0803 finally identify from HBV

that is

HBc17-31

HBc22-36

HBc27-41

HBc82-96

HBc87-101

HBc92-106

HBc117-131

and HBc122-136

and 7 potential epitopes restricted to HLA-DRB1

0901

that is

HBc2-16

HBc7-21

HBc12-26

HBc27-41

HBc82-96

HBc87-101

and HBc122-136. Four of them were same epitopes. Conclusion We have found 6 new epitopes to HLA class Ⅱ

HBc7-21

HBc22-36

HBc27-41

HBc82-96

HBc87-101 and HBc92-106

and there are some differences among the HBcAg epitopes restricted to different HLA-DR alleles.

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Screening and identification of HLA-DRB1*0803 and HLA-DRB1*0901 restricted epitopes from hepatitis B core antigen

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摘要

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关键词

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Screening and identification of HLA-DRB1^0803 and HLA-DRB1^0901 restricted epitopes from hepatitis B core antigen