Objective      To investigate the alterations of intestinal microflora community  diversity in newborns with feeding intolerance (FI) and the relationship with disease outcome.       Methods      Using 16s rDNA PCR-DGGE

we assessed 12 FI newborns’ bacterial diversity in stool specimens within 24 h of the symptom onset (t

0

) and after the recovery of FI (t

1

). T-A cloning kit and sequencing were used to investigate the distribution

of bacteria and common dominant bacteria in stool specimens. At the same time

we collected the stool specimens of 12 feeding tolerance newborns with the same gestational age

birth weight and day-old as control.       Results      In contrast to the control group

both the bands (S) of intestinal bacterial and the Shanon-wiener indexes (H) were relatively lower in the FI group at t

0

(9.00±2.83 vs 12.76±2.38

2.12±0.36 vs 2.51±0.19

P0.05). However

there was no statistical significant difference in S and H between the FI group at t

1

and the control group (14.00±1.91 vs 14.75±1.76

2.61±0.14 vs 2.66±0.13

P0.05). Compared with the control group

the results of molecular cloning and sequencing showed that the proportion of Klebsiella was significantly higher in the FI group (53.40% vs 40.94%

P0.05).       Conclusion      There is a decrease in intestinal microflora community diversity in newborns with FI

which is recovered with the rehabilitation of this disease. Higher proportion of Klebsiella in intestinal microflora might be correlated with the onset of FI in newborns.