Antimicrobial resistance and  serotype   of Haemophilus influenzae isolates from Chongqing children during 2009 to 2013

景春梅; 王偲

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Antimicrobial resistance and serotype of Haemophilus influenzae isolates from Chongqing children during 2009 to 2013

更新时间：2026-01-09

- Antimicrobial resistance and serotype of Haemophilus influenzae isolates from Chongqing children during 2009 to 2013
- Journal of Army Medical University Vol. 36, Issue 23, Pages: 2400-2403(2014)
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  CLC： R181.36;R373.13;R915
- Published：2014
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景春梅,王偲.2009-2013年重庆地区儿童感染流感嗜血杆菌的耐药性及血清型分析[J].陆军军医大学学报(原第三军医大学学报),2014,36(23):2400-2403.

　Jing Chunmei,Wang Cai.Antimicrobial resistance and serotype of Haemophilus influenzae isolates from Chongqing children during 2009 to 2013[J].J Amry Med Univ (J Third Mil Med Univ),2014,36(23):2400-2403.
景春梅,王偲.2009-2013年重庆地区儿童感染流感嗜血杆菌的耐药性及血清型分析[J].陆军军医大学学报(原第三军医大学学报),2014,36(23):2400-2403. DOI：

　Jing Chunmei,Wang Cai.Antimicrobial resistance and serotype of Haemophilus influenzae isolates from Chongqing children during 2009 to 2013[J].J Amry Med Univ (J Third Mil Med Univ),2014,36(23):2400-2403. DOI：

摘要

目的统计分析2009-2013年重庆地区儿童感染流感嗜血杆菌(Haemophilus influenzae，Hi)的耐药性及血清型，为临床治疗Hi感染和推广Hi疫苗接种提供参考依据。方法纸片扩散法检测Hi的耐药性，玻片凝集法对Hi进行血清分型。结果共分离Hi 5 474株，以呼吸道标本多见，占97.4%(5 329株) 。6个月～3岁组和3～5岁组的Hi分离率分别为21.8%和16.3%，高于6个月组和5岁组的分离率10.3%和6.6%，82.2%的分离株在≤5岁年龄段；复方新诺明、氨苄西林、头孢噻肟、氨苄西林/舒巴坦、左氧氟沙星的耐药率分别为77.0%、42.4%、0.2 %、2.3%、0.4%。2009-2013年Hi对复方新诺明、阿莫西林/棒酸、氨苄西林、氯霉素、利福平、头孢呋辛、阿奇霉素耐药率有明显改变（P0.05），其余受试药物的耐药率均无明显改变（P0.05）。共分离出33株β-内酰胺酶阴性氨苄西林耐药Hi，占0.6%。2282株Hi产β-内酰胺酶，产酶率为41.7%。不可分型Hi占92.8%，可分型Hi中a、b、c型各占5.6%，d、e、f型分别为27.8%、38.9%、16.7%。结论 ≤5岁儿童是Hi感染易感人群，Hi主要分离自呼吸道标本，复方新诺明已不宜用于Hi感染的经验用药，临床应重视Hi的耐药现象，并加强Hi对抗菌药物的耐药性监测。血清分型以不可分型株为主，Hie是重庆地区儿童Hi的主要血清型。

Abstract

Objective To investigate the antimicrobial resistance and serotype of Haemophilus influenzae (Hi) isolates from the children in Chongqing during the year of 2009 to 2013

and provide instruction for prevention and pharmacological application. Methods Antimicrobial susceptibility testing was performed by Kirby-Bauer disc diffusion method

and serotypes were measured by Hi antiserum. Results A total of 5 474 Hi isolates were collected

97.4% (5 329 isolates) were from respiratory samples. All the samples were divided into 4 groups according to the children’s ages

that is

younger than 6 months (group 1)

6 months to 3 years (group 2)

3 years to 5 years (group 3) and older than 5 years (group 4). The positive rate in group 2 (21.8%) and group 3 (16.3%) were significantly higher than that in group 1 (10.3%) and group 4 (6.6%). Among all the 5 474 Hi strains

82.2% were identified from children younger than 5 years old. The resistant rate to trimethoprim-sulfamethoxazole

ampicillin

cefotaxime

ampicillin/sulbactam

levofloxacin was 77.0% 42.4%

0.2 %

2.3% and 0.4%

respectively. There were significant changes among the resistance rates of trimethoprim-sulfamethoxazole

amoxycillin-clavulanic acid

ampicillin

chloramphenicol

rifampicin

cefuroxime

and azithromycin during the investigation period (P0.05). However

the difference of resistance rates of the rest antibiotics had no statistically significance (P0.05). A total of 33 isolates (0.6%) were beta-lactamase negative ampicillin-resistant (BLNAR) Hi. Total 2 282 isolates (41.7%) produced beta-lactamase. Nontypeable Haemophilus influenzae (NTHi) accounted for 92.8% among all Hi strains

and Hia

Hib

Hic

Hid

Hie

and Hif accounted for 5.6%

5.6%

27.8%

38.9%

and 16.7% respectively among all serotype of Hi. Conclusion Children younger than 5 years old are sensitive population for Hi infection. Most of Hi are isolated from respiratory samples

and trimethoprim-sulfamethoxazole is not suitable for Hi infection. More attention should be paid to resistant Hi

and to survey of antimicrobial resistance in Hi infection. NTHi is the major serotype of Hi

and the Hie is the main serotype.

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Related Institution

Department of Anesthesiology Children’s Hospital of Chongqing Medical University， National Clinical Research Center for Child Health and Disorders， Ministry of Education Key Laboratory of Child Development and Disorders， Chongqing Key Laboratory of Child Neurodevelopment and Cognitive Disorders

Department of Ophthalmology， First Affiliated Hospital/Southwest Hospital， Army Medical University （Third Military Medical University）

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