HGF promotes osteogenic differentiation in mesenchymal stem cells induced by bone morphogenetic protein 9

惠慧; 罗聪; 毕杨

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HGF promotes osteogenic differentiation in mesenchymal stem cells induced by bone morphogenetic protein 9

更新时间：2026-01-09

- HGF promotes osteogenic differentiation in mesenchymal stem cells induced by bone morphogenetic protein 9
- Journal of Army Medical University Vol. 36, Issue 23, Pages: 2380-2384(2014)
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  CLC： R322.47;R322.71;R329.2
- Published：2014
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惠慧,罗聪,毕杨.肝细胞生长因子促进骨形态发生蛋白9诱导小鼠骨髓间充质干细胞C3H10成骨分化的研究[J].陆军军医大学学报(原第三军医大学学报),2014,36(23):2380-2384.

　Hui Hui,Luo Cong,Bi Yang.HGF promotes osteogenic differentiation in mesenchymal stem cells induced by bone morphogenetic protein 9[J].J Amry Med Univ (J Third Mil Med Univ),2014,36(23):2380-2384.
惠慧,罗聪,毕杨.肝细胞生长因子促进骨形态发生蛋白9诱导小鼠骨髓间充质干细胞C3H10成骨分化的研究[J].陆军军医大学学报(原第三军医大学学报),2014,36(23):2380-2384. DOI：

　Hui Hui,Luo Cong,Bi Yang.HGF promotes osteogenic differentiation in mesenchymal stem cells induced by bone morphogenetic protein 9[J].J Amry Med Univ (J Third Mil Med Univ),2014,36(23):2380-2384. DOI：

摘要

目的探讨肝细胞生长因子（hepatocyte growth factor，HGF）联合骨形态发生蛋白9（bone morphogenetic protein 9，BMP9）诱导小鼠骨髓间充质干细胞（C3H10）成骨分化的影响，并探讨其可能的分子机制。方法用重组腺病毒介导BMP9在C3H10细胞中过表达，同时联用20 ng/mL肝细胞生长因子HGF诱导C3H10细胞，Real-time PCR检测C3H10成骨分化相关基因骨钙蛋白（osteocalcin，OC）及骨桥蛋白（osteopontin，OPN）基因mRNA水平的表达变化；Western blot检测OC、OPN的蛋白表达水平；ALP染色及ALP读数分析C3H10细胞碱性磷酸酶（alkaline phosphatase，ALP）活性的变化；茜素红S染色检测细胞的钙盐沉积；Real-time PCR检测经典信号通路Smad1/5/8在此诱导过程中的表达水平。 结果 BMP9单独作用能诱导C3H10细胞向成骨方向分化，并增强细胞ALP活性，单独HGF有较弱的成骨诱导作用，但与BMP9联用可显著增强C3H10细胞成骨特异性标志物OC与OPN的mRNA及蛋白表达水平（P0.05），促进ALP活性，及晚期的钙盐沉积；HGF能刺激Smad1/5/8信号通路mRNA表达水平显著增高(P0.05)。结论肝细胞生长因子可显著增强BMP9诱导C3H10细胞成骨分化的作用，可能是通过激活Smad经典信号通路来实现的。

Abstract

Objective To determine the effect of hepatocyte growth factor (HGF) combined with bone morphogenetic protein 9 (BMP9) on the induced osteogenic differentiation of mesenchymal stem cell (MSC) line C3H10 and investigate the possible underlying mechanism. Methods C3H10 cells were treated by recombinant adenovirus expressing Ad-BMP9 infection and HGF (20 ng/mL) induction. The expression of osteocalcin (OC) and osteopontin (OPN) at mRNA and protein levels were determined by real-time PCR and Western blot analysis respectively. The activity of alkaline phosphatase (ALP) was detected by ALP staining and activity assay. Calcium deposition was detected by Alizarin Red S staining. At last

the mRNA expression of Smad1/5/8 was analyzed by real-time PCR. Results BMP9 alone induced the osteogenic differentiation of C3H10 cells and increased the ALP activity. HGF alone slightly induced osteogenic differentiation

while significantly promoted the BMP9-induced OC and OPN expression (P0.05)

ALP activity

and calcium deposition. Furthermore

HGF induction resulted in significant increase in the transcription levels of Smad1/5/8 (P0.05). Conclusion HGF synergistically promotes the effect of BMP9 on induction of osteogenic differentiation of C3H10 cells

and this effect maybe due to the activation of Smad signaling pathways.

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