Emodin improves learning and memory abilities in rats after hypoxic brain injury

张艳超; 张新颜; 程敬民; 马原; 余思逊; 孙志勇; 顾建文

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Emodin improves learning and memory abilities in rats after hypoxic brain injury

更新时间：2026-01-09

- Emodin improves learning and memory abilities in rats after hypoxic brain injury
- Journal of Army Medical University Vol. 36, Issue 17, Pages: 1813-1816(2014)
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  CLC： R282.71;R338.64;R743.31
- Published：2014
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张艳超,张新颜,程敬民,马原,余思逊,孙志勇,顾建文.大黄素对缺氧性脑损伤大鼠学习记忆能力的影响[J].陆军军医大学学报(原第三军医大学学报),2014,36(17):1813-1816.

　Zhang Yanchao,Zhang Xinyan,Cheng Jingmin,Ma Yuan,Yu Sixun,Sun Zhiyong,Gu Jianwen.Emodin improves learning and memory abilities in rats after hypoxic brain injury[J].J Amry Med Univ (J Third Mil Med Univ),2014,36(17):1813-1816.
张艳超,张新颜,程敬民,马原,余思逊,孙志勇,顾建文.大黄素对缺氧性脑损伤大鼠学习记忆能力的影响[J].陆军军医大学学报(原第三军医大学学报),2014,36(17):1813-1816. DOI：

　Zhang Yanchao,Zhang Xinyan,Cheng Jingmin,Ma Yuan,Yu Sixun,Sun Zhiyong,Gu Jianwen.Emodin improves learning and memory abilities in rats after hypoxic brain injury[J].J Amry Med Univ (J Third Mil Med Univ),2014,36(17):1813-1816. DOI：

摘要

目的探讨大黄素对缺氧环境所致脑损伤后大鼠学习记忆功能的保护及对脑组织超氧化物歧化酶（superoxide dismutase

SOD）和丙二醛（malondialdehyde

MDA）的影响。方法雄性Wistar大鼠80只，分为对照组（n=16）、缺氧组（n=16）及大黄素干预组（n=48），利用低氧动物实验舱模拟缺氧环境，舱内采用自制游泳箱强迫大鼠游泳运动，将缺氧组和各大黄素干预组大鼠置于舱内饲养，每3小时游泳50 min，持续24 h，对照组大鼠不进行缺氧处理。大黄素干预组分为15、30、60 mg/（kg·d）3个剂量组（n=16），于进舱前3 d按相应剂量行大黄素灌胃，对照组和缺氧组大鼠于相应时间点灌注生理盐水1 mL。利用Morris水迷宫图像自动采集处理系统对大鼠进行寻找隐藏平台的平均逃避潜伏期测试；采用干/湿质量法测定脑组织含水量的变化；观察大黄素对缺氧性脑损伤后大鼠脑组织SOD活性和MDA含量的影响。结果 Morris水迷宫实验中，缺氧组与对照组比较，平均逃避潜伏期升高（P0.01），各剂量大黄素干预组平均逃避潜伏期较缺氧组减少（P0.05，P0.01）；干/湿质量法测定各组脑组织含水量变化，缺氧组大鼠脑组织含水量较对照组显著增加（P0.01），各大黄素干预组与缺氧组相比，脑组织含水量减低（P0.05，P0.01），且呈剂量关系；脑组织SOD活性及MDA含量测定结果显示，缺氧组与对照组相比，SOD活性降低（P0.01），MDA含量增高（P0.01），各剂量大黄素干预组与缺氧组相比，SOD活性明显增高（P0.05，P0.01），MDA含量减少（P0.05，P0.01）。结论大黄素可以提高缺氧环境下大鼠脑组织 SOD活性

降低 MDA含量，改善大鼠学习记忆能力。

Abstract

Objective To determine the effects of emodin on the protection of learning and memory abilities and on SOD activity and MDA content in the brain tissue of rats after hypoxic brain injury induced by hypobaric hypoxia environment. Methods A total of 80 male Wistar rats were randomly divided into control group (n=16)

hypoxia group (n=16) and emodin intervention group (n=48). Hypoxia environment cabin was used to contain the rats of the 2 latter groups and force them to swim 50 min every 3 h for consecutive 24 hours. The rats of control group were feed in normal condition. The latter group was further assigned into 3 subgroups according to different doses of emodin [15

30 and 60 mg/(kg·d)

n=16]

and was perfused with corresponding doses intragastrically for 3 consecutive days before entering the hypoxia cabin

while the rats of the other 2 groups were given with same volume of normal saline (1 mL). Morris water maze automatic image acquisition and processing system was used to find the mean escape latency (MEL) for hidden platform. Brain water content was measured by wet to dry mass ratio method. SOD activity and MDA content in the cerebral tissue were detected. Results In the Morris water maze test

MEL was significantly longer in the hypoxia group than the control (P0.01)

and was obviously shorter in the emodin intervention groups than the hypoxia group (P0.05

P0.01); Brain water content was significantly increased in the hypoxia than the control (P0.01)

but the emodin intervention group had lower water content than the hypoxia group (P0.05

P0.01) in a dose-dependent manner. In the determination of the activity of brain tissue SOD and the content of MDA

the activity of SOD was significantly lower (P0.01) while MDA content was increased (P0.01) in hypoxia group than the control. Emodin treatment resulted in increased SOD activity (P0.05

P0.01) and decreased MDA content (P0.05

P0.01) than in the hypoxia group. Conclusion Emodin can increase the activity of SOD in brain tissue of rats

decrease the content of MDA

and improve the ability of learning and memory in rats.

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