Knockdown of PLCε gene inhibits proliferation in human 786-0 renal cancer cells via NF-κB/p65 pathway

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Knockdown of PLCε gene inhibits proliferation in human 786-0 renal cancer cells via NF-κB/p65 pathway

更新时间：2026-01-09

- Knockdown of PLCε gene inhibits proliferation in human 786-0 renal cancer cells via NF-κB/p65 pathway
- Journal of Army Medical University Vol. 36, Issue 19, Pages: 2010-2015(2014)
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  CLC： R394.3;R730.23;R737.11
- Published：2014
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杨雪,欧俐苹,王晓荣,王胤,吴小候,罗春丽.PLCε基因通过NF-κB/p65途径抑制肾癌786-0细胞的增殖[J].陆军军医大学学报(原第三军医大学学报),2014,36(19):2010-2015.

　Yang Xue,Ou Liping,Wang Xiaorong,Wang Yin,Wu Xiaohou,Luo Chunli.Knockdown of PLCε gene inhibits proliferation in human 786-0 renal cancer cells via NF-κB/p65 pathway[J].J Amry Med Univ (J Third Mil Med Univ),2014,36(19):2010-2015.
杨雪,欧俐苹,王晓荣,王胤,吴小候,罗春丽.PLCε基因通过NF-κB/p65途径抑制肾癌786-0细胞的增殖[J].陆军军医大学学报(原第三军医大学学报),2014,36(19):2010-2015. DOI：

　Yang Xue,Ou Liping,Wang Xiaorong,Wang Yin,Wu Xiaohou,Luo Chunli.Knockdown of PLCε gene inhibits proliferation in human 786-0 renal cancer cells via NF-κB/p65 pathway[J].J Amry Med Univ (J Third Mil Med Univ),2014,36(19):2010-2015. DOI：

摘要

目的探讨RNA干扰技术沉默人源磷脂酶Cε（PLCε）基因对肾癌786-0细胞p65核转位的调控，对786-0细胞增殖的影响及其机制。方法用携带PLCε shRNA基因的真核表达质粒pGenesil-PLCε转染786-0细胞，克隆形成实验检测786-0细胞的增殖，RT-PCR检测PLCε、c-Myc和COX-2 mRNA的表达，Western blot检测PLCε、c-Myc、COX-2和p65总蛋白及p65在细胞核的表达，细胞免疫荧光检测p65在细胞核与细胞质的定位。同时应用p65特异的抑制剂BAY11-7082处理786-0细胞，Western blot检测c-Myc和COX-2蛋白的表达。结果转染pGenesil-PLCε质粒的786-0细胞生长明显受抑制；转染pGenesil-PLCε质粒细胞中PLCε、c-Myc和COX-2 mRNA和蛋白表达水平均较对照组pGenesil-NP明显下调（P0.01），且抑制核中p65蛋白的表达（P0.01），细胞免疫荧光结果显示转染pGenesil-PLCε质粒组p65在细胞核中的表达较对照组pGenesil-NP明显下降；BAY11-7082抑制c-Myc和COX-2蛋白的表达（P0.01），且呈时间浓度依赖性。结论沉默PLCε基因后可能是通过抑制p65的核转位及c-Myc、COX-2的表达抑制786-0细胞的增殖。

Abstract

Objective To determine the effect of silencing phospholipase Cε (PLCε) gene by RNA interference on the nuclear translocation of p65 and cell proliferation in human 786-0 renal cancer cells. Methods A PLCε specific shRNA expression vector pGenesil-PLCε was transfected into 786-0 cells by Lipofectamine 2000. Colony cell survival assay were used to determine effects on cellular proliferation ability after transfection. The expression of PLCε

c-Myc and COX-2 was detected by RT-PCR. The expression of PLCε

c-Myc

COX-2

total p65 and p65 in the nucleus were detected by Western blotting. Immunofluorescence assay was used to detect the location of p65. The expression of c-Myc and COX-2 was also measured by Western blotting after BAY11-7082

an inhibitor of p65

treatment with different doses for different time periods. Results 786-0 cells proliferation was inhibited after PLCε shRNA treatment in vitro. In 786-0 renal cancer cells

the expression of PLCε

transcription factor p65 in the nucleus and its downstream gene c-Myc and COX-2 was decreased using PLCε specific shRNA (P0.01). Immunofluorescence assay results showed that p65 translocated to the nucleus was suppressed after PLCε shRNA transfection. The expression of c-Myc and COX-2 at protein level were inhibited by BAY11-7082 in a dose- and time-dependent manner (P0.01). Conclusion Silencing PLCε inhibits 786-0 cell proliferation via inhibiting nuclear translocation of p65 and expression of c-Myc and COX-2.

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