Antisense RNA of c-myc gene enhances sensitivity of osteosarcoma cells to cisplatin-induced apoptosis

李宏维; 叶凯山; 吴卓; 王拴科

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Antisense RNA of c-myc gene enhances sensitivity of osteosarcoma cells to cisplatin-induced apoptosis

更新时间：2026-01-09

- Antisense RNA of c-myc gene enhances sensitivity of osteosarcoma cells to cisplatin-induced apoptosis
- Journal of Army Medical University Vol. 36, Issue 24, Pages: 2463-2466(2014)
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  CLC： R73-362;R738.1;R979.19
- Published：2014
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李宏维,叶凯山,吴卓,王拴科.反义RNA抑制c-myc表达增强骨肉瘤细胞对顺铂的敏感性[J].陆军军医大学学报(原第三军医大学学报),2014,36(24):2463-2466.

　Li Hongwei,Ye Kaishan,Wu Zhuo,Wang Shuanke.Antisense RNA of c-myc gene enhances sensitivity of osteosarcoma cells to cisplatin-induced apoptosis[J].J Amry Med Univ (J Third Mil Med Univ),2014,36(24):2463-2466.
李宏维,叶凯山,吴卓,王拴科.反义RNA抑制c-myc表达增强骨肉瘤细胞对顺铂的敏感性[J].陆军军医大学学报(原第三军医大学学报),2014,36(24):2463-2466. DOI：

　Li Hongwei,Ye Kaishan,Wu Zhuo,Wang Shuanke.Antisense RNA of c-myc gene enhances sensitivity of osteosarcoma cells to cisplatin-induced apoptosis[J].J Amry Med Univ (J Third Mil Med Univ),2014,36(24):2463-2466. DOI：

摘要

目的通过对骨肉瘤细胞MG-63和U2OS c-myc基因干扰，初步探讨顺铂对c-myc低表达骨肉瘤细胞凋亡的影响。方法以腺病毒为载体，构建表达反义c-myc的重组腺病毒（Ad-Asc-myc），并在体外感染骨肉瘤细胞MG-63和U2OS，与不同浓度及不同时间点的顺铂作用后，采用Western blot方法检测干扰水平；瑞氏染色和MTT检测顺铂对骨肉瘤细胞MG-63和U2OS凋亡作用。结果构建包装的腺病毒Ad-Asc-myc体外感染MG-63和U2OS 48h后，可明显降低c-myc蛋白表达；随着顺铂浓度和处理时间增加可增强对细胞增殖的抑制。结论抑制c-myc的表达可以增强骨肉瘤对化疗的敏感性。

Abstract

Objective To investigate whether down-regulation of c-myc gene with antisense RNA can increase the sensitivity of osteosarcoma cells MG-63 and U2OS to cisplatin-induced apoptosis. Methods The recombinant adenovirus (Ad-Asc-myc) encoding antisense RNA targeting c-myc was constructed and used to transfect osteosarcoma cells MG-63 and U2OS. The expression of c-myc gene was analyzed by quantitative real-time PCR and Western blotting. The change of the sensitivity to cisplain-induced apoptosis was assayed by MTT assay. The shape of osteosarcoma cells was observed by Wright’s staining. Results Ad-Asc-myc down-regulated the expression of c-myc gene after transfection of MG-63 and U2OS cells for 48 h

which resulted in an increase of cell proliferation inhibition induced by cisplatin in a dose- and time-dependent manner. Conclusion The inhibition of c-myc gene expression increases the chemotherapy sensitivity of osteosarcoma.

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