Role and mechanism of Wnt5a regulating proliferation and epithelial mesenchymal transition in A549 spheres

胡欣春; 徐建军; 喻东亮; 彭金华; 魏益平

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Role and mechanism of Wnt5a regulating proliferation and epithelial mesenchymal transition in A549 spheres

更新时间：2026-01-09

- Role and mechanism of Wnt5a regulating proliferation and epithelial mesenchymal transition in A549 spheres
- Journal of Army Medical University Vol. 36, Issue 18, Pages: 1890-1894(2014)
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  CLC： R341;R730.23;R734.2
- Published：2014
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胡欣春,徐建军,喻东亮,彭金华,魏益平.Wnt5a 在肺癌A549细胞球增殖和上皮间质转化中的作用及机制研究[J].陆军军医大学学报(原第三军医大学学报),2014,36(18):1890-1894.

　Hu Xinchun,Xu Jianjun,Yu Dongliang,Peng Jinhua,Wei Yiping.Role and mechanism of Wnt5a regulating proliferation and epithelial mesenchymal transition in A549 spheres[J].J Amry Med Univ (J Third Mil Med Univ),2014,36(18):1890-1894.
胡欣春,徐建军,喻东亮,彭金华,魏益平.Wnt5a 在肺癌A549细胞球增殖和上皮间质转化中的作用及机制研究[J].陆军军医大学学报(原第三军医大学学报),2014,36(18):1890-1894. DOI：

　Hu Xinchun,Xu Jianjun,Yu Dongliang,Peng Jinhua,Wei Yiping.Role and mechanism of Wnt5a regulating proliferation and epithelial mesenchymal transition in A549 spheres[J].J Amry Med Univ (J Third Mil Med Univ),2014,36(18):1890-1894. DOI：

摘要

目的研究Wnt5a在A549细胞球增殖和EMT中的作用及相关机制。方法利用无血清培养出A549细胞球后应用流式细胞术对其进行CD133

CD44

表达检测。实验分为对照组、Wn5a组、p38组和Wnt5a+p38组。应用Western blot观察各组Wnt5a、p-p38、β-catenin、Vimentin和E-cadherin蛋白的表达变化，并利用MTT和裸鼠成瘤实验观察Wnt5a shRNA慢病毒感染前后A549细胞球的生长抑制率和成瘤能力。结果流式细胞术检测A549细胞球中CD133

CD44

阳性率为60.5%；Wnt5a蛋白在Wnt5a组和Wnt5a+p38组中的表达较对照组明显下调(P0.05)，p-p38蛋白、β-catenin 蛋白、Vimentin蛋白在Wnt5a组、p38组和Wnt5a+p38组的表达明显低于对照组(P0.05)，而 E-cadherin蛋白在上述3组中的表达显著高于对照组(P0.05)。Wnt5a shRNA慢病毒感染A549细胞球12、24 h和48 h时抑制率显著高于对照组(P0.05)，并且该细胞的成瘤能力较对照组显著下调。结论下调Wnt5a表达可抑制A549细胞球的增殖及EMT能力，可望成为治疗非小细胞肺癌的靶点。

Abstract

Objective To deternine the role and mechanism of Wnt5a regulating the proliferation and epithelial mesenchymal transition (EMT) in lung cancer A549 spheres. Methods A549 spheres were cultured in serum-free medium for 2 weeks. CD133

CD44

rate of those cells was detected by FACS. Four experimental groups including a control group

a Wnt5a group

a p38 group and a Wnt5a+ p38 group were designed. The protein expr

ession of Wnt5a

p-p38

β-catenin

vimentin and E-cadherin among the 4 groups was detected by Western blotting. The cell inhibitory rate and tumorigenicity were detected after Wnt5a shRNA lentivirus transfection of A549 spheres. Results CD133

CD44

rate was 60.5% in A549 spheres and 1.65% in A549 cells. Wnt5a protein expression was significantly lower in the Wnt5a and Wnt5a+ p38 groups than in the control group (P0.05). Compared to the control group

the protein expression of p-p38

β-catenin and vimentin was obviously lower but that of E-cadherin was higher in the Wnt5a

p38 and Wnt5a+ p38 groups (P0.05). The cell inhibitory rate was significantly higher and tumor size in node mice was remarkably smaller at 12

24 and 48 h after Wnt5a shRNA lentivirus transfection (P0.05). Conclusion Wnt5a expression down-regulation inhibits the proliferation and EMT of A549 spheres

and Wnt5a might be a novel target for treatment of non-small-cell lung cancer.

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