Sulfiredoxin-1 protects astrocytes against injury induced by oxygen-glucose deprivation/reoxygenation

周云川; 周杨; 巫静娴; 喻姗姗; 赵涌

您当前的位置：

首页 >

文章列表页 >

Sulfiredoxin-1 protects astrocytes against injury induced by oxygen-glucose deprivation/reoxygenation

更新时间：2026-01-09

- Sulfiredoxin-1 protects astrocytes against injury induced by oxygen-glucose deprivation/reoxygenation
- Journal of Army Medical University Vol. 36, Issue 21, Pages: 2173-2177(2014)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：
  CLC： R322.8;R363.21;R394-33
- Published：2014
- 稿件说明：
移动端阅览
周云川,周杨,巫静娴,喻姗姗,赵涌.Sulfiredoxin-1对星形胶质细胞氧糖剥夺/复氧损伤的保护作用[J].陆军军医大学学报(原第三军医大学学报),2014,36(21):2173-2177.

　Zhou Yunchuan,Zhou Yang,Wu Jingxian,Yu Shanshan,Zhao Yong.Sulfiredoxin-1 protects astrocytes against injury induced by oxygen-glucose deprivation/reoxygenation[J].J Amry Med Univ (J Third Mil Med Univ),2014,36(21):2173-2177.
周云川,周杨,巫静娴,喻姗姗,赵涌.Sulfiredoxin-1对星形胶质细胞氧糖剥夺/复氧损伤的保护作用[J].陆军军医大学学报(原第三军医大学学报),2014,36(21):2173-2177. DOI：

　Zhou Yunchuan,Zhou Yang,Wu Jingxian,Yu Shanshan,Zhao Yong.Sulfiredoxin-1 protects astrocytes against injury induced by oxygen-glucose deprivation/reoxygenation[J].J Amry Med Univ (J Third Mil Med Univ),2014,36(21):2173-2177. DOI：

摘要

目的探讨Sulfiredoxin-1（Srxn1）对氧糖剥夺/复氧（oxygen-glucose deprivation/ reoxygenation，OGD/R）诱导的星形胶质细胞损伤的保护作用。方法 ①应用RNAi技术将Srxn1干扰小发夹RNA（shRNA）及阴性载体（negative control）转染至星形胶质细胞，Real-time quantitative PCR（QPCR）和Western blot检测转染后Srxn1的基因和蛋白表达水平；②实验分为control组、control+OGD/R组、negative control（NC）+OGD/R组、sh-Srxn1+OGD/R组（n=3），细胞转染72 h后建立OGD模型，6 h后予以复氧，模拟再灌注模型；③复氧12 h后，MTS法检测细胞活性，Hoechst 33342检测细胞凋亡率，Western blot检测细胞Bcl-2和Bax的蛋白表达水平。结果 ①shRNA-Srxn1转染细胞后，与空白组和阴性组比较，干扰组Srxn1 mRNA及蛋白表达水平明显降低，分别降低47%和58.5%；②MTS结果显示，干扰sh-Srxn1+OGD/R组细胞活性较control+OGD/R组显著下降[（38.79±2.14） vs （69.35±10.54）

P0.05]；Hoechst 33342结果显示，干扰sh-Srxn1+OGD/R组细胞凋亡较control+OGD/R组明显增加[（51.67±7.64） vs （25.00±5.00）

P0.01]；③Western blot结果显示，与control+OGD/R组比较，干扰sh-Srxn1+OGD/R组细胞Bcl-2的表达明显降低（P0.05），Bax的表达明显上调（P0.05），Bcl-2/Bax比值显著降低（P0.01）。结论 Srxn1对OGD/R引起的星形胶质细胞的氧化损伤具有重要的保护作用，这种保护可能与其抗凋亡作用相关。

Abstract

Objective To determine the protective effect of sulfiredoxin-1 (Srxn1) on the injured astrocytes induced by oxygen-glucose deprivation/reoxygenation (OGD/R). Methods Lentivirus vector encoding Srxn1-shRNAs and a negative control were used to transfect to primary rat cortical astrocytes. Real-time quantitative PCR (q-PCR) and Western blotting were used to detect the expression of Srxn1 at mRNA and protein levels. The OGD/R model of astrocytes was established by OGD for 6 h followed by reoxygenation for 12 h to construct an in vitro model of ischemia reperfusion (for transfected cells

the establishment was started in 72 h after transfection). Then the normal control astrocytes were cultured under or not OGD/R

so were the astrocytes transfected by sh-Srxn1 or negative shRNAs. After reoxygenation for 12 h

the expression of Srxn1 was measured by q-PCR and Western blotting. Cell viability was determined by MTS assay. Apoptosis was measured by Hoechst 33342 staining. And the levels of Bcl-2 and Bax were measured by Western blotting. Results After transfection of sh-Srxn1

the mRNA and protein expression levels of Srxn1 were significantly decreased by 47.0% and 58.5% than the cells without transfection and negative control group. MTS assay indicated that interference of Srxn1 resulted in a decrease of cell viability compared with control+OGD/R group (38.79±2.14 vs 69.35±10.54

P0.05). Hoechst 33342 staning displayed that the apoptotic rate was significantly higher in sh-Srxn1+OGD/R group than in control+OGD/R group (51.67±7.64 vs 25.00±5.00

P0.01). And Western blotting showed that Srxn1 interference resulted in a decrease in Bcl-2 expression (P0.05)

an increase in Bax expression (P0.05)

and a significant decrease in the ratio of Bcl-2/Bax (P0.01)

when compared with control+OGD/R group. Conclusion Srxn1 exerts a protective effect on the oxidative stress injury in the astrocytes induced by OGD/R

which might be due to its anti-apoptosis effect.

关键词

Keywords

references

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

BAMBI inhibits apoptosis in glutamate induced neurons and astrocytes in spinal dorsal horn of rats via regulating autophagy

Sulfiredoxin-1 protects rat astrocytes from ischemia-induced damage

In vitro relationship of apolipoprotein E polymorphisms and excitatory amino acids in astrocyte injury

Effects of hypoxic preconditioning on hypoxia/reoxygenation tolerance of astrocytes in vitro

Expression of miR-616 in osteosarcoma and its role in proliferation, apoptosis, migration and invasion of tumor cells

Related Author

张志鑫

崔应麟

李彦杰

任锟

邢若星

喻姗姗

刘远玲

陈曦

Related Institution

Department of Pathology， Second Affiliated Hospital， Army Medical University （Third Military Medical University）

Department of Immunology, Faculty of Pharmacy and Laboratory Medicine， Army Medical University （Third Military Medical University）

Department of Laboratory Animal Science， College of Basic Medical Sciences, Faculty of Pharmacy and Laboratory Medicine， Army Medical University （Third Military Medical University）

Department of Pharmaceutical Analysis, Faculty of Pharmacy and Laboratory Medicine， Army Medical University （Third Military Medical University）

Department of Thoracic Surgery, Institute of Surgery Research, Daping Hospital, Third Military Medical University

⁰