Alteration of CRF mRNA expression in hypothalamus of SERT-knockout mice exposed to elevated plus maze

文玲; 邓永兵; 朱小云; 蒋雪

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Alteration of CRF mRNA expression in hypothalamus of SERT-knockout mice exposed to elevated plus maze

更新时间：2026-01-09

- Alteration of CRF mRNA expression in hypothalamus of SERT-knockout mice exposed to elevated plus maze
- Journal of Army Medical University Vol. 36, Issue 18, Pages: 1876-1880(2014)
- 作者机构：
- 作者简介：
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- DOI：
  CLC： R338.27;R363.27;R394.3
- Published：2014
- 稿件说明：
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文玲,邓永兵,朱小云,蒋雪.5-羟色胺转运体敲除小鼠在高架十字迷宫中下丘脑CRF mRNA表达的变化[J].陆军军医大学学报(原第三军医大学学报),2014,36(18):1876-1880.

　Wen Ling,Deng Yongbing,Zhu Xiaoyun,Jiang Xue.Alteration of CRF mRNA expression in hypothalamus of SERT-knockout mice exposed to elevated plus maze[J].J Amry Med Univ (J Third Mil Med Univ),2014,36(18):1876-1880.
文玲,邓永兵,朱小云,蒋雪.5-羟色胺转运体敲除小鼠在高架十字迷宫中下丘脑CRF mRNA表达的变化[J].陆军军医大学学报(原第三军医大学学报),2014,36(18):1876-1880. DOI：

　Wen Ling,Deng Yongbing,Zhu Xiaoyun,Jiang Xue.Alteration of CRF mRNA expression in hypothalamus of SERT-knockout mice exposed to elevated plus maze[J].J Amry Med Univ (J Third Mil Med Univ),2014,36(18):1876-1880. DOI：

摘要

目的研究5-羟色胺转运体(serotonin transporter，SERT)敲除小鼠在高架十字迷宫（elevated plus maze

EPM）中下丘脑促肾上腺皮质激素释放因子(corticotrophin-releasing factor

CRF) mRNA表达的变化。方法将小鼠按基因型（SERT

+/+

、SERT

+/-

、SERT

-/-

）分为对照组和EPM组，每组10只，EPM组利用高架十字迷宫作为应激原刺激小鼠。测定各组小鼠下丘脑以及室旁核部位CRF mRNA的表达。结果在基础条件下（对照组），SERT敲除小鼠下丘脑及室旁核的CRF mRNA表达降低（P0.05）；EPM刺激下（EPM组），SERT

+/-

和SERT

-/-

小鼠在下丘脑及室旁核的CRF mRNA表达显著高于SERT

+/+

(P0.01)；构建的CRF mRNA探针反义链在下丘脑PVN部位检测到高密度的CRF mRNA，而正义链却不能探测到CRF mRNA；EPM组hnRNA SERT-/-小鼠表达较对照组明显增高(P0.01)。结论应激能明显促进下丘脑CRF mRNA的表达。SERT敲除小鼠的异常应激与CRF mRNA的表达升高有关。

Abstract

Objective To determine the expression alteration of corticotrophin-releasing factor (CRF) mRNA in the hypothalamus of serotonin transporter (SERT)-knockout mice exposed to elevated plus maze (EPM). Methods Mice were divided into 2 groups

a control group and an EPM group

according their genotypes (SERT

+/+

SERT

+/-

and SERT

-/-

)

with 10 mice in each group. EPM was used as a stressor to stimulate the

mice. Then anxious like behavior was observed

and the CRF mRNA expression in the hypothalamus and nucleus paraventricularis (PVN) were measured. Results The non-stress expression of CRF mRNA in the hypothalamus and PVN were lower in both SERT

+/-

and SERT

-/-

mice than in SERT

+/+

mice (P0.05). Exposure to the EPM significantly increased CFR mRNA in the hypothalamus and PVN of SERT

+/-

and SERT

-/-

mice (P0.05)

higher than that of the SERT

+/+

mice. CRF mRNA antisense probes in the PVN detected high density of CRF mRNA

but CRF sense mRNA did not. With exposure to the EPM

the expression of hnRNA in SERT

-/ -

mice was significantly higher than that in the control group (P0.01). Conclusion Stress from exposure to EPM significantly increases CRF mRNA expression in the PVN and hypothalamus of SERT

+/-

and SERT

-/-

mice. The abnormal stress reaction of SERT-knockout mice is associated with the increased CRF mRNA expression.

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