Objective        To construct a lentiviral vector carrying human fevritin heavy chaik 1 (FTH1) gene and observe its expression in neuroblastoma SK-N-SH cells.       Methods        The FTH1 gene was transferred into SK-N-SH cells by using a lentiviral vector. The cells in experimental group (SK-N-SH-FTH1)

mock group (SK-N-SH-GFP) and blank group (SK-N-SH) were cultured in

the medium containing 500 μmol/L ferric ammonium citrate (FAC) for 4 passages

respectively. The effect of FTH1 over-expression or FAC on cell viability was tested by CCK-8 assay. Prussian blue staining and transmission electron microscopy were used to determine iron accumulation in the cells. MR imaging was performed to determine the changes of T

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WI signal intensity of cells in vitro. The expression of FTH1 gene was detected by Western blot analysis.       Results       CCK-8 assay showed there was no significant difference in cell viability among the 3 groups of cells cultured in the medium without FAC (SK-N-SH-FTH1: 0.99±0.03

SK-N-SH-GFP: 0.99±0.02

SK-N-SH: 0.98±0.05

P0.05). However

when the culture medium contained 500 μmol/L FAC for 48 h

the cell viability was significantly reduced (SK-N-SH-FTH1:0.73±0.08; SK-N-SH-GFP: 0.76±0.02; SK-N-SH: 0.73±0.39

P0.05). Prussian blue staining and transmission electron microscopy showed a great large amount of iron particles were accumulated in the experimental cells. Accordingly

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WI signal intensity was significantly decreased in the experimental cells than the other 2 types of control cells. Western blot analysis confirmed that FTH1 was expressed continuously and stably in the experimental cells even at the 4th passage.       Conclusion        The recombinant lentivirus vector carrying FTH1 gene is constructed successfully

and its stable over-expression results in iron accumulation in SK-N-SH cells after infection.