Correlation of single nucleotide polymorphism of let-7a gene regulatory region with diabetic nephropathy in 108 cases of Chinese Han population

周吉; 彭睿; 罗勇军; 李天驹; 孙艳; 尹频; 彭惠民; 张政

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Correlation of single nucleotide polymorphism of let-7a gene regulatory region with diabetic nephropathy in 108 cases of Chinese Han population

更新时间：2026-01-09

- Correlation of single nucleotide polymorphism of let-7a gene regulatory region with diabetic nephropathy in 108 cases of Chinese Han population
- Journal of Army Medical University Vol. 36, Issue 12, Pages: 1312-1316(2014)
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  CLC： R363.25;R587.2;R690.02
- Published：2014
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周吉,彭睿,罗勇军,李天驹,孙艳,尹频,彭惠民,张政.108例汉族人群let-7a基因调控区多态性与糖尿病肾病的相关性分析[J].陆军军医大学学报(原第三军医大学学报),2014,36(12):1312-1316.

　Zhou Ji,Peng Rui,Luo Yongjun,Li Tianju,Sun Yan,Yin Pin,Peng Huimin,Zhang Zheng.Correlation of single nucleotide polymorphism of let-7a gene regulatory region with diabetic nephropathy in 108 cases of Chinese Han population[J].J Amry Med Univ (J Third Mil Med Univ),2014,36(12):1312-1316.
周吉,彭睿,罗勇军,李天驹,孙艳,尹频,彭惠民,张政.108例汉族人群let-7a基因调控区多态性与糖尿病肾病的相关性分析[J].陆军军医大学学报(原第三军医大学学报),2014,36(12):1312-1316. DOI：

　Zhou Ji,Peng Rui,Luo Yongjun,Li Tianju,Sun Yan,Yin Pin,Peng Huimin,Zhang Zheng.Correlation of single nucleotide polymorphism of let-7a gene regulatory region with diabetic nephropathy in 108 cases of Chinese Han population[J].J Amry Med Univ (J Third Mil Med Univ),2014,36(12):1312-1316. DOI：

摘要

目的探寻循环miRNA let-7a与糖尿病肾病（diabetic nephropathy，DN）的关系，分析并评估let-7a调控区域单核甘酸多态性（single nucleotide polymorphism

SNP）对DN疾病的易感性。方法 Real-time PCR检测病例组及对照组中let-7a的表达水平。生物信息学方法预测并筛选3个位于let-7a前体（let-7a-2）启动子调控区域标签SNP(Tag SNP)，采用聚合酶链式反应-限制性片段长度多态性（RFLP-PCR）法对108例糖尿病肾病（DN）、104例糖尿病（DM）患者及62例健康人对照（CON）3个SNP进行基因分型。结果 Real-time PCR结果显示let-7a在DN、DM、CON三组中表达水平有显著差异（

0.05）。let-7a-2 基因调控区域rs1143770多态位点的CT杂合子基因型在DN、DM、CON三组间有显著差异（

0.05），且在DM组和DN组CT+TT两基因型均依次较CON组显著升高（

=0.049；

=0.001)。结论 miRNA let-7a可能参与调控DN疾病，其启动子调控区rs1143770多态与中国汉族人群糖尿病肾病相关，可能是个潜在的功能位点。

Abstract

Objective To explore the relationship between the circulating miRNA let-7a and diabetic nephropathy (DN)

and to evaluate the susceptibility of the single nucleotide polymorphism (SNP) of let-7a regulatory region to DN. Methods Real-time PCR was carried out to detect the expression level of let-7a in the patients and control groups. We used bioinformatics methods to predict and filter out 3 Tag SNPs in let-7a gene

promoter regulatory region. Polymerase chain reaction-restriction fragment length polymorphism (RFLP-PCR) was executed to genotype 108 DN patients (DN group)

104 patients with diabetes (DM group) and 62 cases of healthy controls (CON group). Results Real-time PCR results showed that the expression level of let-7a were significantly different among the DN

CON groups (

0.05). A variant rs1143770 was found in let-7a-2 regulatory region and the distributions of CT genotypes were significantly different among the 3 groups (

0.05). The CT+TT genotype frequencies were significantly higher in the DM and DN groups than in the CON group (

=0.049

=0.001). Conclusion miRNA let-7a may participate in the regulation of DN occurrence

and rs1143770 in let-7a-2 regulatory region is significantly associated with the increased risk of DN in Chinese Han population and might be regarded as a potential functionol site.

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