Injection of CART via lateral ventricle enhances insulin secretion in rats with type 2 diabetes

李聃丹; 侯君; 应懿; 樊莉; 谯魏; 周世文

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Injection of CART via lateral ventricle enhances insulin secretion in rats with type 2 diabetes

更新时间：2026-01-09

- Injection of CART via lateral ventricle enhances insulin secretion in rats with type 2 diabetes
- Journal of Army Medical University Vol. 36, Issue 8, Pages: 793-796(2014)
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  CLC： R322.57;R587.1;R977.6
- Published：2014
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李聃丹,侯君,应懿,樊莉,谯魏,周世文.侧脑室注射CART促进2型糖尿病大鼠胰岛素分泌[J].陆军军医大学学报(原第三军医大学学报),2014,36(08):793-796.

　Li Dandan,Hou Jun,Ying Yi,Fan Li,Qiao Wei,Zhou Shiwen.Injection of CART via lateral ventricle enhances insulin secretion in rats with type 2 diabetes[J].J Amry Med Univ (J Third Mil Med Univ),2014,36(08):793-796.
李聃丹,侯君,应懿,樊莉,谯魏,周世文.侧脑室注射CART促进2型糖尿病大鼠胰岛素分泌[J].陆军军医大学学报(原第三军医大学学报),2014,36(08):793-796. DOI：

　Li Dandan,Hou Jun,Ying Yi,Fan Li,Qiao Wei,Zhou Shiwen.Injection of CART via lateral ventricle enhances insulin secretion in rats with type 2 diabetes[J].J Amry Med Univ (J Third Mil Med Univ),2014,36(08):793-796. DOI：

摘要

目的测定并比较侧脑室注射可卡因-苯丙胺转录调节肽（cocaine and amphetamine regulated transcript

CART）24 h后2型糖尿病大鼠与正常组大鼠空腹血糖（FBG）、血清总胆固醇（TC）、甘油三酯（TG）、高密度脂蛋白（HDLC）、低密度脂蛋白（LDLC）、糖化血红蛋白（HbA

C）、血清和组织胰岛素含量以及胰腺组织胰岛素（insulin，INS) mRNA相对表达量，明确在2型糖尿病大鼠侧脑室注射CART后胰腺中胰岛素含量变化。方法采用高糖高脂饮食/大剂量链脲佐菌素（STZ）制备2型糖尿病大鼠模型，侧脑室注射人工脑脊液（artificial cerebrospinal fluid，ACSF）/CART 24 h后，测定血液中FBG、TC、TG、HDLC、LDLC、HbA

C，血清和胰腺组织中胰岛素含量；采用免疫荧光法对大鼠胰腺CART、INS进行定位分析；采用荧光定量PCR测定胰腺组织中INS mRNA相对表达量。结果对2型糖尿病大鼠模型进行侧脑室注射CART后，FBG明显下降（

0.05），但未达到正常水平，胰腺中INS mRNA相对表达量上调，与糖尿病大鼠侧脑室注射ACSF相比较具有统计学差异（

0.05）。结论糖尿病大鼠侧脑室注射CART后能增加胰腺中CART的表达量及胰岛素含量，表明CART有促进胰岛β细胞分泌胰岛素的作用。

Abstract

Objective To detect the biochemical parameters

insulin content in serum and tissue and mRNA level of insulin upon injection of cocaine and amphetamine regulated transcript (CART) via lateral ventricle for 24 h in normal rats and type 2 diabetes rats

and to explicit the role of CART on insulin secretion in pancreas. Methods Type 2 diabetes model was established by food with high fat and sugar/STZ. After i

njection of artificial cerebrospinal fluid (ACSF) or CART via lateral ventricle for 24 h

biochemical parameters and insulin content of both tissue and serum were detected. CART and insulin positioning analysis was accomplished by double immunofluorescent labeling. Insulin mRNA level in pancreas was measured by quantitative RT-PCR. Results Compared with ACSF injection

fasting blood glucose (FBG) decreased in type 2 diabetes rats after CART injection (

0.05)

although it did not return to the normal level. Furthermore

the mRNA level of insulin in pancreas increased significantly after injection of CART in type 2 diabetes rats (

0.05). Conclusion Both CART and insulin contents increase obviously in pancreas after CART injection in lateral ventricle of type 2 diabetes rats. CART may play important roles in enhancing insulin secretion in pancreas.

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