Objective        To establish a mouse model of airway inflammation induced by lymphocyte-derived microparticels (LMPs) and investigate the role of LMPs in the incidence of airway inflammation.       Methods      After human T-leukemia cell line 6T-CEM was treated with actinomycin D for 24 h

the LMPs were collected. A total of 12 BALB/c mice were divided into normal group and LMPs group (n=6)

and their airways were infused with 100 μL normal saline and 50 μg/mL LMPs respectively. In 24 h later

all mice were killed

and then the lung tissues were accessed. HE staining was used to analyz

e the morphological changes of lung tissues. ELISA

Western blotting and quantity RT-PCR assays were used to measure the expression of immune-related molecules

NLRP3

TLR4

IL-18 and IL-1β at protein and mRNA levels.       Results        HE staining revealed alveolar cells aggregation and lymphocytic infiltration in the lung tissues of LMPs-treated mice. ELISA showed that the secreted protein levels of IL-18 and IL-1β were significantly higher in the lung tissues in LMPs-treated mice than normal control (

P

0.01). Quantity RT-PCR and Western blot assays showed that the mRNA and protein expression of NLRP3

TLR4

IL-18 and IL-1β were significantly increased in the lung tissues in LMPs-treated mice than normal control (

P

0.01).       Conclusion        LMPs induce airway inflammation in mice

and the inflammatory response is related to NLRP3

TLR4

IL-18 and IL-1β.