Effects of bone homeostasis induced by high-dose 1,25(OH)<sub>2</sub>D<sub>3</sub> in pubertal mice

黄启钊; 戚华兵; 王晓凤; 朱莹; 王权; 王先行; 杜晓兰; 陈林

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Effects of bone homeostasis induced by high-dose 1,25(OH)₂D₃ in pubertal mice

更新时间：2026-01-09

- Effects of bone homeostasis induced by high-dose 1,25(OH)₂D₃ in pubertal mice
- Journal of Army Medical University Vol. 36, Issue 8, Pages: 774-779(2014)
- 作者机构：
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- DOI：
  CLC： R322.71;R339.31;R977.24
- Published：2014
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黄启钊,戚华兵,王晓凤,朱莹,王权,王先行,杜晓兰,陈林.大剂量1,25二羟维生素D3对发育期小鼠骨稳态的影响[J].陆军军医大学学报(原第三军医大学学报),2014,36(08):774-779.

　Huang Qizhao,Qi Huabing,Wang Xiaofeng,Zhu Ying,Wang Quan,Wang Xianxing,Du Xiaolan,Chen Lin.Effects of bone homeostasis induced by high-dose 1,25(OH)2D3 in pubertal mice[J].J Amry Med Univ (J Third Mil Med Univ),2014,36(08):774-779.
黄启钊,戚华兵,王晓凤,朱莹,王权,王先行,杜晓兰,陈林.大剂量1,25二羟维生素D3对发育期小鼠骨稳态的影响[J].陆军军医大学学报(原第三军医大学学报),2014,36(08):774-779. DOI：

　Huang Qizhao,Qi Huabing,Wang Xiaofeng,Zhu Ying,Wang Quan,Wang Xianxing,Du Xiaolan,Chen Lin.Effects of bone homeostasis induced by high-dose 1,25(OH)2D3 in pubertal mice[J].J Amry Med Univ (J Third Mil Med Univ),2014,36(08):774-779. DOI：

摘要

目的观察大剂量1

25二羟维生素D3[1

25(OH)

]

对小鼠发育期骨稳态的影响。方法怀孕13.5 d的C3h小鼠分成3组，每日分别经腹腔注射1%的乙醇溶液（对照组），0.5 ng/g的1

25(OH)

（低剂量组）和5 ng/g的1

25(OH)

（大剂量组），持续至孕鼠分娩。新生小鼠从第3天开始隔天注射相同剂量的1

25(OH)

，连续1个月。观察小鼠出生以及身长、体质量等生长变化情况；1月龄的小鼠分别行X线检查、Micro-CT扫描，胫骨组织石蜡包埋、切片后经HE、藏红固绿染色，行组织学、骨形态计量分析，观察不同分组发育期小鼠骨骼结构及骨代谢的变化情况。结果从E13.5 d开始注射不同剂量的1

25(OH)

对孕鼠怀孕过程和小鼠出生没有明显的影响，出生后第7天开始，大剂量组小鼠身长、体质量明显低于对照组（

0.05），1月龄时，差异增大，大剂量组身长、体质量显著低于对照组和小剂量组（

0.01）；X线检测显示1月龄大剂量组小鼠发生病理性骨折（n=10），其余2组未发现；Micro-CT结果提示大剂量组小鼠骨量较对照组显著降低（

0.01），低剂量组小鼠仅骨皮质厚度有所增加(

0.05)；胫骨组织切片形态计量分析显示大剂量组小鼠破骨细胞数量较其余2组显著增加（

0.01），而低剂量组破骨细胞数量的增加差异无统计学意义；低剂量组成骨细胞数量较其余2组有明显增加（

0.05）。结论大剂量1

25(OH)

显著降低C3h小鼠骨量，同时使骨皮质菲薄，骨骼质量变差，从而易骨折。这种作用主要是通过促进破骨细胞的形成实现的。

Abstract

Objective To investigate the effects of high-dose 1

25-dihydroxy-vitamin D

25(OH)

]

on the bone homeostasis of mice in puberty. Metho

ds Pregnant C3h mice were allocated in 3 groups with intraperitoneal (i.p.) injection of 1% ethanol (control group)

0.5 ng/g 1

25(OH)2D3 (low-dose group) and 5 ng/g 1

25(OH)

(high-dose group)

respectively

from E13.5 until delivery. The newborns of each group were injected with the same dose of 1

25(OH)

as their mothers did from day 3 after birth

every 2 days

to 1 month. The body weight and length of the newborns were recorded per week. All mice were subjected to X-ray filming and Micro-CT scanning at 1 month and then sacrificed. The tibiae were dissected and decalcified for histological and histomorphometry analysis. Results The injection of 1

25(OH)

from E13.5 had no significant effect on pregnancy and birth. Compared with the control group

the mice in the high-dose group had significantly shortened body length and lightened body weight from day 7 after birth (

0.05). At 1 month after birth

the significant differences were enlarged (

0.001). X-ray filming showed spontaneous bone fracture of mice with high-dose 1

25(OH)

injection

which did not exist in other groups. Micro-CT scanning demonstrated a significant decrease in bone volume of mice in the high-dose group (

0.001)

while in the low-dose group only the thickness of cortical bone had increased (

0.05). Histological analysis revealed significantly increased number of osteoclasts in the high-dose group compared with other groups (

0.001)

while only a slight increase which had no statistic significance in the number of osteoclasts from the low-dose group was detected. Only mice from the low-dose group had significantly increased osteoblasts (

0.05). Conclusion High dose of 1

25(OH)

results in decreased bone volume and compromised bone integrity

leading to higher risk of bone fracture. These negative effects may predominantly result from an increase in the p

opulation of osteoclasts.

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Effects of bone homeostasis induced by high-dose 1,25(OH)2D3 in pubertal mice

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摘要

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Effects of bone homeostasis induced by high-dose 1,25(OH)₂D₃ in pubertal mice