Effects of miR-144 overexpression lentivirus on pathological processes of synovial tissue in arthritis mouse model

闫演飞; 康菲; 杨波; 苗莹; 董世武

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Effects of miR-144 overexpression lentivirus on pathological processes of synovial tissue in arthritis mouse model

更新时间：2026-01-09

- Effects of miR-144 overexpression lentivirus on pathological processes of synovial tissue in arthritis mouse model
- Journal of Army Medical University Vol. 36, Issue 10, Pages: 1031-1035(2014)
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  CLC： R-322;R363.25;R684.3
- Published：2014
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闫演飞,康菲,杨波,苗莹,董世武.miR-144过表达慢病毒对关节炎模型小鼠滑膜组织病理进程的影响[J].陆军军医大学学报(原第三军医大学学报),2014,36(10):1031-1035.

　Yan Yanfei,Kang Fei,Yang Bo,Miao Ying,Dong Shiwu.Effects of miR-144 overexpression lentivirus on pathological processes of synovial tissue in arthritis mouse model[J].J Amry Med Univ (J Third Mil Med Univ),2014,36(10):1031-1035.
闫演飞,康菲,杨波,苗莹,董世武.miR-144过表达慢病毒对关节炎模型小鼠滑膜组织病理进程的影响[J].陆军军医大学学报(原第三军医大学学报),2014,36(10):1031-1035. DOI：

　Yan Yanfei,Kang Fei,Yang Bo,Miao Ying,Dong Shiwu.Effects of miR-144 overexpression lentivirus on pathological processes of synovial tissue in arthritis mouse model[J].J Amry Med Univ (J Third Mil Med Univ),2014,36(10):1031-1035. DOI：

摘要

目的探讨miR-144过表达慢病毒对于关节炎模型小鼠滑膜组织病理进程的作用及其可能的机制。方法将Ⅱ型胶原乳剂注射至BALB/c小鼠尾根部皮下构建由Ⅱ型胶原诱导的关节炎(collagen-induced arthritis，CIA)小鼠模型，再以miRNA-144高表达慢病毒进行尾静脉注射，然后对模型小鼠滑膜组织进行免疫组化检测Cadherin-11和TNF-α的表达和分布，并用HE染色检测滑膜组织的病理变化特点。结果模型小鼠滑膜组织表现出与类风湿性关节炎病理学进程相似的特点，miRNA-144高表达慢病毒处理较生理盐水和空载慢病毒处理组模型小鼠的滑膜组织的炎症反应明显减轻，且TNF-α表达较生理盐水和空载体慢病毒处理组显著下调（

0.05），进一步检测Cadherin-11的表达亦显著降低（

0.05）。结论机体中miRNA-144增加可减弱或抑制关节炎模型小鼠中滑膜组织的炎症反应，并下调其TNF-α的表达，且这一作用可能是miRNA-144抑制滑膜细胞中Cadherin-11的效应。

Abstract

Objective To determine the role of miR-144-overexpressed lentivirus in the pathological process of synovial tissue in arthritis mouse model and its possible mechanism. Methods Type Ⅱ collagen-induced arthritis (CIA) mouse models were obtained by injecting the emulsion of type II collagen into subcutaneous tail base of BALB/c mice. Then miRNA-144 overexpression lentivirus was injected into the tail vein. The expression and distribution of cadherin-11 and TNF-α in the synovial tissue of model mice were detected through immunofluorescence staining a

nd the pathological characteristics were studied with HE staining. Results The mouse model exhibited similar characteristics as the pathological processes of synovial tissue in rheumatoid arthritis. Compared with saline and dummy vector lentivirus-treated groups

the inflammation of the synovial tissue was significantly reduced in the miRNA-144 overexpression lentivirus-treated group

whose TNF-α expression and cadherin-11 protein expression were also significantly lower (P0.05

0.05). Conclusion The increase of miRNA-144 content reduces the inflammation of synovial tissue in the CIA mouse models

and down-regulates the expression of TNF-α which may result from miRNA-144-induced cadherin-11inhibition.

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