Allopurinol improves cardiac function of chronic heart failure rats

王真真; 刘地川; 蔡敏; 吴健

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Allopurinol improves cardiac function of chronic heart failure rats

更新时间：2026-01-09

- Allopurinol improves cardiac function of chronic heart failure rats
- Journal of Army Medical University Vol. 36, Issue 10, Pages: 1021-1025(2014)
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  CLC： R541.61;R965;R977.3
- Published：2014
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王真真,刘地川,蔡敏,吴健.别嘌呤醇对慢性心衰大鼠心功能的改善作用[J].陆军军医大学学报(原第三军医大学学报),2014,36(10):1021-1025.

　Wang Zhenzhen,Liu Dichuan,Cai Min,Wu Jian.Allopurinol improves cardiac function of chronic heart failure rats[J].J Amry Med Univ (J Third Mil Med Univ),2014,36(10):1021-1025.
王真真,刘地川,蔡敏,吴健.别嘌呤醇对慢性心衰大鼠心功能的改善作用[J].陆军军医大学学报(原第三军医大学学报),2014,36(10):1021-1025. DOI：

　Wang Zhenzhen,Liu Dichuan,Cai Min,Wu Jian.Allopurinol improves cardiac function of chronic heart failure rats[J].J Amry Med Univ (J Third Mil Med Univ),2014,36(10):1021-1025. DOI：

摘要

目的探讨别嘌呤醇(allopurinol，ALLO)对慢性心衰大鼠心功能的保护作用及其抗氧化作用机制在其中的作用。 方法 6 周龄的雄性SD大鼠35 只，体质量（200±20）g，将35 只大鼠随机分为ALLO处理组（AMI+ALLO，n=12）、对照组（AMI+NS，n=13）和假手术组（sham-operated +NS，n=10），ALLO处理组和对照组大鼠结扎冠状动脉前降支建立急性心肌梗死（acute myocardium infarction，AMI）模型，假手术组大鼠只穿线不结扎。术后第2天，ALLO处理组给予ALLO [50 mg/(kg·d)

]

灌胃，对照组和假手术组大鼠给予生理盐水（normal saline，NS）灌胃。8周后，检测并比较分析各组大鼠的心功能、血清脑钠尿肽（brain natriuretic peptide，BNP）含量、黄嘌呤氧化酶（xanthine Oxidase，XO）蛋白表达、丙二醛（malondialdehyde，MDA）含量、线粒体呼吸链酶Ⅰ~Ⅳ的活性、心肌组织形态以及心肌诱导型氧化亚氮合酶（induce-nitri-coxide synthase，iNOS）的表达量。结果对照组与假手术组大鼠相比，大鼠心功能、线粒体呼吸链酶Ⅰ、Ⅲ、Ⅳ活性明显降低（

0.05），血清BNP含量、XO蛋白表达、MDA水平明显升高（

0.05），HE染色显示心肌组织明显水肿（

0.05），iNOS 表达升高（

0.05），证明CHF建模成功；ALLO处理组（EF：87.10%）与对照组（EF：68.18%）相比，ALLO处理组大鼠的心功能明显改善（

0.05），BNP含量、XO蛋白表达、MDA水平均下降（

0.05），大鼠线粒体呼吸链酶Ⅰ、Ⅲ、Ⅳ活性增加（

0.05），HE染色显示心肌组织水肿减轻（

0.05），iNOS表达受抑制（

0.05）。结论 CHF建模成功；ALLO可以改善CHF大鼠的心功能，其机制可能与其抗氧化作用有关。

Abstract

Objective To determine the protective effect of allopurinol (ALLO) on cardiac function in rats with chronic heart failure and its antioxidant mechanism in improving cardiac function.

Methods Thirty-five of 6-weeks-old male SD rats average weighted (200±20)g

randomly divided into the ALLO treatment group (n=12)

control group (n=13) and sham-operated group (n=10).SD rats in ALLO treatment group and the control group were established of acute myocardial infarction (AMI) models by ligation of the left anterior descending coronary artery

and SD rats of sham-operated group underwent the same procedure

including anesthesia and open-chest surgery

but the artery was not ligated. Two days after the surgery

ALLO treatment group underwent gavage administration by ALLO(50 mg/kg·d)

while the gavage administration in control group and sham-operated group were only by the saline. Eight weeks later

the cardiac function of SD rats

levels of brain natriuretic peptide (BNP)

expression of xanthine oxidase(XO)

malondialdehyde (MDA)

the activity of mitochondrial complexⅠ-Ⅳ

morphological changes in myocardial tissue and the expression of iNOS were detected and analyzed. Results Compared with the rats in sham-operated group

rats in control group showed poor cardiac function(

0.05)

significantly increased expression of XO (

0.05)

increased BNP content and MDA level(

0.05)

significant decrease of the activity of mitochondrial complexⅠ

Ⅲ and Ⅳ（

0.05）

HE staining revealed obvious myocardial tissue edema and obviously increased iNOS expression (

0.05) in control group

which revealed that the CHF model had been set up successfully. While compared with control group

rats in ALLO treatment group showed better cardiac function(

0.05)

decreased XO expression (

0.05) and BNP content and MDA level (

0.05)

increased activity of mitochondrial complexⅠ

Ⅲ and Ⅳ(

0.05)

alleviated tissue loosening and edema (

0.05)

markedly inhibited iNOS expression (

0.05). Conclusion CHF model is set up successfully

and ALLO can improve chronic heart failure rats cardiac function. The mechanism maybe associated with

its antioxidant role．

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