Protective effects of curcumin on acute lung injury induced by LPS in rats

陈永锋; 周向东; 杨和平

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Protective effects of curcumin on acute lung injury induced by LPS in rats

更新时间：2026-01-09

- Protective effects of curcumin on acute lung injury induced by LPS in rats
- Journal of Army Medical University Vol. 36, Issue 14, Pages: 1492-1495(2014)
- 作者机构：
- 作者简介：
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- DOI：
  CLC： R285.5;R363.21;R563.05
- Published：2014
- 稿件说明：
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陈永锋,周向东,杨和平.姜黄素对内毒素诱导的急性肺损伤的保护作用及机制研究[J].陆军军医大学学报(原第三军医大学学报),2014,36(14):1492-1495.

　Chen yongfeng,Zhou Xiangdong,Yang Heping.Protective effects of curcumin on acute lung injury induced by LPS in rats[J].J Amry Med Univ (J Third Mil Med Univ),2014,36(14):1492-1495.
陈永锋,周向东,杨和平.姜黄素对内毒素诱导的急性肺损伤的保护作用及机制研究[J].陆军军医大学学报(原第三军医大学学报),2014,36(14):1492-1495. DOI：

　Chen yongfeng,Zhou Xiangdong,Yang Heping.Protective effects of curcumin on acute lung injury induced by LPS in rats[J].J Amry Med Univ (J Third Mil Med Univ),2014,36(14):1492-1495. DOI：

摘要

目的探究姜黄素在内毒素（LPS）诱导大鼠急性肺损伤（ acute lung injury，ALI）中的保护作用，并明确其作用机制。方法 80只健康雄性12周龄SD 大鼠，体质量（200±25）g，由第三军医大学实验动物中心提供。随机分为：①正常对照组：3 mL/kg生理盐水腹腔注射；②溶媒组： 1 mL/kg DMSO和2 mL/kg生理盐水腹腔注射；③模型组： 5 mg/kg LPS腹腔注射；④姜黄素组：先注射5 mg/kg LPS，30 min后注射200 mg/kg姜黄素腹腔注射。每组20只。分别于0、6、12、24 h选取5只大鼠行血气分析，Western blot检测肺组织MMP2、MMP9、ELISA检测IL-6和TNF-α的表达水平以及MPO活性。结果正常对照、溶媒组大鼠组内和组间PaO

、PaO

/FiO

、MMP2、MMP9、IL-6和TNF-α的表达水平以及MPO活性比较均无显著性差异（

0.05）。与0 h比较，模型组、姜黄素组在6、12、24等3个时点PaO

、PaO

/FiO

显著降低（

0.05），MMP2、MMP9、IL-6和TNF-α的表达水平以及MPO活性显著升高（

0.05）。与正常对照组相比，模型组、姜黄素组PaO

、PaO

/FiO

均显著降低（

0.05），MMP2、MMP9、IL-6和TNF-α的表达水平以及MPO活性显著升高（

0.05）。与模型组相比，姜黄素组PaO

、PaO

/FiO

在6、12、24 h均显著升高（P0.05），MMP2、MMP9、IL-6和TNF-α的表达水平以及MPO活性显著降低（

0.05）。结论姜黄素能有效减轻由LPS诱导的ALI，其机制可能与下调MMP2和MMP9蛋白的表达、进而减少肺组织中性粒细胞的聚集和活化、最终显著减轻肺组织炎症反应有关。

Abstract

Objective To observe the protective effect of curcumin on acute lung injury induced by LPS in rats and further to explore th

e molecular mechanism. Methods Eighty 12-week-old SD rats weighing 200±25 g

aged 12 weeks

purchased from the Experimental Animal Center of the Third Military Medical University were randomized into 4 groups and treated as follows: control group were intraperitoneal injected with saline (3 mL/kg)

the solvent group was injected with DMSO (1 mL/kg) in half an hour after intraperitoneal injection of saline (2 mL/kg)

model group was injected with 5 mg/kg LPS

and curcumin treatment group was injected with curcumin 200 mg/kg in half an hour after intraperitoneal injection of LPS. Arterial blood samples were collected in 0， 6， 12， and 24 h after drug administration for blood gas analysis

then the lung pathology was assessed

meanwhile the expression levels of MMP2 and MMP9 were measured by Western blotting and the expression levels of IL-6 and TNF-α and the activity of MPO were determined by ELISA. Results There was no significant difference between and within control group and solvent group. Compared with 0 h

PaO

and PaO

/FiO

were significantly decreased

the expression levels of MMP2

MMP9

IL-6 and TNF-α and the activity of MPO were significantly decreased in model group and curcumin treatment group at 6

12 and 24 h (

0.05). Compared with control group and solvent group

PaO

and PaO

/FiO

were significantly decreased

the expression levels of MMP2

MMP9

IL-6 and TNF-α and the activity of MPO were significantly decreased in model group and curcumin treatment group(

0.05). compared with model group

PaO

and PaO

/FiO

were significantly increased

the expression levels of MMP2，MMP9，IL-6 and TNF-α and the activity of MPO were significantly decreased in curcumin treatment group(

0.05). Conclusion Curcumin have a protective effect on acute lung injury induced by LPS in rats，and the protective mechanisms may be related to decreasedMMP2 and MMP9 protein expre

ssion.

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