MiR-224 promotes cell migration by targeting HOXD10 in Hep3B cells

丁琛琛; 李琼; 王阁

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MiR-224 promotes cell migration by targeting HOXD10 in Hep3B cells

更新时间：2026-01-09

- MiR-224 promotes cell migration by targeting HOXD10 in Hep3B cells
- Journal of Army Medical University Vol. 36, Issue 13, Pages: 1365-1369(2014)
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  CLC： R394.3;R73-37;R735.7
- Published：2014
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丁琛琛,李琼,王阁.miRNA-224通过HOXD10影响Hep3B细胞迁移[J].陆军军医大学学报(原第三军医大学学报),2014,36(13):1365-1369.

　Ding Chenchen,Li Qiong,Wang Ge.MiR-224 promotes cell migration by targeting HOXD10 in Hep3B cells[J].J Amry Med Univ (J Third Mil Med Univ),2014,36(13):1365-1369.
丁琛琛,李琼,王阁.miRNA-224通过HOXD10影响Hep3B细胞迁移[J].陆军军医大学学报(原第三军医大学学报),2014,36(13):1365-1369. DOI：

　Ding Chenchen,Li Qiong,Wang Ge.MiR-224 promotes cell migration by targeting HOXD10 in Hep3B cells[J].J Amry Med Univ (J Third Mil Med Univ),2014,36(13):1365-1369. DOI：

摘要

目的探讨miR-224影响肝癌细胞迁移的可能机制。方法采用双荧光素酶实验、载体构建和Western blot实验等对预测的miR-224靶基因进行验证。qRT-PCR检测正常肝细胞株L02和肝癌细胞株Hep3B中miR-224的表达；划痕实验观察miR-224对肝癌细胞迁移的影响。结果 qRT-PCR结果显示，与正常肝细胞株L02比较，miR-224在肝癌细胞株Hep3B中明显高表达(相对表达倍数1.679 2，

0.05)；划痕实验显示，与miR-224 mimics阴性对照组（迁移率：56.43%~58.33%）以及空白组（迁移率：60.48%~66.94%）比较， miR-224 mimics组（迁移率：80.12%~82.02%）的Hep3B细胞迁移能力明显增强（

0.05），反之亦然，提示miR-224表达水平和肝癌细胞的迁移能力成正相关；双荧光素酶实验结果显示：与对照组比较， miR-224 mimics组细胞的荧光信号明显下降(

0.05)；Western blot实验结果显示：与对照组比较，上调miR-224的表达水平后HOXD10表达下降，下调其表达水平HOXD10表达上升（

0.05）。结论 miR-224通过调控靶基因HOXD10的表达，参与调节肝癌细胞的迁移。

Abstract

Objective To investigate the possible underlying mechanism that miR-224 affects the migration of liver cancer cells. Methods A luciferase reporter assay was used to confirm that the gene HOXD10 was one direct target of miR-224. qRT-PCR

Western blotting

and scratch wound migration assay were used to clarify the molecular mechanism of miR-224 in the regulation of cell migration in human hepatocellular carcinoma line Hep3B. Results qRT-PCR indicated the expression of mi

R-224 was significantly stronger in Hep3B cells than in human hepatic L02 cells (relative expression ratio: 1.679 2

0.05). Scratch wound migration assay showed that transfection of miR-224 resulted in significantly increased ratio of migration (80.12% to 82.02%

0.05) in Hep3B cells than the cells transfected by miR-224 mimic negative control (56.43% to 58.33%) and by blank vector (60.48% to 66.94%). Compared with the miR-224 negative control

the luciferase activity was significantly decreased in 293 cells with miR-224 mimic (0.633 2). miR-224 affected the migration ability of HCC via directly targeting HOXD10. Conclusion miR-224 is involved in the regulation of hepatoma cell migration via directly targeting HOXD10.

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