Effect of curcumin on expression of Wnt/β-catenin signaling pathway in neural stem cells in vitro

陈飞; 王皓香; 向鑫; 袁继超; 朱海涛; 薛兴森; 储卫华; 王程仕; 冯华; 林江凯

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Effect of curcumin on expression of Wnt/β-catenin signaling pathway in neural stem cells in vitro

更新时间：2026-01-09

- Effect of curcumin on expression of Wnt/β-catenin signaling pathway in neural stem cells in vitro
- Journal of Army Medical University Vol. 36, Issue 8, Pages: 764-768(2014)
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  CLC： R282.71;R322.8;R329.26
- Published：2014
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陈飞,王皓香,向鑫,袁继超,朱海涛,薛兴森,储卫华,王程仕,冯华,林江凯.姜黄素对神经干细胞Wnt/β-catenin信号通路表达影响的离体研究[J].陆军军医大学学报(原第三军医大学学报),2014,36(08):764-768.

　Chen Fei,Wang Haoxiang,Xiang Xin,Yuan Jichao,Zhu Haitao,Xue Xingsen,Chu Weihua,Wang Chengshi,Feng Hua,Lin Jiangkai.Effect of curcumin on expression of Wnt/β-catenin signaling pathway in neural stem cells in vitro[J].J Amry Med Univ (J Third Mil Med Univ),2014,36(08):764-768.
陈飞,王皓香,向鑫,袁继超,朱海涛,薛兴森,储卫华,王程仕,冯华,林江凯.姜黄素对神经干细胞Wnt/β-catenin信号通路表达影响的离体研究[J].陆军军医大学学报(原第三军医大学学报),2014,36(08):764-768. DOI：

　Chen Fei,Wang Haoxiang,Xiang Xin,Yuan Jichao,Zhu Haitao,Xue Xingsen,Chu Weihua,Wang Chengshi,Feng Hua,Lin Jiangkai.Effect of curcumin on expression of Wnt/β-catenin signaling pathway in neural stem cells in vitro[J].J Amry Med Univ (J Third Mil Med Univ),2014,36(08):764-768. DOI：

摘要

目的探讨姜黄素对神经干细胞内Wnt/β-catenin信号通路的影响。方法选择孕14.5 d的SD大鼠，取胚鼠大脑前端2/3部分组织，提取神经干细胞。培养并传代3次时，进行细胞免疫荧光学鉴定；同时换用只添加2% B27的Neurobasal培养基连续培养细胞，并设置空白对照组、IWR-1（Wnt信号通路特异性阻断剂10 μmol/L）处理组、姜黄素500 nmol/L 组、IWR-1+姜黄素500 nmol/L组（先添加IWR-1，20min后再加入姜黄素）、DMSO组。细胞培养3 d时，采用Western blot方法检测各组wnt3a、β-catenin蛋白表达水平变化，采用RT-PCR方法检测各组wnt3a、β-catenin的mRNA表达量变化。结果 Western blot结果显示：对培养第3代的细胞干预3 d后，姜黄素组wnt3a、β-catenin表达量较其他各组明显升高（

0.05），IWR-1处理后其表达水平明显降低（

0.05）；姜黄素联合IWR-1处理细胞后wnt3a、β-catenin表达量明显减少（

0.05）；DMSO组表达量接近空白对照组。RT-PCR结果显示：与空白对照组比较，姜黄素组wnt3a及β-catenin基因表达水平明显上调（

0.05），而IWR-1组wnt3a及β-catenin基因表达水平明显下降（

0.05），总体变化趋势与Western blot结果一致。结论初步证明姜黄素可激活Wnt信号通路，由此推测姜黄素与经典的Wnt/β-catenin信号通路密切相关。

Abstract

Objective To investigate the effect of curcumin on expression of Wnt/β-catenin signaling pathway in neural stem cells in vitro. Methods Neural stem cells were isolated from anterior two-thirds brains of fetal Sprague-Dawley (SD) rats (14.5 d after pregnancy). The neural stem cells were passaged for 3 times by half media repla

cement and identified by cellular immunofluorescence assay. After 3 passages

cells were seeded in the media without bFGF or EGF. Then

cells were treated in different groups: control group

IWR-1 (a specific inhibitor to Wnt signaling pathway

10 μmol/L) group

500 nmol/L curcumin group

IWR-1+ curcumin 500 nmol/L group and DMSO group. We measured Wnt3a and β-catenin expression levels by Western blotting and RT-PCR separately. Results Western blotting results showed that Wnt3a and β-catenin expression levels had significant increases in the curcumin group (

0.05)

but were decreased in the IWR-1 group and IWR-1+curcumin group (

0.05). RT-PCR results showed that the expression levels of Wnt3a and β-catenin were increased in the curcumin group but decreased in the IWR-1 group (

0.05)

showing similar change pattern to the Western blot results. Conclusion Curcumin activates the classic Wnt/β-catenin signaling pathway in neural stem cells.

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