Distribution and expression profile of SDF-1 and its receptor CXCR4 in rat retinas after optic nerve injury

叶茂; 李依孺; 徐海伟; 何建荣; 彭艳丽; 吴楠; 王一

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Distribution and expression profile of SDF-1 and its receptor CXCR4 in rat retinas after optic nerve injury

更新时间：2026-01-09

- Distribution and expression profile of SDF-1 and its receptor CXCR4 in rat retinas after optic nerve injury
- Journal of Army Medical University Vol. 36, Issue 7, Pages: 645-649(2014)
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  CLC： R339.146;R341;R774.6
- Published：2014
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叶茂,李依孺,徐海伟,何建荣,彭艳丽,吴楠,王一.视神经损伤后SDF-1及其受体CXCR4在大鼠视网膜中的分布和表达规律[J].陆军军医大学学报(原第三军医大学学报),2014,36(07):645-649.

　Ye Mao,Li Yiru,Xu Haiwei,He Jianrong,Peng Yanli,Wu Nan,Wang Yi.Distribution and expression profile of SDF-1 and its receptor CXCR4 in rat retinas after optic nerve injury[J].J Amry Med Univ (J Third Mil Med Univ),2014,36(07):645-649.
叶茂,李依孺,徐海伟,何建荣,彭艳丽,吴楠,王一.视神经损伤后SDF-1及其受体CXCR4在大鼠视网膜中的分布和表达规律[J].陆军军医大学学报(原第三军医大学学报),2014,36(07):645-649. DOI：

　Ye Mao,Li Yiru,Xu Haiwei,He Jianrong,Peng Yanli,Wu Nan,Wang Yi.Distribution and expression profile of SDF-1 and its receptor CXCR4 in rat retinas after optic nerve injury[J].J Amry Med Univ (J Third Mil Med Univ),2014,36(07):645-649. DOI：

摘要

目的观察Long Evans大鼠视神经损伤后不同时间基质细胞衍生因子-1（stromal cell-derived factor-1，SDF-1）及其受体CXCR4在视网膜中的分布及表达变化。方法将21只成年Long Evans大鼠分为正常组和视神经损伤后1、3、5、7、15 d及30 d组，每组3只，采用免疫荧光染色和免疫印迹法(Western blot)检测各组大鼠视网膜SDF-1、CXCR4的分布及表达变化。结果免疫荧光染色发现视神经钳夹伤大鼠SDF-1主要定位表达于视网膜内、外界膜之间的星形胶质细胞及Müller细胞，并与GFAP共表达，SDF-1阳性细胞数量随视神经损伤时间延长先增多后减少，伤后5 d达高峰。CXCR4主要表达于视网膜神经节细胞层、外核层及内核层；Western blot证实视网膜SDF-1的水平随视神经损伤出现先升高后降低的趋势，与正常组相比，视神经损伤组第1、3、5、7天水平显著升高（

0.05）。而CXCR4的表达在视神经损伤后各时间点无明显改变（

0.05）。结论视神经损伤后，视网膜星形胶质细胞及Müller细胞中的SDF-1表达先增强后减弱，且与视神经损伤后胶质活化相关。

Abstract

Objective To observe the distributions and expression profiles of stromal cell-derived factor-1 (SDF-1) and its receptor CXCR4 in the retinas during repair period after partial optic nerve injury in Long Evans rats. Methods A total of 21 adult rats were randomly and equally divided into 7 groups

normal group

the injury group

and the groups in 1

15 and 30 d after optic nerve crushing. Immunofluorescence assay and Western blotting was applied to detect the location and the q

uantity of SDF-1 and CXCR4 expression in the retina. Results Immunofluorescence staining revealed that the expression of SDF-1 was co-localized in the activated GFAP-positive astrocytes and Müller cells between the pre-retinal and post-retinal membrane. The amount of SDF-1 positive cells were in a trend of increasing to decreasing with the injury time prolonging

and reached a summit in 5 d after injury. CXCR4 staining was mainly present in the ganglion cell layer

inner nuclear layer and outer nuclear layer of the retina. Western blotting revealed that the expression of SDF-1 protein was increased at the first day and then decreased at the 7th day after injury. The expression of SDF-1 in 1

5 and 7 d after optic nerve injuring was increased significantly than normal group (

0.05). While

the expression of CXCR4 protein had no change after injury (

0.05). Conclusion The quantity of SDF-1 is increased first and then decreased in the astrocytes and Müller cells after optic nerve injuring

which is related to the activation of glial cells after injury.

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