Objective      To investigate the expression pattern of adenosine-3-phospho-5-phosphosulfate transporter 1 (PAPST1) in medulloblastoma (MB)

and its role in proliferation of MB cells.       Methods      The expression of PAPST1 was examined by qRT-PCR in 25 MB tumor samples

1 primary cultured MB cells (pri-MB)

3 MB cell lines (DAOY

D341 and ONS-76) and the normal cerebellums. The distribution of PAPST1 protein was stained by immunohistochemical technique. siRNA technique was applied in DAOY cells to knockdown the expression PAPST1 at mRNA and protein levels

which was confirmed by qRT-PCR and Western blot analysis. CCK-8 assay was carried out to determine the proliferation activity of DAOY cells after PAPST1 knockdown.        Results      qRT-PCR revealed 2 folds up-regulation in 18/25 (72%) MB samples and in DAOY and ONS-76 MB cell lines

compared with normal human cerebellum. Immunohistochemical staining revealed that the PAPST1 protein was located in the cytoplasm

and the stronger expression of PAPST1 in MB tumor tissues. The results of qRT-PCR and Western blotting showed a significant knockdown of PAPST1 at mRNA and protein levels in DAOY MB cells

and the CCK-8 assay revealed that cell proliferation was inhibited by siRNA technique.       Conclusion      High expression of PAPST1 is a common event in MB

and down-regulation of PAPST1 can induce proliferation inhibition in MB cells.