Rapamycin-induced autophagy promotes HBV replication

李霞; 黄文娟; 朱素菲; 陈维贤

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Rapamycin-induced autophagy promotes HBV replication

更新时间：2026-01-09

- Rapamycin-induced autophagy promotes HBV replication
- Journal of Army Medical University Vol. 36, Issue 12, Pages: 1233-1236(2014)
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  CLC： R373.21;R392.32;R976
- Published：2014
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李霞,黄文娟,朱素菲,陈维贤.雷帕霉素诱导细胞自噬促进乙型肝炎病毒的复制[J].陆军军医大学学报(原第三军医大学学报),2014,36(12):1233-1236.

　Li Xia,Huang Wenjuan,Zhu Sufei,Chen Weixian.Rapamycin-induced autophagy promotes HBV replication[J].J Amry Med Univ (J Third Mil Med Univ),2014,36(12):1233-1236.
李霞,黄文娟,朱素菲,陈维贤.雷帕霉素诱导细胞自噬促进乙型肝炎病毒的复制[J].陆军军医大学学报(原第三军医大学学报),2014,36(12):1233-1236. DOI：

　Li Xia,Huang Wenjuan,Zhu Sufei,Chen Weixian.Rapamycin-induced autophagy promotes HBV replication[J].J Amry Med Univ (J Third Mil Med Univ),2014,36(12):1233-1236. DOI：

摘要

目的研究雷帕霉素诱导细胞产生自噬对乙型肝炎病毒（hepatitis B virus，HBV)复制的影响。方法通过雷帕霉素诱导人肝癌细胞HepG2 2.15产生自噬，荧光显微镜和透射电镜下观察自噬小体形成，Western blot检测诱导后HepG2 2.15细胞自噬标志蛋白LC3-II/LC3-I的表达水平；荧光定量PCR检测HBV mRNA水平；加入自噬抑制剂3-甲基腺嘌呤（3-MA），Western blot检测自噬抑制后LC3-II/LC3-I的表达水平，酶联免疫吸附试验(ELISA)检测乙肝病毒表面抗原（HBsAg）的表达，Southern blot检测HBV DNA水平，验证HBV复制情况。结果雷帕霉素诱导后，HepG2 2.15细胞在荧光显微镜和透射电镜下观察到自噬小体的形成，LC3-II/LC3-I比值显著上升（

0.05）；细胞诱导自噬后HBsAg和HBV mRNA显著增加（P0.05），而加入自噬抑制剂3-MA后，HBsAg和 HBV DNA均显著减少（

0.05）。结论雷帕霉素可能通过诱导细胞自噬促进HBV的复制。

Abstract

Objective To determine the effect of autophagy induced by rapamycin on hepatitis B virus (HBV) replication. Methods Human liver cancer HepG2 2.15 cells were treated with rapamycin to induce autophagy. Fluorescence microscopy and transmission electron microscopy (TEM) were used to observe the autophagosomes. Western blotting was performed to analyze the expression level of autophagy marker protein LC3-II/LC3-I. Fluorescence quantitative RT-PCR was used to detect HBV mRNA level. After adding the autophagy inhibitor 3-methyl adenine (3-MA)

Western blotting was employed to detect the expression

level of LC3-II/LC3-I after autophagy inhibition. Enzyme-linked immunosorbent assay (ELISA) was used to detect the expression of HBV surface antigen (HBsAg). The level of HBV DNA was evaluated by Southern blotting. Results In the HepG2 2.15 cells treated by rapamycin

autophagosomes were observed with fluorescence microscopy and TEM

while the expression level of LC3-II/LC3-I was significantly increased (

0.05). The expression of HBsAg and HBV mRNA was increased significantly (

0.05) after induction of autophagy. The expression of HBsAg and HBV DNA was significantly reduced (

0.05) after adding autophagy inhibitor 3-MA. Conclusion Rapamycin may promote HBV replication by inducing cell autophagy.

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