Diminazene aceturate ameliorates endotoxin-induced uveitis in mice

邱一果; 杨红霞; 杨培增; 雷博

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Diminazene aceturate ameliorates endotoxin-induced uveitis in mice

更新时间：2026-01-09

- Diminazene aceturate ameliorates endotoxin-induced uveitis in mice
- Journal of Army Medical University Vol. 35, Issue 20, Pages: 2195-2198(2013)
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  CLC： R-332;R773.905;R978.6
- Published：2013
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邱一果,杨红霞,杨培增,雷博.三氮脒减轻内毒素诱导性小鼠葡萄膜炎的研究[J].陆军军医大学学报(原第三军医大学学报),2013,35(20):2195-2198.

　Qiu Yiguo,Yang Hongxia,Yang Peizeng,Lei Bo.Diminazene aceturate ameliorates endotoxin-induced uveitis in mice[J].J Amry Med Univ (J Third Mil Med Univ),2013,35(20):2195-2198.
邱一果,杨红霞,杨培增,雷博.三氮脒减轻内毒素诱导性小鼠葡萄膜炎的研究[J].陆军军医大学学报(原第三军医大学学报),2013,35(20):2195-2198. DOI：

　Qiu Yiguo,Yang Hongxia,Yang Peizeng,Lei Bo.Diminazene aceturate ameliorates endotoxin-induced uveitis in mice[J].J Amry Med Univ (J Third Mil Med Univ),2013,35(20):2195-2198. DOI：

摘要

目的探讨三氮脒（diminazene aceturate，DIZE）是否能减轻内毒素诱导性小鼠葡萄膜炎。方法连续给予一般情况良好、体质量16~20 g的雌性BALB/c小鼠腹腔内注射DIZE (60 mg/kg) 2 d后，将125 ng脂多糖（lipopolysaccharides

LPS）注射入小鼠玻璃体内建立内毒素诱导性葡萄膜炎模型。分别于注射LPS后12、24 h观察小鼠眼前房炎症情况并进行临床评分。第24小时摘除眼球，石蜡切片HE染色观察小鼠眼部形态学变化。用Real-time PCR检测小鼠眼内炎症细胞因子表达。结果在注射LPS 12 h后DIZE对眼前房炎症出现明显的保护作用。24 h时DIZE+LPS组的临床评分[(1.75±0.96) 分

]

明显低于生理盐水+LPS组[(4.00±1.22) 分

]

（

0.01）；HE染色结果显示DIZE+LPS组小鼠玻璃体内渗出的炎症细胞数明显少于生理盐水+LPS组，但各组小鼠视网膜结构和层次无明显改变。DIZE+LPS组的小鼠眼内细胞间黏附分子-1（intercellular adhesion molecule-1

ICAM-1)、TNF-α、单核细胞趋化蛋白（monocyte chemoattractant protein-1

MCP-1）、IL-6的mRNA水平明显低于生理盐水+LPS组（

0.01）。结论 DIZE在LPS引起的内毒素诱导性小鼠葡萄膜炎的眼部炎症中起保护作用

有望成为一种治疗葡萄膜炎的新药。

Abstract

Objective To investigate whether diminazene aceturate (DIZE) protects mice from endotoxin-induced uveitis (EIU). Methods Female BALB/c mice received intraperitoneal injection of DIZE (60 mg/kg) for 2 d prior to intravitreous injection of lipopolysaccharides (LPS

125 ng). The anterior segments of the mice were examined and clinical scores were determined at 12 and 24 h after LPS injection. Morphology change was

evaluated with a light microscope at 24 hours after LPS injection. The mRNA levels of inflammatory cytokines in the eyes were measured by real-time PCR. Results At the 24th hour

the clinical score of mice treated with DIZE (1.75±0.96) was significantly lower than that of the saline-injected controls (4.00±1.22

0.01). Histological examination showed the total infiltrating inflammatory cells remarkably reduced in DIZE-treated eyes. The mRNA levels of ICAM-1

TNF-α

MCP-1

and IL-6 were significantly reduced in DIZE-treated eyes(

0.01). Conclusion DIZE protects retinal structure and reduces infiltrating inflammatory cells in LPS-induced EIU mouse model. These results support that DIZE may provide a novel therapeutic strategy for uveitis.

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