Effects of Aβ<sub>25-35</sub> on intracellular Ca<sup>2+</sup> concentration and autophagy in PC12 cells

陆蔚天; 孙善全; 黄娟; 陈臻; 甘胜伟; 徐进

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Effects of Aβ_25-35 on intracellular Ca²⁺ concentration and autophagy in PC12 cells

更新时间：2026-01-09

- Effects of Aβ_25-35 on intracellular Ca²⁺ concentration and autophagy in PC12 cells
- Journal of Army Medical University Vol. 36, Issue 4, Pages: 355-359(2014)
- 作者机构：
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- DOI：
  CLC： R329-33;R329.25;R341
- Published：2014
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陆蔚天,孙善全,黄娟,陈臻,甘胜伟,徐进.Aβ25-35诱导PC12细胞内Ca2+浓度升高和自噬发生[J].陆军军医大学学报(原第三军医大学学报),2014,36(04):355-359.

　Lu Weitian,Sun Shanquan,Huang Juan,Chen Zhen,Gan Shengwei,Xu Jin.Effects of Aβ25-35 on intracellular Ca2+ concentration and autophagy in PC12 cells[J].J Amry Med Univ (J Third Mil Med Univ),2014,36(04):355-359.
陆蔚天,孙善全,黄娟,陈臻,甘胜伟,徐进.Aβ25-35诱导PC12细胞内Ca2+浓度升高和自噬发生[J].陆军军医大学学报(原第三军医大学学报),2014,36(04):355-359. DOI：

　Lu Weitian,Sun Shanquan,Huang Juan,Chen Zhen,Gan Shengwei,Xu Jin.Effects of Aβ25-35 on intracellular Ca2+ concentration and autophagy in PC12 cells[J].J Amry Med Univ (J Third Mil Med Univ),2014,36(04):355-359. DOI：

摘要

目的研究Aβ

25-35

对PC12细胞内Ca

浓度和自噬发生的影响。方法培养PC12细胞，分别用0、5、10 μmol/L Aβ

25-35

处理24 h，用MTT法测定细胞存活率，流式细胞仪检测细胞内Ca

浓度，单丹磺酰戊二胺（monodansylcadaverine

MDC）染色观察自噬泡，共聚焦显微镜检测LC3-Ⅱ表达，透射电镜观察细胞超微结构。结果 5、10 μmol/L Aβ

25-35

处理24 h后，PC12细胞存活率分别为（64.6±2.3）%和（41.3±4.1）%，与对照组相比显著下降（P0.05），细胞内游离Ca

浓度升高（P0.05）。与此同时，随着Aβ

25-35

浓度升高，显微镜下可见细胞皱缩、变圆，与对照组相比，细胞内MDC阳性颗粒增多，LC3-Ⅱ荧光强度增强（P0.05），与Aβ

25-35

呈浓度依赖关系。电镜下可见单（双）层膜样自噬体。结论 Aβ

25-35

诱导PC12细胞发生自噬呈浓度依赖关系，其发生可能与细胞内游离Ca

浓度升高有关。

Abstract

Objective To study the effects of Aβ

25-35

on intracellular Ca

concentration and autophagy in PC12 cells. Methods PC12 cells were treated with 0

and 10 μmol/L Aβ

25-35

for 24 h. Cell viability was detected by MTT assay. The intracellular Ca

concentration was measured by flow cytometry. The autophagic vacuoles were observed by monodansylcadaverine (MDC) fluorescent staining. The expression of LC3-Ⅱ was

examined by fluorescent staining. The ultrastructure of cells were observed by transmission electronic microscopy. Results The viability of PC12 cells after incubation with 5 and 10 μmol/L Aβ

25-35

was (64.6±2.3)% and (41.3±4.1)%

respectively

which were significantly decreased as compared to the control group (P0.05). The intracellular Ca

concentrations of the PC12 cells after incubation with 5 and 10 μmol/L Aβ

25-35

were significantly higher than that of the control group (P0.05). Meanwhile

it could be detected that the cells after Aβ

25-35

treatment became shrink and round. The autophagic vacuoles stained with MDC in Aβ

25-35

-treated groups were significantly more than the in control group (P0.05). The expression level of LC3-Ⅱ was elevated after treated with Aβ

25-35

(P0.05) in a dose-dependent manner. Autophagosomes were observed after Aβ

25-35

treatment. Conclusion Aβ

25-35

may induce autophagy in PC12 cells in a dose-dependent manner. The mechanism might be related to the increase of intracellular Ca

concentration.

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Effects of Aβ25-35 on intracellular Ca2+ concentration and autophagy in PC12 cells

DOI：

摘要

Abstract

关键词

Keywords

references

Effects of Aβ_25-35 on intracellular Ca²⁺ concentration and autophagy in PC12 cells