Effects of Roux-en-Y gastric bypass on level of aquaporin-9 in type 2 diabetes rats

蒙长远; 陈志雄; 官文飞; 母松; 骆赞; 汪志红; 张军

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Effects of Roux-en-Y gastric bypass on level of aquaporin-9 in type 2 diabetes rats

更新时间：2026-01-09

- Effects of Roux-en-Y gastric bypass on level of aquaporin-9 in type 2 diabetes rats
- Journal of Army Medical University Vol. 36, Issue 3, Pages: 257-261(2014)
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  CLC： R363;R587.1;R656.61
- Published：2014
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蒙长远,陈志雄,官文飞,母松,骆赞,汪志红,张军.Roux-en-Y胃转流术对2型糖尿病大鼠肝脏AQP9的影响[J].陆军军医大学学报(原第三军医大学学报),2014,36(03):257-261.

　Meng Changyuan,Chen Zhixiong,Guan Wenfei,Mu Song,Luo Zan,Wang Zhihong,Zhang Jun.Effects of Roux-en-Y gastric bypass on level of aquaporin-9 in type 2 diabetes rats[J].J Amry Med Univ (J Third Mil Med Univ),2014,36(03):257-261.
蒙长远,陈志雄,官文飞,母松,骆赞,汪志红,张军.Roux-en-Y胃转流术对2型糖尿病大鼠肝脏AQP9的影响[J].陆军军医大学学报(原第三军医大学学报),2014,36(03):257-261. DOI：

　Meng Changyuan,Chen Zhixiong,Guan Wenfei,Mu Song,Luo Zan,Wang Zhihong,Zhang Jun.Effects of Roux-en-Y gastric bypass on level of aquaporin-9 in type 2 diabetes rats[J].J Amry Med Univ (J Third Mil Med Univ),2014,36(03):257-261. DOI：

摘要

目的研究Roux-en-Y胃转流术（Roux-en-Y gastric bypass，RYGB）对2型糖尿病（type 2 diabetes mellitus

T2DM）大鼠肝脏水通道蛋白-9（aquaporin-9

AQP9）表达的影响，初步探讨RYGB改善T2DM的机制。方法 SD大鼠50只，按随机数字表法选取40只建立T2MD模型，按干预方式分为糖尿病组（B组，n=10）、糖尿病假手术组（C组，n=10）、糖尿病手术组（D组，n=10），另10只大鼠作为正常对照组（A组）。检测各组大鼠术前和术后5、10、15、20 d的空腹血糖（fasting blood glucose

FBG）、血清胰岛素及胰高血糖素样肽-1（glucagon-like peptide-1，GLP-1）水平。于术后20 d处死各组大鼠，分别用免疫荧光染色和Western blot检测肝脏组织AQP9的表达情况。结果 D组大鼠术后5 d空腹血糖由术前（23.4±1.6）mmol/L降至（8.7±0.8）mmol/L，术后20 d空腹血糖为（7.8±0.8）mmol/L；术后空腹胰岛素较术前无显著变化（

0.05）；术后5 d空腹血清GLP-1由术前（7.7±0.3）pmol/L升至（16.8±0.9）pmol/L，术后20 d为（20.3±0.7）pmol/L；术后20 d，D组大鼠肝脏组织中AQP9的相对表达量(0.21±0.02)明显低于B、C组[分别为（0.54±0.11）、（0.58±0.09），

0.05

]

。结论 RYGB可显著降低T2MD大鼠肝脏中AQP9的表达，降低空腹血糖。

Abstract

Objective To investigate the effects of Roux-en-Y gastric bypass (RYGB) on aquaporin-9 (AQP9) expression in the liver of rats with type 2 diabetes mellitus (T2DM)

and to explore the mechanism for RYGB ameliorating T2DM. Methods Forty SD rats randomly chosen from 50 were divided into three groups: diabetes group (group B

n=10)

diabetes

sham operation group (group C

n=10)

and diabetes operation group (group D

n=10). The left 10 rats were used as normal control group (group A). The levels of fasting blood glucose (FBG)

insulin and glucagon-like peptide 1 (GLP-1) were measured before operation and at 5

15 and 20 d after operation. All the rats were sacrificed at 20 d after operation and the expression of AQP9 in rat livers was determined by immunofluorescence assay and Western blotting. Results As for the group D

the level of FBG decreased from (23.4±1.6) mmol/L (before operation) to (8.7±0.8) mmol/L and (7.8±0.8) mmol/L at 5 d and 20 d after operation

respectively. The level of fasting insulin did not change significantly. The level of fasting GLP-1 increased from (7.7±0.3) pmol/L (before operation) to (16.8±0.9) pmol/L and (20.3±0.7) pmol/L at 5 d and 20 d after operation

respectively. The relative expression level of AQP9 was significantly lower in the group D (0.21±0.02) than in the group B (0.54±0.11) and group C (0.58±0.09) after operation (

0.05). Conclusion RYGB can significantly reduce the levels of FBG and AQP9 expression in the liver of rats with T2DM.

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