Objective        To determine the effects of siRNA interfering metastasis-associated in colon cancer 1 (MACC1) on the extracorporeal proliferation

migration and invasion of the esophageal squamous cell carcinoma (ESCC) cell line TE-1.       Methods        The special MACC1-siRNA sequence was chemically synthesize

and then transfected into the T

E-1 cells with lipofectamine

TM

2000. RT-quantity PCR and Western blotting were used to detect the mRNA and protein level of MACC1 in untreated TE-1 cells

lipo2000-treated cells (mock)

siRNA-nc transfected cells

and siRNA-MACC1 transfected cells. MTT assay was used to detect the proliferation of TE-1 cells. Scratching test and Transwell chamber assay were evaluated the migration of the cells.       Results      Compared with the untreated cell

mock

and siRNA-nc cells

the expression of MACC1 was obviously decreased by 71% at mRNA and 87% at protein levels. In 7 dafter transfection

MTT assay indicated that the proliferation of the transfected TE-1 cells was significantly inhibited compared with the other 3 types of cells (

P

0.05)

especially from the fourth day. Scratching test showed that there were obviously less migrated cells from the siRNA-MACC1 group than those from the control (10.6±2.2 vs 68.2±2.5

P

0.05). Transwell assay also showed the invasion ability of TE-1 cells transfected with siRNA-Macc1 was lesser than the control cells (4.2±1.92 vs 58.4±7.44

P

0.05）. But no significant difference was seen among the untreated cell

mock

and siRNA-nc cells.       Conclusion       siRNA interference of MACC1 obviously down-regulates the molecule at mRNA and protein levels in TE-1 cells

and results in significant inhibition in the proliferation

migration and invasion of the cells. Therefore

MACC1 might be involved in the growth

migration and invasion of ESCC.