Effect of SIRT2 knock-down on migration and invasion in hepatocellular carcinoma cells

宋春丽; 任吉华; 冉龙宽; 李宛蔚; 陈娟

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Effect of SIRT2 knock-down on migration and invasion in hepatocellular carcinoma cells

更新时间：2026-01-09

- Effect of SIRT2 knock-down on migration and invasion in hepatocellular carcinoma cells
- Journal of Army Medical University Vol. 35, Issue 24, Pages: 2634-2638(2013)
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  CLC： R394-33;R73-37;R735.7
- Published：2013
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宋春丽,任吉华,冉龙宽,李宛蔚,陈娟.SIRT2沉默对肝癌细胞的迁移和侵袭能力的影响[J].陆军军医大学学报(原第三军医大学学报),2013,35(24 ):2634-2638.　Song Chunli,Ren Jihua,Ran Longkuan,Li Wanwei,Chen Juan.Effect of SIRT2 knock-down on migration and invasion in hepatocellular carcinoma cells[J].J Amry Med Univ (J Third Mil Med Univ),2013,35(24 ):2634-2638.

宋春丽, 任吉华, 冉龙宽, et al. Effect of SIRT2 knock-down on migration and invasion in hepatocellular carcinoma cells[J]. Journal of Army Medical University, 2013, 35(24): 2634-2638.
宋春丽,任吉华,冉龙宽,李宛蔚,陈娟.SIRT2沉默对肝癌细胞的迁移和侵袭能力的影响[J].陆军军医大学学报(原第三军医大学学报),2013,35(24 ):2634-2638.　Song Chunli,Ren Jihua,Ran Longkuan,Li Wanwei,Chen Juan.Effect of SIRT2 knock-down on migration and invasion in hepatocellular carcinoma cells[J].J Amry Med Univ (J Third Mil Med Univ),2013,35(24 ):2634-2638. DOI：

宋春丽, 任吉华, 冉龙宽, et al. Effect of SIRT2 knock-down on migration and invasion in hepatocellular carcinoma cells[J]. Journal of Army Medical University, 2013, 35(24): 2634-2638. DOI：

摘要

目的探讨慢病毒介导的沉默信息调节因子2(silent information regulator2，SIRT2)基因沉默对肝癌细胞的迁移和侵袭能力的影响。方法 Western blot分析SIRT2在多个肝癌细胞系和永生化肝细胞中的表达，通过慢病毒介导的shRNA 干扰技术靶向沉默SIRT2的表达，并通过Real-time PCR 和Western blot验证SIRT2基因的沉默效果。细胞迁移及侵袭实验检测SIRT2基因沉默对肝癌细胞迁移和侵袭能力的影响，Western blot 检测上皮细胞间充质转化（epithelial-mesenchymal transition

EMT)指标蛋白(E-cadherin、α-catenin、α-SMA、N-cadherin)的表达。结果 SIRT2在多个肝癌细胞系中蛋白表达水平明显上调；慢病毒介导的shRNA 能显著抑制细胞SIRT2的表达水平(P0.05)。SIRT2基因沉默能抑制肝癌细胞的迁移和侵袭能力(P0.01)，并使上皮属性蛋白E-cadherin、α-catenin表达水平升高，使间质属性蛋白α-SMA、N-cadherin表达水平降低。结论 SIRT2基因沉默可以通过抑制肝癌细胞上皮细胞间充质转化从而抑制肝癌细胞的迁移和侵袭能力。

Abstract

Objective To determine the effects of lentivirus-mediated silent information regulator 2 (SIRT2) knock-down on the migration and invasion in hepatocellular carcinoma cells (HCCs). Methods The expression of SIRT2 in HCC cell lines and L02 cells (immortalized human liver cells) was analyzed by real-time PCR and Western blotting. SIRT2 expression was attenuated by lentivirus-mediated shRNA interference technology

and SIRT2 protein level was measured by Western blotting. Effect of SIRT2 on migration

and invasion of HCCs was detected by migration and invasion assay. Epithelial-mesenchymal transition (EMT) proteins were tested by Western blotting. Results SIRT2 was overexpressed in the HCC cell lines. Lentivirus-mediated shRNA interference significantly down-regulated the SIRT2 expression levels in HCCs(P0.05). Knock-down of SIRT2 suppressed the migration and invasion of HCCs (

0.01) and induced upregulation of epithelial markers (E-cadherin and α-catenin) as well as downregulation of mesenchymal markers (α-SMA and N-cadherin). Conclusion Knock-down of SIRT2 inhibits the migration and invasion in HCCs possibly through EMT.

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