In vitro chemosensitivity of cerebral glioma to chemotherapeutic drugs

苏祖禄; 苏海; 张毅; 陈皓

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In vitro chemosensitivity of cerebral glioma to chemotherapeutic drugs

更新时间：2026-01-09

- In vitro chemosensitivity of cerebral glioma to chemotherapeutic drugs
- Journal of Army Medical University Vol. 35, Issue 13, Pages: 1420-1423(2013)
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  CLC： R739.41;R965.3;R979.1
- Published：2013
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苏祖禄,苏海,张毅,陈皓.脑胶质瘤化疗药物的体外敏感性试验研究[J].陆军军医大学学报(原第三军医大学学报),2013,35(13):1420-1423.

　Su Zulu,Su Hai,Zhang Yi,Chen Hao.In vitro chemosensitivity of cerebral glioma to chemotherapeutic drugs[J].J Amry Med Univ (J Third Mil Med Univ),2013,35(13):1420-1423.
苏祖禄,苏海,张毅,陈皓.脑胶质瘤化疗药物的体外敏感性试验研究[J].陆军军医大学学报(原第三军医大学学报),2013,35(13):1420-1423. DOI：

　Su Zulu,Su Hai,Zhang Yi,Chen Hao.In vitro chemosensitivity of cerebral glioma to chemotherapeutic drugs[J].J Amry Med Univ (J Third Mil Med Univ),2013,35(13):1420-1423. DOI：

摘要

目的探讨采用MTT法检测肿瘤化疗药对脑胶质瘤细胞的敏感性。方法分离临床实体瘤标本（原代脑胶质瘤组织）制备单细胞悬液，包括U87细胞进行体外培养，将临床上常用的8种肿瘤化疗药5-氟尿嘧啶(5-Fu)、卡铂(CBP)、顺铂(DDP)、阿霉素(DOX)、甲氨蝶呤(MTX)、依托泊苷(VP-16)、丝裂霉素（MMC）、长春新碱（VCR）配成3种不同的浓度（5-Fu的终浓度为250、500、750 μg/mL，其他肿瘤化疗药终浓度50、100、150 μg/mL），采用MTT法检测，计算各肿瘤化疗药物对肿瘤细胞的抑制率。结果 8种肿瘤化疗药对原代脑胶质瘤的抑制效率依次为：ADM＞MMC＞DDP＞VCR＞5-Fu＞VP-16＞CBP＞MTX；其中ADM的敏感度最高（抑制率95.54%）；其次是MMC和DDP（抑制率＞60%），MTX最不敏感（11.29%）。7种化疗药物对U87细胞株的抑制率依次为：ADM＞VP-16＞CBP＞MMC＞5-Fu＞DDP＞MTX；ADM的抑制率最高（91.19%），最不敏感的是MTX，其次是DDP（二者＜30%）。DDP对于U87细胞抑制率较低，属于耐受药，但作用于原代脑胶质瘤细胞时细胞毒作用较大，为敏感性化疗药物。结论 MTT法体外药敏试验为指导实体瘤患者的临床化疗方案提供了有效数据，可减少用药的盲目性，提高个体化治疗效果。

Abstract

Objective To evaluate the sensitivity of human cerebral glioma to chemotherapeutic drugs by MTT assay. Methods In vitro sensitivities of primary cerebral glioma cells and U87 glioma cells to eight chemotherapeutic drugs such as adriamycin (ADM)

5-fluorouracil (5-Fu)

carboplatin (CBP)

cisplatin (DDP)

methotrexate (MTX)

etoposide (VP-16)

mitomycin (MMC) and vincristine (VCR) were determined by modified MTT assay. Results In primary cerebral glioma cells

the sensitivity to the eight chemotherapeutic drugs from high to low was ADMMMCDDPVCR5-FuVP-16CBPMTX. Among those

ADM was the most effective chemotherapeutic drug to glioma cells with an inhibitory rate of 95.54%. Meanwhile

in U87 glioma cells

the sensitivity to seven chemotherapeutic drugs from high to low was ADMVP-16CBPMMC5-FuDDPMTX. ADM was the most effective chemotherapeutic drug to U87 cells with an inhibitory rate of 91.19%. U87 glioma cells were not sensitive to MTX and DDP

and the inhibitory rate was less than 30%. Conclusion MTT assay is a rapid method for screening antineoplastic agent against glioma. The glioma cells are more sensitive to ADM in vitro than to MMC

VCR

5-Fu

VP-16

CBP and MTX. Evaluation of the chemotherapeutic drugs to human glioma by MTT assay can be a helpful guidance for clinical chemotherapy

and improve the effectiveness of individualized chemotherapy.

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