Diagnostic value of whole blood gamma interferon-inducible protein 10 release assay in active pulmonary tuberculosis

罗效梅; 熊玉霞; 曹均; 王燕妮; 杨刚毅; 李伶; 廖涌

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Diagnostic value of whole blood gamma interferon-inducible protein 10 release assay in active pulmonary tuberculosis

更新时间：2026-01-09

- Diagnostic value of whole blood gamma interferon-inducible protein 10 release assay in active pulmonary tuberculosis
- Journal of Army Medical University Vol. 35, Issue 19, Pages: 2092-2094(2013)
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  CLC： R521.04;R446.112;R446.61
- Published：2013
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罗效梅,熊玉霞,曹均,王燕妮,杨刚毅,李伶,廖涌.全血γ干扰素诱导蛋白-10释放试验对活动性肺结核的诊断价值[J].陆军军医大学学报(原第三军医大学学报),2013,35(19):2092-2094.

　Luo Xiaomei,Xiong Yuxia,Cao Jun,Wang Yanni,Yang Gangyi,Li Ling,Liao Yong.Diagnostic value of whole blood gamma interferon-inducible protein 10 release assay in active pulmonary tuberculosis[J].J Amry Med Univ (J Third Mil Med Univ),2013,35(19):2092-2094.
罗效梅,熊玉霞,曹均,王燕妮,杨刚毅,李伶,廖涌.全血γ干扰素诱导蛋白-10释放试验对活动性肺结核的诊断价值[J].陆军军医大学学报(原第三军医大学学报),2013,35(19):2092-2094. DOI：

　Luo Xiaomei,Xiong Yuxia,Cao Jun,Wang Yanni,Yang Gangyi,Li Ling,Liao Yong.Diagnostic value of whole blood gamma interferon-inducible protein 10 release assay in active pulmonary tuberculosis[J].J Amry Med Univ (J Third Mil Med Univ),2013,35(19):2092-2094. DOI：

摘要

目的探讨全血γ干扰素诱导蛋白-10（IP-10）对活动性结核病的辅助诊断价值。方法检测66例活动性肺结核病患者、40例非结核呼吸疾病患者及40例健康对照者血浆中非特异性IP-10的水平；分别应用纯化的结核分枝杆菌特异性蛋白早期分泌性靶抗原-6（EAST-6）和培养液蛋白10(CFP10)体外刺激患者全血，检测活动性结核病组、非结核呼吸疾病组和健康对照组人群全血中IP-10的释放水平；绘制受试者工作特征曲线，比较非特异性IP-10和特异性IP-10对活动性肺结核的诊断效能。结果活动性结核病组患者血浆中非特异性IP-10的水平为(139.6±124.2) pg/mL，明显高于健康对照组[(33.5±17.7)pg/mL

0.05

]

；而与非结核呼吸疾病组[(88.1±73.3)pg/mL

]

无明显差别(

0.05)。经ESAT-6及CFP10诱导后，活动性结核病组患者全血IP-10释放水平为（146.0±167.1）pg/mL，显著高于非结核呼吸疾病组[(26.6±9.7)pg/mL

0.01

]

与健康对照组[(24.2±9.7)pg/mL

0.01

]

。经过ROC分析，结核特异性的IP-10诊断结核病的临界值为41.2 pg/mL，敏感度为68.2%，特异度为93.7%。特异性IP-10 ROC曲线下面积为0.905，高于非特异性IP-10(0.747

0.01)。结论 IP-10可作为活动性结核病的辅助诊断方法。采用结核特异性IP-10 较血浆非特异性IP-10能够提高活动性肺结核的诊断效能。

Abstract

Objective To determine the clinical value of interferon-inducible protein 10 (IP-10) in auxiliary diagnosis of active tuberculosis. Methods The level of non-specific IP-10 was evaluated in the plasma of 66 active tuberculosis patients

40 non-pulmonary tuberculous patients and 40 healthy controls

and the level of s

pecific IP-10 was detected in the whole blood after stimulated by purified mycobacterium tuberculosis-specific antigen ESAT6 and culture filtrate protein 10 (CFP10). Then the receiver operating characteristic (ROC) curve was drawn to determine the diagnostic value of non-specific IP-10 and specific IP-10 to detect active tuberculosis. Results The non-specific IP-10 level of the active tuberculosis group (139.6±124.2 pg/mL) was obviously higher than that of the healthy control group (33.5±17.7 pg/mL

0.05)

but had no difference with that of the non-tuberculous pulmonary disease group (88.1±73.3 pg/mL

P0.05). After stimulated by the ESAT6 and CFP10

the specific IP-10 level of active tuberculosis group (146.0±167.1 pg/mL) was significantly higher than that of non-tuberculous pulmonary disease group (26.6±9.7 pg/mL

0.01) and that of the healthy control group (24.2±9.7 pg/mL

0.01). After ROC analysis

the threshold of the specific IP-10 for diagnosis of tuberculosis was 41.1 pg/mL and its sensitivity and specificity were 70.8% and 91.8% respectively. The area under curve (AUC) of the specific IP-10 was 0.905

which was obviously higher than that of non-specific IP-10 (0.747

0.01). Conclusion The gamma interferon-inducible protein 10 release assay can be used as auxiliary diagnostic method for active tuberculosis. The diagnostic value of the specific IP-10 is better than that of non-specific IP-10.

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