Rac1 expression in ovarian cancer tissues and its role in malignant proliferation and migration of ovarian cancer cell SKOV3

彭慧娟; 张瑜; 高一萌; 罗淑娟; 刘斌; 令狐华

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Rac1 expression in ovarian cancer tissues and its role in malignant proliferation and migration of ovarian cancer cell SKOV3

更新时间：2026-01-09

- Rac1 expression in ovarian cancer tissues and its role in malignant proliferation and migration of ovarian cancer cell SKOV3
- Journal of Army Medical University Vol. 35, Issue 13, Pages: 1402-1405(2013)
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  CLC： R394.2;R730.23;R737.31
- Published：2013
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彭慧娟,张瑜,高一萌,罗淑娟,刘斌,令狐华.Rac1蛋白在卵巢癌组织中的表达及其对卵巢癌细胞株SKOV3增殖及迁移的作用[J].陆军军医大学学报(原第三军医大学学报),2013,35(13):1402-1405.

　Peng Huijuan,Zhang Yu,Gao Yimeng,Luo Shujuan,Liu Bin,Linghu Hua.Rac1 expression in ovarian cancer tissues and its role in malignant proliferation and migration of ovarian cancer cell SKOV3[J].J Amry Med Univ (J Third Mil Med Univ),2013,35(13):1402-1405.
彭慧娟,张瑜,高一萌,罗淑娟,刘斌,令狐华.Rac1蛋白在卵巢癌组织中的表达及其对卵巢癌细胞株SKOV3增殖及迁移的作用[J].陆军军医大学学报(原第三军医大学学报),2013,35(13):1402-1405. DOI：

　Peng Huijuan,Zhang Yu,Gao Yimeng,Luo Shujuan,Liu Bin,Linghu Hua.Rac1 expression in ovarian cancer tissues and its role in malignant proliferation and migration of ovarian cancer cell SKOV3[J].J Amry Med Univ (J Third Mil Med Univ),2013,35(13):1402-1405. DOI：

摘要

目的研究Rac1蛋白在人卵巢浆液性腺癌组织中的表达及其对卵巢癌细胞株SKOV3恶性增殖和迁移的影响。方法 Western blot检测人卵巢浆液性腺癌组织、卵巢良性浆液性囊腺瘤组织及卵巢正常组织中Rac1蛋白的表达水平;构建Rac1表达缺失的细胞株SKOV3-Rac1i；分别用细胞划痕实验、MTT及平板克隆实验检测SKOV3-Rac1i细胞迁移能力、细胞增殖及克隆形成能力。结果卵巢浆液性腺癌组织中Rac1蛋白表达水平显著高于卵巢良性浆液性囊腺瘤组织[(0.89±0.17) vs (0.69±0.24)，

0.05

]

和卵巢正常组织[(0.89±0.17) vs (0.72±0.12)，

0.05

]

；与亲代细胞相比，SKOV3-Rac1i细胞表现出迁移能力减弱（

0.05）和增殖能力明显受限（

0.05）。结论 Rac1在人卵巢浆液性腺癌组织中呈高表达，其可能是通过影响细胞迁移能力及细胞的增殖而促进卵巢癌细胞的恶性生物行为。

Abstract

Objective To observe the expression of Rac1 in human ovarian serous adenocarcinoma tissues and its role in the malignant proliferation and migration of ovarian cancer cell SKOV3. Methods Western blotting was applied to detect the expression of Rac1 protein in ovarian serous adenocarcinoma tissues

benign ovarian serous cystadenoma tissues and normal ovarian tissues. Stable Rac1-silencing SKOV3 cells were constructed and named as SKOV3-Rac1i. Wound healing assay was conducted to evaluate the ability of the cell migration

and MTT assay and plate

clone assay were performed to detect the proliferation rate of SKOV3-Rac1i cells. Results The expression level of Rac1 protein was significantly higher in human ovarian serous adenocarcinoma tissues than in benign ovarian serous cystadenoma tissues (0.89±0.17 vs 0.69±0.24

0.05) and normal ovarian tissues (0.89±0.17 vs 0.72±0.12

0.05). Moreover

suppressed migration and proliferation were observed in SKOV3-Rac1i cells (

0.05). Conclusion Rac1 is highly expressed in human ovarian serous adenocarcinoma tissues

and suppressed migration and proliferation of SKOV3-Rac1i cells support its contribution to malignant biological behavior of ovarian cancer cells.

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