Potassium channel tetramerisation domain containing 15 regulates preadipocyte differentiation

徐景; 赵旭; 杨莹; 徐梓辉

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Potassium channel tetramerisation domain containing 15 regulates preadipocyte differentiation

更新时间：2026-01-09

- Potassium channel tetramerisation domain containing 15 regulates preadipocyte differentiation
- Journal of Army Medical University Vol. 35, Issue 11, Pages: 1115-1118(2013)
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  CLC： R392.2;R394-33;R394.2
- Published：2013
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徐景,赵旭,杨莹,徐梓辉.KCTD15基因调控3T3-L1脂肪前体细胞分化的研究[J].陆军军医大学学报(原第三军医大学学报),2013,35(11):1115-1118.

　Xu Jing,Zhao Xu,Yang Ying,Xu Zihui.Potassium channel tetramerisation domain containing 15 regulates preadipocyte differentiation[J].J Amry Med Univ (J Third Mil Med Univ),2013,35(11):1115-1118.
徐景,赵旭,杨莹,徐梓辉.KCTD15基因调控3T3-L1脂肪前体细胞分化的研究[J].陆军军医大学学报(原第三军医大学学报),2013,35(11):1115-1118. DOI：

　Xu Jing,Zhao Xu,Yang Ying,Xu Zihui.Potassium channel tetramerisation domain containing 15 regulates preadipocyte differentiation[J].J Amry Med Univ (J Third Mil Med Univ),2013,35(11):1115-1118. DOI：

摘要

目的探讨含钾通道四聚化结构域15（KCTD15）基因在3T3-L1脂肪前体细胞分化过程中的作用。方法 ①采用半定量逆转录PCR检测在3T3-L1脂肪前体细胞分化过程中KCTD15 mRNA表达变化。②在3T3-L1脂肪前体细胞增殖早期通过RNA干扰技术靶向敲低KCTD15基因的表达，在靶向敲低KCTD15基因后的转染KCTD15 siRNA 48 h后通过半定量逆转录PCR验证KCTD15基因的敲低效果。用油红O染色法观察KCTD15敲低后3T3-L1细胞第0天和第10天的细胞形态学改变。③采用半定量逆转录PCR检测KCTD15基因敲低后PPARγ、C/EBPα、C/EBPβ、C/EBPδ成脂基因的变化。结果在3T3-L1脂肪前体细胞分化过程中，KCTD15 mRNA表达水平逐渐降低（

0.05）；KCTD15敲低能显著抑制3T3-L1脂肪前体细胞分化；KCTD15敲低后PPARγ、C/EBPα、C/EBPβ、C/EBPδ成脂基因无明显变化。结论在分化早期阶段敲低KCTD15基因能抑制3T3-L1脂肪前体细胞分化。

Abstract

Objective To study the effect of potassium channel tetramerisation domain containing 15 (KCTD15) gene on preadipocyte differentiation. Methods The expression of KCTD15 gene during 3T3-L1 preadipocyte differentiation was detected by semi-quantitative reverse transcriptase PCR. After transferring KCTD15 siRNA into the preadipocytes

the cell morphology was observed during preadipocyte differentiation by oil red O staining

and the level of triglyceride was examined by assay kit. The expression of adipogenesis genes

peroxisome proliferator-activated receptor (PPAR)γ

CCAAT/enhancer-binding protein (C/EBP)α

C/EBPβ and C/EBPδ was detected by semi-quantitative reverse transcriptase PCR. Results The expression of KCTD15 gene was decreased during 3T3-L1 cell differentiation. KCTD15 gene knockdown inhibited the differentiation and lipid accumulation of 3T3-L1 cells

and there was no significant change in the expression of PPARγ

C/EBPα

C/EBPβ and C/EBPδ. Conclusion KCTD15 gene deficiency leads to the inhibition of 3T3-L1 preadipocyte differentiation at early stage.

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