Apelin promotes proliferation and suppresses apoptosis in rat ovarian granulosa cells via PI3K/Akt signaling pathway

罗爽; 王继东; 何佳; 姚珍薇

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Apelin promotes proliferation and suppresses apoptosis in rat ovarian granulosa cells via PI3K/Akt signaling pathway

更新时间：2026-01-09

- Apelin promotes proliferation and suppresses apoptosis in rat ovarian granulosa cells via PI3K/Akt signaling pathway
- Journal of Army Medical University Vol. 35, Issue 16, Pages: 1713-1716(2013)
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  CLC： R341;R394.3;R711.75
- Published：2013
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罗爽,王继东,何佳,姚珍薇.apelin对大鼠卵巢颗粒增殖与凋亡的影响[J].陆军军医大学学报(原第三军医大学学报),2013,35(16):1713-1716.

　Luo Shuang,Wang Jidong,He Jia,Yao Zhenwei.Apelin promotes proliferation and suppresses apoptosis in rat ovarian granulosa cells via PI3K/Akt signaling pathway[J].J Amry Med Univ (J Third Mil Med Univ),2013,35(16):1713-1716.
罗爽,王继东,何佳,姚珍薇.apelin对大鼠卵巢颗粒增殖与凋亡的影响[J].陆军军医大学学报(原第三军医大学学报),2013,35(16):1713-1716. DOI：

　Luo Shuang,Wang Jidong,He Jia,Yao Zhenwei.Apelin promotes proliferation and suppresses apoptosis in rat ovarian granulosa cells via PI3K/Akt signaling pathway[J].J Amry Med Univ (J Third Mil Med Univ),2013,35(16):1713-1716. DOI：

摘要

目的探讨apelin对大鼠卵巢颗粒细胞增殖与凋亡的影响。方法用apelin 10

-8

mol/L培育SD大鼠卵巢原代颗粒细胞，小RNA干扰技术（siRNA）抑制颗粒细胞APJ表达，联合PI3K/Akt信号转导阻断剂LY294002和HIMO干预，MTT法及流式细胞术观察细胞增殖与凋亡情况；Western blot检测细胞凋亡相关信号蛋白表达。结果 MTT检测APJ-siRNA组、LY294002组、HIMO组细胞增殖率，与对照组比较差异均有统计学意义(

0.05)；与对照组比较，APJ-siRNA组、LY294002组、HIMO组颗粒细胞Bad、Bax、Foxo3a蛋白表达明显上调(

0.05)；与对照组比较，APJ-siRNA组、LY294002组、HIMO组Bc1-2蛋白表达明显下调(

0.05)。结论 ape1in通过APJ/PI3K/Akt信号通路促颗粒细胞增殖抗其凋亡。

Abstract

Objective To determine the effect of apelin on the proliferation and apoptosis in rat ovarian granulosa cells (GCs) in vitro. Methods SD rat ovarian GCs were isolated and primary cultured

and then cultured with apelin at a concentration of 10

-8

mol/L for 12

24 and 48 h respectively

and then tested by MTT assay and flow cytometry (Annexin/PI). RNA interference (RNAi) was used to down-regulate the expression of APJ in GCs. PI3K/Akt inhibitor LY294002 and HIMO were used to determine the role of the signaling pathway. Western blotting was employed to determine the expression of cell a

poptosis-related proteins. Results Apelin of 10

-8

mol/L promoted the proliferation and anti-apoptosis in GCs (

0.05). Apelin activated the phosphorylation of Akt

while APJ-siRNA

inhibitor LY294002 and HIMO abolished apelin-induced activation of Akt. Western bloting showed that apelin enhancecd the expression of Bcl-2 but suppressed the expression of Bax

Bad

and Foxo3a (

0.05). The effects were blocked by APJ-siRNA

inhibitor LY294002 and HIMO (

0.05). Conclusion Apelin promotes the proliferation and suppresses the apoptosis in GC via the PI3K/Akt signaling pathway.

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