Rock signaling pathway mediates rabbit basilar artery smooth muscle proliferation stimulated by TNF-α

安家印; 周政; 刘俊; 杨辉; 雷雪; 李正杨

您当前的位置：

首页 >

文章列表页 >

Rock signaling pathway mediates rabbit basilar artery smooth muscle proliferation stimulated by TNF-α

更新时间：2026-01-09

- Rock signaling pathway mediates rabbit basilar artery smooth muscle proliferation stimulated by TNF-α
- Journal of Army Medical University Vol. 35, Issue 18, Pages: 1952-1956(2013)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：
  CLC： R322.121;R329.26;R329.28
- Published：2013
- 稿件说明：
移动端阅览
安家印,周政,刘俊,杨辉,雷雪,李正杨.Rock信号通路参与TNF-α刺激兔基底动脉平滑肌增殖[J].陆军军医大学学报(原第三军医大学学报),2013,35(18):1952-1956.

　An Jiayin,Zhou Zheng,Liu Jun,Yang Hui,Lei Xue,Li Zhengyang.Rock signaling pathway mediates rabbit basilar artery smooth muscle proliferation stimulated by TNF-α[J].J Amry Med Univ (J Third Mil Med Univ),2013,35(18):1952-1956.
安家印,周政,刘俊,杨辉,雷雪,李正杨.Rock信号通路参与TNF-α刺激兔基底动脉平滑肌增殖[J].陆军军医大学学报(原第三军医大学学报),2013,35(18):1952-1956. DOI：

　An Jiayin,Zhou Zheng,Liu Jun,Yang Hui,Lei Xue,Li Zhengyang.Rock signaling pathway mediates rabbit basilar artery smooth muscle proliferation stimulated by TNF-α[J].J Amry Med Univ (J Third Mil Med Univ),2013,35(18):1952-1956. DOI：

摘要

目的探索Rock信号通路在肿瘤坏死因子α(tumor necrosis factor-α，TNF-α) 引起兔基底动脉血管平滑肌细胞（vascular smooth muscle cells，VSMCs）增殖中的作用。方法组织块法原代培养兔基底动脉VSMCs，TNF-α刺激VSMCs，Rho激酶(Rho kinase，Rock)抑制剂Y-27632预处理，CCK-8法检测细胞增殖变化，流式细胞仪检测细胞周期，免疫细胞化学观察增殖细胞核抗原(proliferating cell nuclear antigen，PCNA)在VSMCs的定位及表达变化，Western blot法检测细胞PCNA表达量的变化。结果细胞培养24 h后，与阴性对照组比较，20 ng/mL TNF-α处理可以显著增加VSMCs的增殖率（

0.01），PCNA蛋白表达显著增高（

0.01），S期+G

/M期细胞比例明显增加（

0.05）；加入Y-27632预处理后

与20 ng/mL TNF-α处理组相比，VSMCs的增殖率受到显著抑制（

0.01），PCNA蛋白表达明显减少（

0.05），S期+G

/M期细胞比例明显减少（

0.05）。结论 TNF-α可促进VSMCs增殖和细胞周期进程，Rock抑制剂可阻滞TNF-α对VSMCs的这一作用，Rock信号通路参与TNF-α诱导的VSMCs增殖和细胞周期进程加速。

Abstract

Objective To investigate the role of Rho kinase (Rock) signaling pathway in the proliferation of rabbit basilar artery vascular smooth muscle cells (VSMCs) stimulated by tumor necrosis factor-α (TNF-α). Methods VSMCs were isolated and primarily cultured from rabbit basilar artery. After Y-27632

Rock inhibitor at different concentrations was added into the culture

medium

followed by the stimulation of TNF-α. Cell viability was determined with CCK-8 assay. Cell cycle of VSMCs was determined by flow cytometry. The expression of proliferating cell nuclear antigen (PCNA) in the VSMCs was measured with immunocytochemical staining and Western blot analysis. Results Compared with normal control cells

TNF-α treatment at 20 ng/mL for 24 h markedly induced VSMCs proliferation (

0.01)

increased the percentage of cells in S and G

/M phase (

0.05)

and enhanced PCNA expression (

0.01). Pretreatment with Y-27632

however

significantly reversed the above effects induced by 20 ng/mL TNF-α

that is

significantly inhibited cell proliferation (

0.01)

decreased expression of PCNA protein (

0.05) and the amounts of cells at S and G

/M phase (

0.05). Conclusion TNF-α promotes the proliferation of VSMCs and cell cycle

but Rock inhibitor

Y-27632

effectively reverses the above effects induced by TNF-α. Rock signaling pathway mediates TNF-α-induced acceleration of VSMCs proliferation and cell cycle progression.

关键词

Keywords

references

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Long non-coding RNA GASL1 promotes proliferation of vascular smooth muscle cells through Wnt/β-catenin signaling pathway

Inhibitory effect and mechanism of human umbilical cordmesenchymal stem cells on T cell activation-mediated proliferation of pulmonary artery smooth muscle cells

6-shogaol suppresses vascular smooth muscle cells proliferation by inhibiting TLR4/NF-κB signaling pathway

Effect of TRPV1 activation on proliferation of vascular smooth muscle cells in spontaneously hypertensive rats

D₃ dopamine receptor suppresses aldosterone-induced proliferation of vascular smooth muscle cells

Related Author

刘彦廷

罗然

孙拯

田春雷

王雄伟

吴西军

刘庆

刘俊峰

Related Institution

Center for Dermatology and Medical Cosmetology， the Second Affiliated Hospital of Chongqing Medical University

Department of Dermatology， Plastic and Cosmetic Surgery， Wushan People’s Hospital

Faculty of Hepatobiliary and Pancreatic Surgery， First Medical Center， General Hospital of PLA， Beijing, Air Force Medical Center of PLA

Department of Hepatobiliary Surgery， Eighth Medical Center， General Hospital of PLA， Beijing, Air Force Medical Center of PLA

Department of Radiation Oncology, Air Force Medical Center of PLA

⁰