HBx and its mutant X17-3 regulates YAP through Hippo signaling pathway in human hepatic L02 cells

陈琰琰; 朱慧芬; 杨道锋; 刘晓聪; 邓科兰; 黄春梅; 胡晓萌; 姜素华; 沈关心

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HBx and its mutant X17-3 regulates YAP through Hippo signaling pathway in human hepatic L02 cells

更新时间：2026-01-09

- HBx and its mutant X17-3 regulates YAP through Hippo signaling pathway in human hepatic L02 cells
- Journal of Army Medical University Vol. 35, Issue 16, Pages: 1704-1707(2013)
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  CLC： R322.47;R329.26;R394.2
- Published：2013
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陈琰琰,朱慧芬,杨道锋,刘晓聪,邓科兰,黄春梅,胡晓萌,姜素华,沈关心.HBx及其突变体X17-3对Hippo信号途径表达的影响[J].陆军军医大学学报(原第三军医大学学报),2013,35(16):1704-1707.

　Chen Yanyan,Zhu Huifen,Yang Daofeng,Liu Xiaocong,Deng Kelan,Huang Chunmei,Hu Xiaomeng,Jiang Suhua,Shen Guanxin.HBx and its mutant X17-3 regulates YAP through Hippo signaling pathway in human hepatic L02 cells[J].J Amry Med Univ (J Third Mil Med Univ),2013,35(16):1704-1707.
陈琰琰,朱慧芬,杨道锋,刘晓聪,邓科兰,黄春梅,胡晓萌,姜素华,沈关心.HBx及其突变体X17-3对Hippo信号途径表达的影响[J].陆军军医大学学报(原第三军医大学学报),2013,35(16):1704-1707. DOI：

　Chen Yanyan,Zhu Huifen,Yang Daofeng,Liu Xiaocong,Deng Kelan,Huang Chunmei,Hu Xiaomeng,Jiang Suhua,Shen Guanxin.HBx and its mutant X17-3 regulates YAP through Hippo signaling pathway in human hepatic L02 cells[J].J Amry Med Univ (J Third Mil Med Univ),2013,35(16):1704-1707. DOI：

摘要

目的初步探寻HBx及其不同突变体表达与Hippo信号途径的关系，以及对细胞凋亡的影响。方法 HBx及其突变体载体转染人正常肝细胞系L02，转染48 h后，提取总蛋白，Western blot检测细胞中Hippo信号途径MST1、YAP(yes-associated protein)的表达，磷酸化和去磷酸化的情况。采用Annexin V/PI标记流式细胞术检测细胞凋亡。结果 HBx及其突变体载体转染L02细胞48 h后，Western blot结果显示细胞MST1表达下调，p-MST1/2表达上升（

0.05）；YAP表达上升，p-YAP表达下调（

0.05）。Annexin V/PI标记法流式细胞术结果显示HBx及其突变体能促进L02细胞发生凋亡（

0.05），其介导的细胞早期凋亡尤为明显。结论 HBx通过Hippo信号通路途径调控下游致癌基因YAP的表达。结合HBx介导的L02细胞凋亡这一结果说明HBx可能通过多种途径调节细胞周期。

Abstract

Objective To investigate the relationship of HBx and its mutant X17-3 with Hippo signaling pathway

and the effects of HBx on cell cycle and apoptosis. Methods L02 cells were transfected with HBx and its mutant

respectively. After 48 h of incubation

the expression levels as well as the phosphorylation status of MST1/2 and yes-associated protein (YAP) were detected by Western blotting. The apoptosis of L02 cells was examined with flow cytometry by Annexin V/PI staining. Results After 48 hours’ transfection

Western blotting showed that the expression levels of p-MST1/2 and

YAP were up-regulated

and those of MST1/2 and p-YAP were down-regulated (

0.05). The results of flow cytometry indicated that the transfection of HBx and X17-3 resulted in more apoptotic cells （

0.05）

especially in early stage of apoptosis. Conclusion HBx regulates its downstream oncogene

YAP through Hippo signaling pathway. Its also regulates the cell cycle via several ways.

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