Interaction of indoleamine 2, 3-dioxygenase and CD4+CD25+Foxp3+ regulatory T cell in asthmatic mice

周丽蓉; 张劼; 罗永艾

您当前的位置：

首页 >

文章列表页 >

Interaction of indoleamine 2, 3-dioxygenase and CD4⁺CD25⁺Foxp3⁺ regulatory T cell in asthmatic mice

更新时间：2026-01-09

- Interaction of indoleamine 2, 3-dioxygenase and CD4⁺CD25⁺Foxp3⁺ regulatory T cell in asthmatic mice
- Journal of Army Medical University Vol. 35, Issue 13, Pages: 1384-1387(2013)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：
  CLC： R392.32;R345;R562.25
- Published：2013
- 稿件说明：
移动端阅览
周丽蓉,张劼,罗永艾.IDO与Treg在支气管哮喘小鼠中的相互作用及其意义[J].陆军军医大学学报(原第三军医大学学报),2013,35(13):1384-1387.

　Zhou Lirong,Zhang Jie,Lou Yongai.Interaction of indoleamine 2, 3-dioxygenase and CD4+CD25+Foxp3+ regulatory T cell in asthmatic mice[J].J Amry Med Univ (J Third Mil Med Univ),2013,35(13):1384-1387.
周丽蓉,张劼,罗永艾.IDO与Treg在支气管哮喘小鼠中的相互作用及其意义[J].陆军军医大学学报(原第三军医大学学报),2013,35(13):1384-1387. DOI：

　Zhou Lirong,Zhang Jie,Lou Yongai.Interaction of indoleamine 2, 3-dioxygenase and CD4+CD25+Foxp3+ regulatory T cell in asthmatic mice[J].J Amry Med Univ (J Third Mil Med Univ),2013,35(13):1384-1387. DOI：

摘要

目的探讨吲哚胺2，3双加氧酶(indoleamine 2

3-dioxygense，IDO) 与CD4

CD25

Foxp3

调节性T细胞（Treg）之间的相关性及在支气管哮喘发病机制中的作用。方法 BALB/c小鼠用随机数字表法分成对照组和哮喘组，每组8只。哮喘组以鸡卵清蛋白（ovalbumin

OVA）致敏，激发小鼠建立哮喘模型，无创肺功能仪检测气道反应性，支气管肺泡灌洗液(BALF)进行细胞学分析，ELISA检测BALF中INF-γ、IL-4、IL-10浓度，实时荧光定量PCR检测肺组织IDO和Foxp3 mRNA表达，免疫组织化学方法检测IDO蛋白表达，流式细胞仪检测Treg占CD4

细胞的百分率。结果哮喘组小鼠气道反应性、BALF中细胞总数、嗜酸性粒细胞比例及IL-4浓度明显高于对照组(

0.01)；而INF-γ与IL-10浓度、IDO和Foxp3的mRNA表达、IDO蛋白表达、Treg占CD4

细胞的百分率明显低于对照组(

0.01)；对照组与哮喘组IDO与Foxp3的mRNA表达呈正相关（

=0.819，r=0.807

0.05），对照组与哮喘组IDO蛋白表达与Treg占CD4

细胞的百分率呈正相关（

=0.783

=0.765

0.05）。结论哮喘小鼠IDO和Foxp3表达降低，Treg数量减少，且IDO蛋白表达与Treg占CD4

细胞的百分率呈正相关，表明IDO与Treg相互调节，打破免疫耐受，诱导哮喘发生。

Abstract

Objective To explore the interaction and the role of indoleamine 2

3-dioxygenase (IDO) and CD4

CD25

Foxp3

regulatory T cell (Treg) in a mice model of allergic bronchial asthma. Methods BALB/c mice were sensitized an

d challenged by ovalbumin (OVA). Penh were measured to evaluate the airway responsiveness by noninvasive lung functional instrument. Bronchoalveolar lavage cytology was analyzed. IFN-γ

IL-4 and IL-10 in BALF were detected by enzyme-linked immunosorbent assay (ELISA). The mRNA expression of IDO and Foxp3 was measured by real-time fluorescence-based quantitative PCR. The protein expression of IDO was detected by immunohistochemistry. The percentage of Treg in CD4

cells was assessed by flow cytometry. Results The airway responsiveness

the total cell number

the eosinophils and IL-4 in BALF of the asthmatic group significantly increased as compared with the control group (

0.01). The levels of IFN-γ and IL-10 in BALF

the mRNA expression of IDO and Foxp3

the protein expression of IDO

and the percentage of Treg in CD4

cells in the asthmatic group were significantly lower than those in the control group (

0.01). The mRNA expression of IDO and Foxp3 was positively correlated with each other (

=0.819

0.807

0.05). The protein expression of IDO was positively correlated with the percentage of Treg in CD4

cells (

=0.783

0.765

P0.05). Conclusions IDO and Treg reciprocally regulate each other

which surmounts immune tolerance and induces asthma. Therefore

IDO and Treg may play important roles in asthma.

关键词

Keywords

references

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

IL-17A inhibits Th2 cell differentiation and alleviates eosinophilic airway inflam-mation in a mouse model of asthma

Establishment of mouse model of immune tolerance of asthma through subcutaneous injection of high dose of ovalbumin

Effect of maternal 1α,25-dihydroxyvitamin D3 supplement on allergic airway inflammation of its offspring in mouse asthma model

Analysis of anxiety and depression in asthma outpatients

Human regulatory T cells exert inhibitory function on CD4⁺ effector T cells through interaction between herpes virus entry mediator and CD160

Related Author

王长征

蒋云秋

郭东霖

王冉

杨旭

赵生涛

王熠杰

汪金玉

Related Institution

No data

⁰