Heme oxygenase-1 protects rats against myocardial injury after resuscitation

王晓红; 周文杰; 杨晓军; 方向韶; 黄子通

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Heme oxygenase-1 protects rats against myocardial injury after resuscitation

更新时间：2026-01-09

- Heme oxygenase-1 protects rats against myocardial injury after resuscitation
- Journal of Army Medical University Vol. 35, Issue 18, Pages: 1947-1951(2013)
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  CLC： R322.11;R392.3;R605.974
- Published：2013
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王晓红,周文杰,杨晓军,方向韶,黄子通.血红素氧合酶-1对大鼠复苏后心肌损伤的保护作用和机制[J].陆军军医大学学报(原第三军医大学学报),2013,35(18):1947-1951.

　Wang Xiaohong,Zhou Wenjie,Yang Xiaojun,Fang Xiangshao,Huang Zitong.Heme oxygenase-1 protects rats against myocardial injury after resuscitation[J].J Amry Med Univ (J Third Mil Med Univ),2013,35(18):1947-1951.
王晓红,周文杰,杨晓军,方向韶,黄子通.血红素氧合酶-1对大鼠复苏后心肌损伤的保护作用和机制[J].陆军军医大学学报(原第三军医大学学报),2013,35(18):1947-1951. DOI：

　Wang Xiaohong,Zhou Wenjie,Yang Xiaojun,Fang Xiangshao,Huang Zitong.Heme oxygenase-1 protects rats against myocardial injury after resuscitation[J].J Amry Med Univ (J Third Mil Med Univ),2013,35(18):1947-1951. DOI：

摘要

目的探讨血红素氧合酶-1（heme oxygenase-1

HO-1）抗炎效应对大鼠复苏后心肌损伤的影响。方法采用窒息法制作大鼠心跳骤停心肺复苏（cardiopulmonary resuscitation，CPR）模型。60只雄性SD大鼠分为4组：假手术组（仅置入相关导管，不行CPR术）、CPR组、Hemin组（血晶素组，Hemin 30 mg/kg 于CPR前18 h及2 h 2次腹腔注射）、Hemin+ZnPP组（血晶素+锌原卟啉组，Hemin 用法同前，ZnPP 5 mg/kg于CPR前17 h和1 h 2次腹腔注射）。各组术后6、24 h 2个观察时相点，假手术组每时点6只鼠，各复苏组每时相点8只鼠。Western blot法检测各组心肌组织HO-1蛋白水平；全自动生化分析仪测定血清肌酸肌酶同工酶（CK-MB）、乳酸脱氢酶（LDH）含量；ELISA法测定大鼠血清TNF-α、IL-6浓度；透射电镜下观察心肌超微结构变化。结果 ①各复苏组复苏后6、24 h HO-1蛋白表达水平增高，且Hemin组HO-1水平高于CPR组和Hemin+ZnPP组（

0.05）。②各复苏组复苏后6、24 h CK-MB和LDH活性增高，且Hemin组各时相点CK-MB和LDH活性均低于CPR组和Hemin+ZnPP组（

0.05）。③TNF-α水平：CPR组、Hemin+ZnPP组复苏后6、24 h均升高，Hemin组仅复苏后6 h升高，且低于前2组（

0.05）；IL-6水平：CPR组、Hemin+ZnPP组仅复苏后24 h升高（P0.05），Hemin组复苏后各时相点未见升高（

0.05）。④与假手术组比较，各复苏组大鼠心肌超微结构损伤明显，但Hemin组心肌超微结构完整性优于CPR组和Hemin+ZnPP组。结论 HO-1可减轻大鼠心肺复苏后心肌损伤，其抗炎效应可能是其保护机制之一。

Abstract

Objective To determine the anti-inflammatory effects of heme oxygenase-1 (HO-1) on post-resuscitation myocardial injury in rats. Methods The rat model of cardiopulmonary resuscitation (CPR) were established by being asphyxiated f

or 9 min and resuscitated. A total of 60 male Sprague-Dawley rats

weighing 330-380 g

were randomly divided into 4 groups

that is

sham-operation group (placement of related catheter without cardiopulmonary resuscitation)

CPR group

Hemin treatment group (Hemin

30 mg/kg

given in 2 and 18 h before CPR infliction by intraperitoneal injection)

and Hemin+zinc protoporphyrin IX (ZnPP) treatment group (Hemin given as precious description. ZnPP

5 mg/kg

given in 1 and 17 h before CPR infliction by intraperitoneal injection). Resuscitated groups were observed in 6 h and 24 h after cardio-pulmonary resuscitation (with 8 rats for each time point). Sham-operation group (n=12) were observed at the same time points with 6 rats for each time point. HO-1 expression in cardiac tissue was detected by Western blotting. Serum creatine kinase isoenzyme (CK-MB) and lactate dehydrogenase (LDH) levels were detected by automatic biochemistry analyzer. Serum concentrations of interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) were determined by enzyme-linked immunosorbent assay (ELISA). Cardiac ultrastructure was observed by transmission electron microscopy. Results The expression of HO-1 in resuscitated groups were markedly increased in 6 and 24 h after resuscitation. The level of HO-1 was significantly higher in Hemin group than that in CPR group and Hemin+ZnPP group at the 2 time points after resuscitation (all

0.05). Serum levels of CPK-MB and LDH in resuscitated groups were markedly increased at 6 and 24 h after resuscitation. The activity of CPK-MB and LDH in Hemin group were significantly lower than that in CPR group and Hemin+ZnPP group (all

0.05）. The level of TNF-α were significantly increased in CPR group and Hemin+ZnPP group at 6 and 24 h after resuscitation（all

0.05）; but that in Hemin group was increased only in 6 h after resuscitation and lower than the former 2 groups（all

0.05）

and then returned to baseline in 24 h after resuscitation（

0.05）. The level of IL

-6 were significantly increased in CPR group and Hemin+ZnPP group only in 24 h after resuscitation（

0.05）

but that in Hemin group had no significant difference after resuscitation（

0.05）. The cardiac tissue ultrastructure of rats in Hemin group was more intact than CPR group and Hemin+ZnPP group. Conclusion HO-1 attenuates myocardial injury in rats after cardiopulmonary resuscitation

probably through its anti-inflammatory effect.

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