Objective       To establish a Th17-mediated allergic asthma mouse model with increased neutrophils around airways.       Methods      Ovalbumin (OVA) and lipopolysaccharide (LPS) were applied for sensitization to establish the new allergic asthma mouse model. Airway hyperresponsiveness (AHR) was evaluated at 24 h after the last airway challenge. The subtypes and numbers of inflammatory cells in bronchoalveolar lavage fluid (BALF) were counted. The pathological changes of lung tissues were observed by HE staining. Q-PCR was applied to detect the shift of Th1

Th2 and Th17 cells in lung tissues.       Results      OVA and LPS combined sensitization induced strong AHR response. Neutrophils (28.63±8.89)% and eosinophils (16.09±4.42)% were the main subtypes of inflammatory cells in BALF

and the lung tissues presented obvious asthmatic pathological changes. Q-PCR results indicated that naive T cells mainly shifted to Th2 cells when the mice were sensitized by OVA alone

while those mainly shifted to Th17 cells when the mice were sensitized by OVA combined with LPS.       Conclusion      The Th17-mediated allergic asthma mouse model with neutrophils as major inflammatory cells around airways was successfully established.