Effect of HUVEC injury induced by cholesterol on VSMC proliferation and migration

杨一峻; 罗洁; 钱民章

您当前的位置：

首页 >

文章列表页 >

Effect of HUVEC injury induced by cholesterol on VSMC proliferation and migration

更新时间：2026-01-09

- Effect of HUVEC injury induced by cholesterol on VSMC proliferation and migration
- Journal of Army Medical University Vol. 35, Issue 13, Pages: 1362-1365(2013)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：
  CLC： R322.12;R329.28;R344.85
- Published：2013
- 稿件说明：
移动端阅览
杨一峻,罗洁,钱民章.胆固醇致内皮细胞损伤对平滑肌细胞增殖和迁移的促进作用[J].陆军军医大学学报(原第三军医大学学报),2013,35(13):1362-1365.

　Yang Yijun,Luo Jie,Qian Minzhang.Effect of HUVEC injury induced by cholesterol on VSMC proliferation and migration[J].J Amry Med Univ (J Third Mil Med Univ),2013,35(13):1362-1365.
杨一峻,罗洁,钱民章.胆固醇致内皮细胞损伤对平滑肌细胞增殖和迁移的促进作用[J].陆军军医大学学报(原第三军医大学学报),2013,35(13):1362-1365. DOI：

　Yang Yijun,Luo Jie,Qian Minzhang.Effect of HUVEC injury induced by cholesterol on VSMC proliferation and migration[J].J Amry Med Univ (J Third Mil Med Univ),2013,35(13):1362-1365. DOI：

摘要

目的观察胆固醇损伤人血管内皮细胞（human umbilical vein endothelial cell ，HUVEC）后能否影响血管平滑肌（vascular smooth muscle，VSMC）的增殖与迁移。方法用12.5、25.0、50.0 mg/L 3个浓度的胆固醇作用体外培养的HUVEC 24 h后，再用培养内皮细胞的培养基继续培养VSMC 24 h，CCK-8、划痕实验检测VSMC增殖与迁移；用12.5、25.0、50.0 mg/L 的胆固醇作用共培养的HUVEC、VSMC 24 h，用Transwell检测VSMC迁移。结果 25.0、50.0 mg/L胆固醇作用HUVEC后的培养基与对照组相比（1.37±0.01），明显促进VSMC增殖（1.53±0.06）、（1.54±0.10）(

0.05)；12.5、25.0、50.0 mg/L胆固醇作用HUVEC后的培养基与对照组相比（33.24±0.43）均能促进VSMC细胞迁移[(269.10±3.78)、(272.79±4.69)、(458.69 ± 4.78) ，

0.01

]

。胆固醇作用共培养的HUVEC、VSMC 24 h后，促进VSMC迁移[（175.00±4.63）、（182.00±4.13）、(207.00±5.59)

]

，与对照组相比(101.00±2.33)差异明显(

0.01)。结论胆固醇诱导HUVEC损伤后能够促进VSMC增殖与迁移。

Abstract

Objective To observe the effect of human umbilical vein endothelial cell (HUVEC) injury induced by cholesterol on human aortic vascular endothelial cell (HA-VSMC) proliferation and migration. Methods HUVEC were treated with cholesterol at 12.5

25.0 and 50.0 mg/L for 24 h

and then the VSMCs were incubated with the medium of cultured HUVEC for 24 h. CCK-8 and scratch assay was applied to analyze VSMC viability

and migration. HUVEC and VSMC was co-cultured and treated with cholesterol at 12.5

25.0 and 50.0mg/L for 24 h. VSMC migration was detected by Transwell assay. Results Compared with the control group (1.37±0.01)

the proliferation of VSMCs incubated with the culture medium of HUVEC treated with cholesterol at 25.0 mg/L and 50.0 mg/L increased significantly (1.53±0.06 and 1.54±0.10

0.05). The culture medium of HUVEC subjected to cholesterol at 12.5

25.0 and 50.0 mg/L could induce VSMC migration (269.10±3.78

272.79±4.69 and 458.69±4.78)

which were significantly different from that of the control group (33.24±0.43

0.01). The co-culture of HUVEC and VSMC treated by cholesterol induced VSMC migration (175.00±4.63

182.00±4.13 and 207.00±5.59)

which were significantly different from that of the control group (101.00±2.33

0.01). Conclusion HUVEC

which is injured by cholesterol

can induce VSMC proliferation and migration.

关键词

Keywords

references

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Effect of rosiglitazone on expression of acyl-coenzyme A cholesterol acyltransferase 1 and ATP-binding cassette transporter A1 in foam cells

Construction and verification of a nomogram prediction model for vascular plaque stability in patients with progressive cerebral infarction based on serum MCP-1, MCPIP1 combined with inflammatory factors

Dihydromyricetin ameliorates vascular endothelial damage by inhibiting NLRP3 inflammasome activity through Sirtuin 3

Melatonin inhibits ox-LDL-induced proliferation of vascular smooth muscle cells

Construction of an inflammatory microenvironment-responsive HDL-mimetic nanoparticle and evaluation of its in vitro anti-atherosclerotic activity

Related Author

蒙颖

徐芳

王志禄

李万玲

贾军正

郭文芬

谢宛霞

胡海英

Related Institution

Department of Neurology， Nanyang Second People’s Hospital， Xinxiang Medical College

⁰