Objective       To determine the efficiency of over-expression of MAD2B gene on biological functions of dermal papilla cells (DPCs).       Methods       Eukaryotic expression plasmid vectors pIRES2-MAD2B and pIRES2-T cell factor 4 (TCF4) were constructed and transfected in the DPCs with Lipofectamine 2000. The transfection efficiency was eval

uated by green fluorescence microscopy

and the objective proteins were detected with Western blotting. Further

the interaction of 2 proteins was verified with co-immunoprecipitation analysis

and the change of cell proliferation and signal strength of Wnt signal pathway

DPFs secreting insulin-like growth factor-1 (IGF-1)

vascular endothelial growth factor (VEGF) and hepatocyte growth factor (HGF) were detected with real-time PCR.       Results       MAD2B and TCF4 genes were expressed in DPFs successfully. Co-immunoprecipitation analysis suggested that there was interaction between the 2 proteins in DPFs. Wnt signaling pathway was reduced in MAD2B-transfected DPFs compared with the control and TCF4-transfected DPFs (

P

0.05). The proliferation was declined in the MAD2B-transfected DPFs than in TCF4-transfected DPFs (

P

0.05). The mRNA expression of IGF-1

VEGF

and HGF was also decreased in the MAD2B-transfected DPFs.       Conclusion       MAD2B has an interaction with TCF4 in DPFs

and its overexpression might inhibit cell proliferation and reduce the expression of IGF-1

VEGF and HGF by Wnt signaling pathway.